Open Access

Cathepsin B aggravated doxorubicin‑induced myocardial injury via NF‑κB signalling

  • Authors:
    • Chen Liu
    • Zhulan Cai
    • Tongtong Hu
    • Qi Yao
    • Lijun Zhang
  • View Affiliations

  • Published online on: October 11, 2020     https://doi.org/10.3892/mmr.2020.11583
  • Pages: 4848-4856
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Myocyte apoptosis and oxidative stress key critical roles in the process of doxorubicin (DOX)‑induced cardiotoxicity. However, how apoptosis and oxidative stress arise in DOX‑induced heart injury remains largely unknown. Cathepsin B (CTSB) is a typical lysosomal cysteine protease that is associated with apoptosis, inflammatory responses, oxidative stress and autophagy. The present study aimed to investigate the role of CTSB in DOX‑induced heart injury and its potential mechanism. H9C2 cells were infected with adenovirus or transfected with small interfering RNA to overexpress or knock down CTSB, respectively, and then stimulated with DOX. DOX induced increased CTSB expression levels in H9C2 cells. DOX‑induced cardiomyocyte apoptosis and oxidative stress were attenuated by CTSB knockdown but aggravated by CTSB overexpression in vitro. Mechanistically, the present study showed that CTSB activated the NF‑κB pathway in response to DOX. In summary, CTSB aggravated DOX‑induced H9C2 cell apoptosis and oxidative stress via NF‑κB signalling. CTSB constitutes a potential therapeutic target for the treatment of DOX‑induced cardiotoxicity.
View Figures
View References

Related Articles

Journal Cover

December-2020
Volume 22 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Liu C, Cai Z, Hu T, Yao Q and Zhang L: Cathepsin B aggravated doxorubicin‑induced myocardial injury via NF‑κB signalling . Mol Med Rep 22: 4848-4856, 2020
APA
Liu, C., Cai, Z., Hu, T., Yao, Q., & Zhang, L. (2020). Cathepsin B aggravated doxorubicin‑induced myocardial injury via NF‑κB signalling . Molecular Medicine Reports, 22, 4848-4856. https://doi.org/10.3892/mmr.2020.11583
MLA
Liu, C., Cai, Z., Hu, T., Yao, Q., Zhang, L."Cathepsin B aggravated doxorubicin‑induced myocardial injury via NF‑κB signalling ". Molecular Medicine Reports 22.6 (2020): 4848-4856.
Chicago
Liu, C., Cai, Z., Hu, T., Yao, Q., Zhang, L."Cathepsin B aggravated doxorubicin‑induced myocardial injury via NF‑κB signalling ". Molecular Medicine Reports 22, no. 6 (2020): 4848-4856. https://doi.org/10.3892/mmr.2020.11583