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Article Open Access

miR‑124‑3p regulates angiogenesis in peripheral arterial disease by targeting STAT3

  • Authors:
    • Yefei Shi
    • Xu Xu
    • Peipei Luan
    • Wenxin Kou
    • Mingjie Li
    • Qing Yu
    • Jianhui Zhuang
    • Yawei Xu
    • Wenhui Peng
    • Weixia Jian
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China, Department of Endocrinology, Xinhua Hospital Affiliated to Shanghai Jiaotong University, School of Medicine, Shanghai 200092, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4890-4898
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    Published online on: September 25, 2020
       https://doi.org/10.3892/mmr.2020.11538
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Abstract

Peripheral arterial disease (PAD) is the third leading cause of cardiovascular morbidity worldwide, after coronary artery disease and stroke. As endogenous regulators of gene expression, microRNAs (miRs) are implicated in the development and progression of various diseases, including types of cancer, autoimmune diseases and heart diseases. In the present study, the role of miR‑124‑3p in PAD was investigated. The reverse transcription‑quantitative PCR results indicated that the expression levels of miR‑124‑3p were significantly increased in the ischemic tissue of the hindlimb ischemia (HLI) model and in hypoxic human umbilical vein endothelial cells compared with the corresponding control groups. Proliferation, wound healing and tube formation assays demonstrated the inhibition of miR‑124‑3p on angiogenesis in vitro and the HLI model indicated the same function of miR‑124‑3p in vivo. A dual‑luciferase reporter revealed STAT3 as the target of miR‑124‑3p. The expression levels of miR‑124‑3p in human blood were negatively correlated with ankle‑brachial index, which is an index for the evaluation of the severity of PAD. Collectively, the present study indicated that miR‑124‑3p was a critical regulator of angiogenesis in PAD, and a potential diagnostic, prognostic and therapeutic target for PAD.
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Copy and paste a formatted citation
Spandidos Publications style
Shi Y, Xu X, Luan P, Kou W, Li M, Yu Q, Zhuang J, Xu Y, Peng W, Jian W, Jian W, et al: miR‑124‑3p regulates angiogenesis in peripheral arterial disease by targeting STAT3. Mol Med Rep 22: 4890-4898, 2020.
APA
Shi, Y., Xu, X., Luan, P., Kou, W., Li, M., Yu, Q. ... Jian, W. (2020). miR‑124‑3p regulates angiogenesis in peripheral arterial disease by targeting STAT3. Molecular Medicine Reports, 22, 4890-4898. https://doi.org/10.3892/mmr.2020.11538
MLA
Shi, Y., Xu, X., Luan, P., Kou, W., Li, M., Yu, Q., Zhuang, J., Xu, Y., Peng, W., Jian, W."miR‑124‑3p regulates angiogenesis in peripheral arterial disease by targeting STAT3". Molecular Medicine Reports 22.6 (2020): 4890-4898.
Chicago
Shi, Y., Xu, X., Luan, P., Kou, W., Li, M., Yu, Q., Zhuang, J., Xu, Y., Peng, W., Jian, W."miR‑124‑3p regulates angiogenesis in peripheral arterial disease by targeting STAT3". Molecular Medicine Reports 22, no. 6 (2020): 4890-4898. https://doi.org/10.3892/mmr.2020.11538
Copy and paste a formatted citation
x
Spandidos Publications style
Shi Y, Xu X, Luan P, Kou W, Li M, Yu Q, Zhuang J, Xu Y, Peng W, Jian W, Jian W, et al: miR‑124‑3p regulates angiogenesis in peripheral arterial disease by targeting STAT3. Mol Med Rep 22: 4890-4898, 2020.
APA
Shi, Y., Xu, X., Luan, P., Kou, W., Li, M., Yu, Q. ... Jian, W. (2020). miR‑124‑3p regulates angiogenesis in peripheral arterial disease by targeting STAT3. Molecular Medicine Reports, 22, 4890-4898. https://doi.org/10.3892/mmr.2020.11538
MLA
Shi, Y., Xu, X., Luan, P., Kou, W., Li, M., Yu, Q., Zhuang, J., Xu, Y., Peng, W., Jian, W."miR‑124‑3p regulates angiogenesis in peripheral arterial disease by targeting STAT3". Molecular Medicine Reports 22.6 (2020): 4890-4898.
Chicago
Shi, Y., Xu, X., Luan, P., Kou, W., Li, M., Yu, Q., Zhuang, J., Xu, Y., Peng, W., Jian, W."miR‑124‑3p regulates angiogenesis in peripheral arterial disease by targeting STAT3". Molecular Medicine Reports 22, no. 6 (2020): 4890-4898. https://doi.org/10.3892/mmr.2020.11538
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