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Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis

  • Authors:
    • Xi Chen
    • Shenying Ji
    • Jia Si
    • Xiangyu Zhang
    • Xiaoyan Wang
    • Yong Guo
    • Xianqiong Zou
  • View Affiliations / Copyright

    Affiliations: Laboratory of Mucosal Immunology, Affiliated Stomatology Hospital of Guilin Medical University, Guilin, Guangxi 541004, P.R. China, College of Biotechnology, Guilin Medical University, Guilin, Guangxi 541004, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5243-5250
    |
    Published online on: October 23, 2020
       https://doi.org/10.3892/mmr.2020.11629
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Abstract

Human cathelicidin antimicrobial peptide and its active product, LL‑37 (CAMP/LL‑37), exhibit a broad spectrum of antimicrobial effects. An increasing number of studies have shown that human CAMP/LL‑37 also serves significant roles in various types of cancer. The primary aims of the present study were to investigate the roles and mechanisms of human CAMP/LL‑37 in oral squamous cell carcinoma (OSCC) cells. The results indicated that either LL‑37 C‑terminal deletion mutants (CDEL) or CAMP stable expression in HSC‑3 cells reduced colony formation, proliferation, migration and invasion ability of the cells. Expression analysis demonstrated that either CDEL or CAMP stable expression in HSC‑3 cells induced caspase‑3 mediated apoptosis via the P53‑Bcl‑2/BAX signalling pathway, whereas the levels of cell cycle‑related proteins, cyclin B1 and PKR‑like ER kinase, were significantly upregulated in the CAMP, but not in the CDEL overexpressing cells. Transcriptional profile comparisons revealed that CDEL or CAMP stable expression in HSC‑3 cells upregulated expression of genes involved in the IL‑17‑dependent pathway compared with the control. Taken together, these results suggest that CAMP may act as a tumour suppressor in OSCC cells, and the underlying mechanism involves the induction of caspase‑3 mediated apoptosis via the P53‑Bcl‑2/BAX signalling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Ji S, Si J, Zhang X, Wang X, Guo Y and Zou X: Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis. Mol Med Rep 22: 5243-5250, 2020.
APA
Chen, X., Ji, S., Si, J., Zhang, X., Wang, X., Guo, Y., & Zou, X. (2020). Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis. Molecular Medicine Reports, 22, 5243-5250. https://doi.org/10.3892/mmr.2020.11629
MLA
Chen, X., Ji, S., Si, J., Zhang, X., Wang, X., Guo, Y., Zou, X."Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis". Molecular Medicine Reports 22.6 (2020): 5243-5250.
Chicago
Chen, X., Ji, S., Si, J., Zhang, X., Wang, X., Guo, Y., Zou, X."Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis". Molecular Medicine Reports 22, no. 6 (2020): 5243-5250. https://doi.org/10.3892/mmr.2020.11629
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Ji S, Si J, Zhang X, Wang X, Guo Y and Zou X: Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis. Mol Med Rep 22: 5243-5250, 2020.
APA
Chen, X., Ji, S., Si, J., Zhang, X., Wang, X., Guo, Y., & Zou, X. (2020). Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis. Molecular Medicine Reports, 22, 5243-5250. https://doi.org/10.3892/mmr.2020.11629
MLA
Chen, X., Ji, S., Si, J., Zhang, X., Wang, X., Guo, Y., Zou, X."Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis". Molecular Medicine Reports 22.6 (2020): 5243-5250.
Chicago
Chen, X., Ji, S., Si, J., Zhang, X., Wang, X., Guo, Y., Zou, X."Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis". Molecular Medicine Reports 22, no. 6 (2020): 5243-5250. https://doi.org/10.3892/mmr.2020.11629
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