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Downregulation of lncRNA ZFAS1 inhibits the hallmarks of thyroid carcinoma via the regulation of miR‑302‑3p on cyclin D1

  • Authors:
    • Wenjing Chen
    • Lili Zhai
    • Huiming Liu
    • Yuting Li
    • Qi Zhang
    • Dandan Xu
    • Weiye Fan
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The First Hospital of Qiqihar, Affiliated Qiqihar Hospital, Southern Medical University, Qiqihar, Heilongjiang 161000, P.R. China, Department of Thyroid Surgery, The First Hospital of Qiqihar, Affiliated Qiqihar Hospital, Southern Medical University, Qiqihar, Heilongjiang 161000, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 2
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    Published online on: November 2, 2020
       https://doi.org/10.3892/mmr.2020.11640
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Abstract

At present, treatment options for thyroid carcinoma remain limited. The present study aimed to investigate the role of ZFAS1 in various major hallmarks of cancer and the underlying mechanisms in thyroid carcinoma cells. The interactions between long non‑coding RNAs (lncRNAs), microRNAs (miRs) and target genes were predicted by bioinformatics and confirmed by performing dual‑luciferase assays. The mRNA and protein expressions were determined by reverse transcription‑quantitative PCR and western blotting. Cell invasion, migration, and viability were evaluated via Transwell, wound‑healing and Cell Counting Kit‑8 assays, respectively. The results demonstrated that lncRNA ZFAS1 expression was upregulated in thyroid carcinoma tissues, TT and SW579 cells, and was associated with the proliferation of these two cell lines. Notably, downregulation ZFAS1 reduced migration and invasion, and reversed the promotive effects of miR‑302a‑3p inhibitor on the proliferation, migration and invasion of TT and SW579 cells. Moreover, cyclin D1 (CCND1) is targeted by miR‑302a‑3p, and was regulated by ZFAS1. In addition, the downregulation of ZFAS1 not only reversed the promotive effects of miR‑302a‑3p inhibitor on CCND1 expression and the epithelial‑mesenchymal transition (EMT) of TT and SW579 cells, but also targeted and increased the expression of miR‑302a‑3p, and further reduced the expression of CCND1, resulting in suppression of the proliferation, migration, invasion and EMT of thyroid carcinoma cells.
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Copy and paste a formatted citation
Spandidos Publications style
Chen W, Zhai L, Liu H, Li Y, Zhang Q, Xu D and Fan W: Downregulation of lncRNA ZFAS1 inhibits the hallmarks of thyroid carcinoma via the regulation of miR‑302‑3p on cyclin D1. Mol Med Rep 23: 2, 2021.
APA
Chen, W., Zhai, L., Liu, H., Li, Y., Zhang, Q., Xu, D., & Fan, W. (2021). Downregulation of lncRNA ZFAS1 inhibits the hallmarks of thyroid carcinoma via the regulation of miR‑302‑3p on cyclin D1. Molecular Medicine Reports, 23, 2. https://doi.org/10.3892/mmr.2020.11640
MLA
Chen, W., Zhai, L., Liu, H., Li, Y., Zhang, Q., Xu, D., Fan, W."Downregulation of lncRNA ZFAS1 inhibits the hallmarks of thyroid carcinoma via the regulation of miR‑302‑3p on cyclin D1". Molecular Medicine Reports 23.1 (2021): 2.
Chicago
Chen, W., Zhai, L., Liu, H., Li, Y., Zhang, Q., Xu, D., Fan, W."Downregulation of lncRNA ZFAS1 inhibits the hallmarks of thyroid carcinoma via the regulation of miR‑302‑3p on cyclin D1". Molecular Medicine Reports 23, no. 1 (2021): 2. https://doi.org/10.3892/mmr.2020.11640
Copy and paste a formatted citation
x
Spandidos Publications style
Chen W, Zhai L, Liu H, Li Y, Zhang Q, Xu D and Fan W: Downregulation of lncRNA ZFAS1 inhibits the hallmarks of thyroid carcinoma via the regulation of miR‑302‑3p on cyclin D1. Mol Med Rep 23: 2, 2021.
APA
Chen, W., Zhai, L., Liu, H., Li, Y., Zhang, Q., Xu, D., & Fan, W. (2021). Downregulation of lncRNA ZFAS1 inhibits the hallmarks of thyroid carcinoma via the regulation of miR‑302‑3p on cyclin D1. Molecular Medicine Reports, 23, 2. https://doi.org/10.3892/mmr.2020.11640
MLA
Chen, W., Zhai, L., Liu, H., Li, Y., Zhang, Q., Xu, D., Fan, W."Downregulation of lncRNA ZFAS1 inhibits the hallmarks of thyroid carcinoma via the regulation of miR‑302‑3p on cyclin D1". Molecular Medicine Reports 23.1 (2021): 2.
Chicago
Chen, W., Zhai, L., Liu, H., Li, Y., Zhang, Q., Xu, D., Fan, W."Downregulation of lncRNA ZFAS1 inhibits the hallmarks of thyroid carcinoma via the regulation of miR‑302‑3p on cyclin D1". Molecular Medicine Reports 23, no. 1 (2021): 2. https://doi.org/10.3892/mmr.2020.11640
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