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A transcriptome profile in gallbladder cancer based on annotation analysis of microarray studies

  • Authors:
    • Chunlin Ge
    • Xuan Zhu
    • Xing Niu
    • Bingye Zhang
    • Lijie Chen
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Second Clinical College, Shengjing Hospital Affiliated to China Medical University, Shenyang, Liaoning 110004, P.R. China
    Copyright: © Ge et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 25
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    Published online on: November 3, 2020
       https://doi.org/10.3892/mmr.2020.11663
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Abstract

The purpose of the present study was to identify aberrantly expressed genes for gallbladder cancer based on the annotation analysis of microarray studies and to explore their potential functions. Differential gene expression was investigated in cholesterol polyps, gallbladder adenoma and gallbladder cancer using microarrays. Subsequently, microarray results were comprehensively analyzed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to determine the affected biological processes or pathways. Differentially expressed genes (DEGs) of cholesterol polyps, gallbladder adenoma and gallbladder cancer were identified. Following comprehensive analysis, 14 genes were found to be differentially expressed in the gallbladder wall of both gallbladder cancer and gallbladder adenoma. The 20 most significantly upregulated genes were only upregulated in the gallbladder wall of gallbladder cancer, but not in the gallbladder wall of cholesterol polyps and gallbladder adenoma. In addition, 182 DEGs were upregulated in the gallbladder wall of gallbladder adenoma compared with the gallbladder wall of cholesterol polyps. A total of 20 most significant DEGs were found in both the tumor and gallbladder wall of gallbladder cancer. In addition, the most significant DEGs that were identified were only upregulated in the tumor of gallbladder cancer. GO and KEGG analysis indicated that the aforementioned DEGs could participate in numerous biological processes or pathways associated with the development of gallbladder cancer. The present findings will help improve the current understanding of tumorigenesis and the development of gallbladder cancer.
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1 

Sicklick JK, Fanta PT, Shimabukuro K and Kurzrock R: Genomics of gallbladder cancer: The case for biomarker-driven clinical trial design. Cancer Metastasis Rev. 35:263–275. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Chandra V, Kim JJ, Mittal B and Rai R: MicroRNA aberrations: An emerging field for gallbladder cancer management. World J Gastroenterol. 22:1787–1799. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Kim YT, Kim J, Jang YH, Lee WJ, Ryu JK, Park YK, Kim SW, Kim WH, Yoon YB and Kim CY: Genetic alterations in gallbladder adenoma, dysplasia and carcinoma. Cancer Lett. 169:59–68. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Itoi T, Watanabe H, Ajioka Y, Oohashi Y, Takel K, Nishikura K, Nakamura Y, Horil A and Saito T: APC, K-ras codon 12 mutations and p53 gene expression in carcinoma and adenoma of the gall-bladder suggest two genetic pathways in gallbladder carcinogenesis. Pathol Int. 46:333–340. 1996. View Article : Google Scholar : PubMed/NCBI

5 

Lee SH, Lee DS, You IY, Jeon WJ, Park SM, Youn SJ, Choi JW and Sung R: Histopathologic analysis of adenoma and adenoma-related lesions of the gallbladder. Korean J Gastroenterol. 55:119–126. 2010.(In Korean). View Article : Google Scholar : PubMed/NCBI

6 

Liu MC, Jiang L, Hong HJ, Meng ZW, Du Q, Zhou LY, She FF and Chen YL: Serum vascular endothelial growth factors C and D as forecast tools for patients with gallbladder carcinoma. Tumour Biol. 36:6305–6312. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Choi JH, Yun JW, Kim YS, Lee EA, Hwang ST, Cho YK, Kim HJ, Park JH, Park DI, Sohn CI, et al: Pre-operative predictive factors for gallbladder cholesterol polyps using conventional diagnostic imaging. World J Gastroenterol. 14:6831–6834. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Zhao MF, Huang P, Ge CL, Sun T, Ma ZG and Ye FF: Conjugated bile acids in gallbladder bile and serum as potential biomarkers for cholesterol polyps and adenomatous polyps. Int J Biol Markers. 31:e73–e79. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Ritchie ME, Phipson B, Wu D, Hu Y, Law CW, Shi W and Smyth GK: limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res. 43:e472015. View Article : Google Scholar : PubMed/NCBI

