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miR‑449a‑5p suppresses CDK6 expression to inhibit cardiomyocyte proliferation

  • Authors:
    • Bing Li
    • Zhi Wang
    • Fan Yang
    • Jing Huang
    • Xingwei Hu
    • Shiyan Deng
    • Maobo Tian
    • Xiaoyun Si
  • View Affiliations / Copyright

    Affiliations: School of Medicine, Guizhou University, Guiyang, Guizhou 550025, P.R. China, Department of Emergency Medicine, Qingdao Municipal Hospital (Group), Qingdao, Shandong 266011, P.R. China, Department of Cardiology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, P.R. China, Department of Cardiology, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 14
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    Published online on: November 3, 2020
       https://doi.org/10.3892/mmr.2020.11652
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Abstract

Induction of cardiomyocyte (CM) proliferation is a promising approach for cardiac regeneration following myocardial injury. MicroRNAs (miRs) have been reported to regulate CM proliferation. In particular, miR‑449a‑5p has been identified to be associated with CM proliferation in previous high throughput functional screening data. However, whether miR‑449a‑5p regulates CM proliferation has not been thoroughly investigated. This study aimed to explore whether miR‑449a‑5p modulates CM proliferation and to identify the molecular mechanism via which miR‑449a‑5p regulates CM proliferation. The current study demonstrated that miR‑449a‑5p expression levels were significantly increased during heart development. Furthermore, the results suggested that miR‑449a‑5p mimic inhibited CM proliferation in vitro as determined via immunofluorescence for ki67 and histone H3 phosphorylated at serine 10 (pH3), as well as the numbers of CMs. However, miR‑449a‑5p knockdown promoted CM proliferation. CDK6 was identified as a direct target gene of miR‑449a‑5p, and CDK6 mRNA and protein expression was suppressed by miR‑449a‑5p. Moreover, CDK6 gain‑of‑function increased CM proliferation. Overexpression of CDK6 also blocked the inhibitory effect of miR‑449a‑5p on CM proliferation, indicating that CDK6 was a functional target of miR‑449a‑5p in CM proliferation. In conclusion, miR‑449a‑5p inhibited CM proliferation by targeting CDK6, which provides a potential molecular target for preventing myocardial injury.
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Copy and paste a formatted citation
Spandidos Publications style
Li B, Wang Z, Yang F, Huang J, Hu X, Deng S, Tian M and Si X: miR‑449a‑5p suppresses CDK6 expression to inhibit cardiomyocyte proliferation. Mol Med Rep 23: 14, 2021.
APA
Li, B., Wang, Z., Yang, F., Huang, J., Hu, X., Deng, S. ... Si, X. (2021). miR‑449a‑5p suppresses CDK6 expression to inhibit cardiomyocyte proliferation. Molecular Medicine Reports, 23, 14. https://doi.org/10.3892/mmr.2020.11652
MLA
Li, B., Wang, Z., Yang, F., Huang, J., Hu, X., Deng, S., Tian, M., Si, X."miR‑449a‑5p suppresses CDK6 expression to inhibit cardiomyocyte proliferation". Molecular Medicine Reports 23.1 (2021): 14.
Chicago
Li, B., Wang, Z., Yang, F., Huang, J., Hu, X., Deng, S., Tian, M., Si, X."miR‑449a‑5p suppresses CDK6 expression to inhibit cardiomyocyte proliferation". Molecular Medicine Reports 23, no. 1 (2021): 14. https://doi.org/10.3892/mmr.2020.11652
Copy and paste a formatted citation
x
Spandidos Publications style
Li B, Wang Z, Yang F, Huang J, Hu X, Deng S, Tian M and Si X: miR‑449a‑5p suppresses CDK6 expression to inhibit cardiomyocyte proliferation. Mol Med Rep 23: 14, 2021.
APA
Li, B., Wang, Z., Yang, F., Huang, J., Hu, X., Deng, S. ... Si, X. (2021). miR‑449a‑5p suppresses CDK6 expression to inhibit cardiomyocyte proliferation. Molecular Medicine Reports, 23, 14. https://doi.org/10.3892/mmr.2020.11652
MLA
Li, B., Wang, Z., Yang, F., Huang, J., Hu, X., Deng, S., Tian, M., Si, X."miR‑449a‑5p suppresses CDK6 expression to inhibit cardiomyocyte proliferation". Molecular Medicine Reports 23.1 (2021): 14.
Chicago
Li, B., Wang, Z., Yang, F., Huang, J., Hu, X., Deng, S., Tian, M., Si, X."miR‑449a‑5p suppresses CDK6 expression to inhibit cardiomyocyte proliferation". Molecular Medicine Reports 23, no. 1 (2021): 14. https://doi.org/10.3892/mmr.2020.11652
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