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Effects of ex vivo ischemia time and delayed processing on quality of specimens in tissue biobank

  • Authors:
    • Dan Guo
    • Anqi Wang
    • Ting Xie
    • Sumei Zhang
    • Dingyan Cao
    • Jian Sun
  • View Affiliations / Copyright

    Affiliations: Clinical Biobank, Department of Medical Research Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4278-4288
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    Published online on: September 10, 2020
       https://doi.org/10.3892/mmr.2020.11503
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Abstract

The RNA quality of tissue biobank is crucial for translational research; however, the effects of the ex vivo ischemia time on RNA integrity and expression of genes related to hypoxia, stress, apoptosis and autophagy remains elusive. A total of 18 carcinoma tissues were stored at room temperature for 15 min, 30 min, 1, 2, 4, 8 and 24 h. The integrity and purity of isolated RNA were analyzed. Furthermore, the gene expression of mTOR, hypoxia‑inducible factor 1α, phosphatidylinositol 4,5‑bisphosphate 3‑kinase catalytic subunit β isoform (PI3KCB), threonine kinase 1 (AKT1), NF‑κB, protein kinase AMP‑activated catalytic subunit α1 (AMPKα1), caspase 8 (CASP8), unc‑51 like autophagy activating kinase 1 and Fas cell surface death receptor were analyzed using reverse transcription‑quantitative PCR. The results demonstrated that RNA integrity numbers (RINs) remained stable in carcinoma tissues following ex vivo ischemia for 2 h at room temperature and that degradation began at 4 h (P<0.001). Additionally, the expression of PI3KCB, AKT1, AMPKα1 and CASP8 decreased at time points 8‑24 h following ex vivo ischemia and delayed processing (P<0.001). In conclusion, >2 h of ex vivo ischemia and delayed processing induced RNA degradation and RIN, and the gene expressions of PI3KCB, AKT, AMPKα1 and CASP8 may be considered as markers to evaluate tissue quality at the gene expression level, providing a method for the standard processing and assessment of tissue specimen.
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Copy and paste a formatted citation
Spandidos Publications style
Guo D, Wang A, Xie T, Zhang S, Cao D and Sun J: Effects of ex vivo ischemia time and delayed processing on quality of specimens in tissue biobank. Mol Med Rep 22: 4278-4288, 2020.
APA
Guo, D., Wang, A., Xie, T., Zhang, S., Cao, D., & Sun, J. (2020). Effects of ex vivo ischemia time and delayed processing on quality of specimens in tissue biobank. Molecular Medicine Reports, 22, 4278-4288. https://doi.org/10.3892/mmr.2020.11503
MLA
Guo, D., Wang, A., Xie, T., Zhang, S., Cao, D., Sun, J."Effects of ex vivo ischemia time and delayed processing on quality of specimens in tissue biobank". Molecular Medicine Reports 22.5 (2020): 4278-4288.
Chicago
Guo, D., Wang, A., Xie, T., Zhang, S., Cao, D., Sun, J."Effects of ex vivo ischemia time and delayed processing on quality of specimens in tissue biobank". Molecular Medicine Reports 22, no. 5 (2020): 4278-4288. https://doi.org/10.3892/mmr.2020.11503
Copy and paste a formatted citation
x
Spandidos Publications style
Guo D, Wang A, Xie T, Zhang S, Cao D and Sun J: Effects of ex vivo ischemia time and delayed processing on quality of specimens in tissue biobank. Mol Med Rep 22: 4278-4288, 2020.
APA
Guo, D., Wang, A., Xie, T., Zhang, S., Cao, D., & Sun, J. (2020). Effects of ex vivo ischemia time and delayed processing on quality of specimens in tissue biobank. Molecular Medicine Reports, 22, 4278-4288. https://doi.org/10.3892/mmr.2020.11503
MLA
Guo, D., Wang, A., Xie, T., Zhang, S., Cao, D., Sun, J."Effects of ex vivo ischemia time and delayed processing on quality of specimens in tissue biobank". Molecular Medicine Reports 22.5 (2020): 4278-4288.
Chicago
Guo, D., Wang, A., Xie, T., Zhang, S., Cao, D., Sun, J."Effects of ex vivo ischemia time and delayed processing on quality of specimens in tissue biobank". Molecular Medicine Reports 22, no. 5 (2020): 4278-4288. https://doi.org/10.3892/mmr.2020.11503
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