Resveratrol decreases high glucose‑induced apoptosis in renal tubular cells via suppressing endoplasmic reticulum stress

Zhang,Jing; Dong,Xiong‑Jun; Ding,Meng‑Ru; You,Chun‑Yu; Lin,Xin; Wang,Ying; Wu,Miao‑Jie‑Yang; Xu,Guo‑Fei; Wang,Guo‑Dong

doi:10.3892/mmr.2020.11511

Resveratrol decreases high glucose‑induced apoptosis in renal tubular cells via suppressing endoplasmic reticulum stress

Authors:
- Jing Zhang
- Xiong‑Jun Dong
- Meng‑Ru Ding
- Chun‑Yu You
- Xin Lin
- Ying Wang
- Miao‑Jie‑Yang Wu
- Guo‑Fei Xu
- Guo‑Dong Wang
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Affiliations: Department of Nephrology, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China, Department of Nephrology, The Second People's Hospital of Wuhu, Wuhu, Anhui 241000, P.R. China, Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro‑Molecules, Wuhu, Anhui 241002, P.R. China, School of Medical Imaging, Wannan Medical College, Wuhu, Anhui 241002, P.R. China
Published online on: September 15, 2020 https://doi.org/10.3892/mmr.2020.11511
Pages: 4367-4375
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Diabetic nephropathy (DN) is the second most common complication of diabetes mellitus after cardiovascular complications. Endoplasmic reticulum (ER) stress is known to be associated with DN. Resveratrol (RSV) exhibits anti‑oxidative, anti‑inflammatory and cytoprotective effects. Therefore, the aims of the present study were to investigate the role of RSV in the inhibition of high concentration glucose (HG)‑induced apoptosis in renal tubular cells, as well as to examine the protective effects of RSV against diabetes‑mediated renal damage via inhibition of ER stress in DN. RSV was orally administered to diabetic db/db mice once a day for 12 consecutive weeks. Compared with untreated db/db mice, treating db/db mice with RSV significantly decreased urine albumin excretion and the urine albumin to creatinine ratio, and attenuated renal histopathological injury. Furthermore, RSV treatment resulted in decreased expression levels of glucose‑regulated protein of 78 kDa and C/EBP‑homologous protein (two ER stress markers) and caspase12 in murine kidneys. RSV administration also inhibited the apoptosis of NRK‑52E cells and activation of the ER stress signal transduction pathway induced by HG treatment in vitro. Collectively, the present results indicated that RSV protected renal tubular cells against HG‑induced apoptosis in DN by suppressing ER stress.

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