Open Access

Integrated microarray analysis of key genes and a miRNA‑mRNA regulatory network of early‑onset preeclampsia

  • Authors:
    • Hao Zhang
    • Lu Xue
    • Yan Lv
    • Xiang Yu
    • Yiwei Zheng
    • Zhijing Miao
    • Hongjuan Ding
  • View Affiliations

  • Published online on: September 30, 2020     https://doi.org/10.3892/mmr.2020.11551
  • Pages: 4772-4782
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Early‑onset preeclampsia (EOPE) is a serious threat to maternal and foetal health. The present study aimed to identify potential biomarkers and targets for the treatment of EOPE. Expression profiles of placenta from patients with EOPE and healthy controls (GSE103542, GSE74341 and GSE44711) were downloaded from the Gene Expression Omnibus database. Integrated analysis revealed 246 genes and 28 microRNAs (miRNAs) that were differentially expressed between patients with EOPE and healthy controls. Differentially expressed genes (DEGs) were primarily enriched in ‘biological processes’, such as ‘cell adhesion’, ‘female pregnancy’, ‘extracellular matrix organization’ and ‘response to hypoxia’. Significant pathways associated with DEGs primarily included ‘focal adhesion’, ‘ECM‑receptor interaction’, ‘PI3K‑Akt signaling’ and ‘ovarian steroidogenesis’. A Protein‑Protein Interaction network of DEGs was constructed using the Search Tool for the Retrieval of Interacting Genes/Proteins online database, and epidermal growth factor receptor, collagen α‑1(I) chain, secreted phosphoprotein 1, leptin (LEP), collagen α‑2(I) chain (COL1A2), plasminogen activator inhibitor 1 (SERPINE1), Thy‑1 membrane glycoprotein, bone morphogenetic protein 4, vascular cell adhesion protein 1 and matrix metallopeptidase 1 were identified as hub genes. The alterations of hsa‑miR‑937, hsa‑miR‑148b*, hsa‑miR‑3907, hsa‑miR‑367*, COL1A2, LEP and SERPINE1 in placenta were validated using our local samples. Our research showed that the expression of hsa‑miR‑937, hsa‑miR‑1486*, hsa‑miR‑3907, hsa‑miR‑367* and hub genes in the placenta were closely associated with the pathophysiology of EOPE. hsa‑miR‑937, hsa‑miR‑1486*, hsa‑miR‑3907, hsa‑miR‑367* and hub genes could serve as biomarkers for diagnosis and as potential targets for the treatment of EOPE.
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December-2020
Volume 22 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhang H, Xue L, Lv Y, Yu X, Zheng Y, Miao Z and Ding H: Integrated microarray analysis of key genes and a miRNA‑mRNA regulatory network of early‑onset preeclampsia. Mol Med Rep 22: 4772-4782, 2020.
APA
Zhang, H., Xue, L., Lv, Y., Yu, X., Zheng, Y., Miao, Z., & Ding, H. (2020). Integrated microarray analysis of key genes and a miRNA‑mRNA regulatory network of early‑onset preeclampsia. Molecular Medicine Reports, 22, 4772-4782. https://doi.org/10.3892/mmr.2020.11551
MLA
Zhang, H., Xue, L., Lv, Y., Yu, X., Zheng, Y., Miao, Z., Ding, H."Integrated microarray analysis of key genes and a miRNA‑mRNA regulatory network of early‑onset preeclampsia". Molecular Medicine Reports 22.6 (2020): 4772-4782.
Chicago
Zhang, H., Xue, L., Lv, Y., Yu, X., Zheng, Y., Miao, Z., Ding, H."Integrated microarray analysis of key genes and a miRNA‑mRNA regulatory network of early‑onset preeclampsia". Molecular Medicine Reports 22, no. 6 (2020): 4772-4782. https://doi.org/10.3892/mmr.2020.11551