Proteins involved in oxidative stress in leiomyoma tissues treated with ulipristal acetate

Ura,Blendi; Monasta,Lorenzo; De Spelorzi,Yeraldin Chiquinquira Castillo; Arrigoni,Giorgio; Franchin,Cinzia; Biffi,Stefania; Aloisio,Michelangelo; Gaita,Bartolomea; Licastro,Danilo; Athanasakis,Emmanouil; Scrimin,Federica; Stabile,Guglielmo; Romano,Federico; Di Lorenzo,Giovanni; Ricci,Giuseppe

doi:10.3892/mmr.2020.11642

Proteins involved in oxidative stress in leiomyoma tissues treated with ulipristal acetate

Authors:
- Blendi Ura
- Lorenzo Monasta
- Yeraldin Chiquinquira Castillo De Spelorzi
- Giorgio Arrigoni
- Cinzia Franchin
- Stefania Biffi
- Michelangelo Aloisio
- Bartolomea Gaita
- Danilo Licastro
- Emmanouil Athanasakis
- Federica Scrimin
- Guglielmo Stabile
- Federico Romano
- Giovanni Di Lorenzo
- Giuseppe Ricci
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Affiliations: Institute for Maternal and Child Health‑IRCCS ‘Burlo Garofolo’, I‑34137 Trieste, Italy, Life Sciences Department, University of Trieste, I‑34128 Trieste, Italy, Department of Biomedical Sciences, University of Padova, I‑35121 Padova, Italy, Central RNA Laboratory, Istituto Italiano di Tecnologia, I‑16163 Genova, Italy, ARGO Open Lab Platform for Genome Sequencing, AREA Science Park, I‑34149 Trieste, Italy
Published online on: November 2, 2020 https://doi.org/10.3892/mmr.2020.11642
Article Number: 4

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Abstract

Uterine leiomyoma presents the highest incidence among benign tumors of the female reproductive tract. The present study compared the proteome of leiomyoma treated with ulipristal acetate with that of untreated leiomyoma to investigate protein expression patterns in relation to oxidative stress. Paired tissue samples from seven treated and untreated leiomyomas were collected and the proteome was analyzed by two‑dimensional gel electrophoresis (2‑DE). Western blotting was used to validate the results of 2‑DE, and mass spectrometry was used to identify proteins. The tissue expression of 30 proteins was markedly affected by treatment with ulipristal acetate. Bioinformatics analysis revealed that several of the differentially expressed proteins were involved in the degradation of hydrogen peroxide and the synthesis of reactive oxygen species. The present study suggested the involvement of oxidative stress as a novel mechanism of action of ulipristal acetate. These findings require further investigations to understand the role of ulipristal acetate in the treatment of the leiomyoma.

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