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Berberine alleviates palmitic acid‑induced podocyte apoptosis by reducing reactive oxygen species‑mediated endoplasmic reticulum stress

  • Authors:
    • Xing-Yang Xiang
    • Ting Liu
    • Yue Wu
    • Xu-Shun Jiang
    • Jun-Ling He
    • Xue-Mei Chen
    • Xiao-Gang Du
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, P.R. China, Department of Nephrology, Chengdu Fifth People's Hospital, Chengdu, Sichuan 611130, P.R. China, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, P.R. China, Department of Pathology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands, Emergency Department, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, P.R. China
    Copyright: © Xiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 3
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    Published online on: November 2, 2020
       https://doi.org/10.3892/mmr.2020.11641
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Abstract

Lipid accumulation in podocytes can lead to the destruction of cellular morphology, in addition to cell dysfunction and apoptosis, which is a key factor in the progression of chronic kidney disease (CKD). Berberine (BBR) is an isoquinoline alkaloid extracted from medicinal plants such as Coptis chinensis, which has been reported to have a lipid‑lowering effect and prevent CKD progression. Therefore, the present study aimed to investigate the effect of BBR on palmitic acid (PA)‑induced podocyte apoptosis and its specific mechanism using an in vitro model. Cell death was measured using the Cell Counting Kit‑8 colorimetric assay. Cell apoptotic rate was assessed by flow cytometry. The expression of endoplasmic reticulum (ER) stress‑ and apoptosis‑related proteins was detected by western blotting or immunofluorescence. Reactive oxygen species (ROS) were evaluated by 2',7'‑dichlorofluorescein diacetate fluorescence staining. The results of the present study revealed that BBR treatment decreased PA‑induced podocyte apoptosis. In addition, 4‑phenylbutyric acid significantly reduced PA‑induced cell apoptosis and the expression of ER stress‑related proteins, which indicated that ER stress was involved in PA‑induced podocyte apoptosis. In addition, N‑acetylcysteine inhibited PA‑induced excessive ROS production, ER stress and cell apoptosis of podocytes. BBR also significantly reduced PA‑induced ROS production and ER stress in podocytes. These results suggested that PA mediated podocyte apoptosis through enhancing ER stress and the production of ROS. In conclusion, BBR may protect against PA‑induced podocyte apoptosis, and suppression of ROS‑dependent ER stress may be the key mechanism underlying the protective effects of BBR.
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Copy and paste a formatted citation
Spandidos Publications style
Xiang X, Liu T, Wu Y, Jiang X, He J, Chen X and Du X: Berberine alleviates palmitic acid‑induced podocyte apoptosis by reducing reactive oxygen species‑mediated endoplasmic reticulum stress. Mol Med Rep 23: 3, 2021.
APA
Xiang, X., Liu, T., Wu, Y., Jiang, X., He, J., Chen, X., & Du, X. (2021). Berberine alleviates palmitic acid‑induced podocyte apoptosis by reducing reactive oxygen species‑mediated endoplasmic reticulum stress. Molecular Medicine Reports, 23, 3. https://doi.org/10.3892/mmr.2020.11641
MLA
Xiang, X., Liu, T., Wu, Y., Jiang, X., He, J., Chen, X., Du, X."Berberine alleviates palmitic acid‑induced podocyte apoptosis by reducing reactive oxygen species‑mediated endoplasmic reticulum stress". Molecular Medicine Reports 23.1 (2021): 3.
Chicago
Xiang, X., Liu, T., Wu, Y., Jiang, X., He, J., Chen, X., Du, X."Berberine alleviates palmitic acid‑induced podocyte apoptosis by reducing reactive oxygen species‑mediated endoplasmic reticulum stress". Molecular Medicine Reports 23, no. 1 (2021): 3. https://doi.org/10.3892/mmr.2020.11641
Copy and paste a formatted citation
x
Spandidos Publications style
Xiang X, Liu T, Wu Y, Jiang X, He J, Chen X and Du X: Berberine alleviates palmitic acid‑induced podocyte apoptosis by reducing reactive oxygen species‑mediated endoplasmic reticulum stress. Mol Med Rep 23: 3, 2021.
APA
Xiang, X., Liu, T., Wu, Y., Jiang, X., He, J., Chen, X., & Du, X. (2021). Berberine alleviates palmitic acid‑induced podocyte apoptosis by reducing reactive oxygen species‑mediated endoplasmic reticulum stress. Molecular Medicine Reports, 23, 3. https://doi.org/10.3892/mmr.2020.11641
MLA
Xiang, X., Liu, T., Wu, Y., Jiang, X., He, J., Chen, X., Du, X."Berberine alleviates palmitic acid‑induced podocyte apoptosis by reducing reactive oxygen species‑mediated endoplasmic reticulum stress". Molecular Medicine Reports 23.1 (2021): 3.
Chicago
Xiang, X., Liu, T., Wu, Y., Jiang, X., He, J., Chen, X., Du, X."Berberine alleviates palmitic acid‑induced podocyte apoptosis by reducing reactive oxygen species‑mediated endoplasmic reticulum stress". Molecular Medicine Reports 23, no. 1 (2021): 3. https://doi.org/10.3892/mmr.2020.11641
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