10 

Subramanian A, Tamayo P, Mootha VK, Mukherjee S, Ebert BL, Gillette MA, Paulovich A, Pomeroy SL, Golub TR, Lander ES and Mesirov JP: Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci USA. 102:15545–15550. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Kanehisa M, Goto S, Sato Y, Furumichi M and Tanabe M: KEGG for integration and interpretation of large-scale molecular data sets. Nucleic Acids Res. 40((Database Issue)): D109–D114. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Tang Z, Li C, Kang B, Gao G, Li C and Zhang Z: GEPIA: A web server for cancer and normal gene expression profiling and interactive analyses. Nucleic Acids Res. 45W:W98–W102. 2017. View Article : Google Scholar

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14 

R Core Team: R: A language and environment for statistical computing. R Foundation for Statistical Computing; Vienna, Austria: 2013, simplehttp://www.R-project.org/

15 

Rodriguez S, Gaunt TR, Guo Y, Zheng J, Barnes MR, Tang W, Danish F, Johnson A, Castillo BA, Li YR, et al: Lipids, obesity and gallbladder disease in women: Insights from genetic studies using the cardiovascular gene-centric 50K SNP array. Eur J Hum Genet. 24:106–112. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Mellnick VM, Menias CO, Sandrasegaran K, Hara AK, Kielar AZ, Brunt EM, Doyle MB, Dahiya N and Elsayes KM: Polypoid lesions of the gallbladder: Disease spectrum with pathologic correlation. Radiographics. 5:387–399. 2015. View Article : Google Scholar

17 

Wang F, Wang R, Li Q, Qu X, Hao Y, Yang J, Zhao H, Wang Q, Li G, Zhang F, et al: A transcriptome profile in hepatocellular carcinomas based on integrated analysis of microarray studies. Diagn Pathol. 12:42017. View Article : Google Scholar : PubMed/NCBI

18 

Meng LZ, Fang JG, Sun JW, Yang F and Wei YX: Aberrant expression profile of long noncoding RNA in human sinonasal squamous cell carcinoma by microarray analysis. Biomed Res Int. 2016:10957102016. View Article : Google Scholar : PubMed/NCBI

19 

Liu L, Liu Y, Liu C, Zhang Z, Du Y and Zhao H: Analysis of gene expression profile identifies potential biomarkers for atherosclerosis. Mol Med Rep. 14:3052–3058. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Park H, Jung HY, Choi HJ, Kim DY, Yoo JY, Yun CO, Min JK, Kim YM and Kwon YG: Distinct roles of DKK1 and DKK2 in tumor angiogenesis. Angiogenesis. 17:221–234. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Ma Z, Li W, Yoshiya S, Xu Y, Hata M, El-Darawish Y, Markova T, Yamanishi K, Yamanishi H, Tahara H, et al: Augmentation of immune checkpoint cancer immunotherapy with IL18. Clin Cancer Res. 22:2969–2980. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Roongta UV, Pabalan JG, Wang X, Ryseck RP, Fargnoli J, Henley BJ, Yang WP, Zhu J, Madireddi MT, Lawrence RM, et al: Cancer cell dependence on unsaturated fatty acids implicates stearoyl-CoA desaturase as a target for cancer therapy. Mol Cancer Res. 9:1551–1561. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Yin D, Komatsu N, Miller CW, Chumakov AM, Marschesky A, McKenna R, Black KL and Koeffler HP: KLF6: mutational analysis and effect on cancer cell proliferation. Int J Oncol. 30:65–72. 2007.PubMed/NCBI

24 

Grünberg JR, Elvin J, Paul A, Hedjazifar S, Hammarstedt A and Smith U: CCN5/WISP2 and metabolic diseases. J Cell Commun Signal. 12:309–318. 2018. View Article : Google Scholar : PubMed/NCBI

25 

Chang XL, Jin TY and Zhou YF: Metallothionein 1 isoform gene expression induced by cadmium in human peripheral blood lymphocytes. Biomed Environ Sci. 19:104–109. 2006.PubMed/NCBI

26 

Lee MH, Gao YT, Huang YH, McGee EE, Lam T, Wang B, Shen MC, Rashid A, Pfeiffer RM, Hsing AW and Koshiol J: A metallomic approach to assess associations of serum metal levels with gallstones and gallbladder cancer. Hepatology. 71:917–928. 2020. View Article : Google Scholar : PubMed/NCBI

27 

En-lin S, Sheng-guo C and Hua-qiao W: The expression of EFEMP1 in cervical carcinoma and its relationship with prognosis. Gynecol Oncol. 117:417–422. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Habtewold A, Amogne W, Makonnen E, Yimer G, Riedel KD, Ueda N, Worku A, Haefeli WE, Lindquist L, Aderaye G, et al: Long-term effect of efavirenz autoinduction on plasma/peripheral blood mononuclear cell drug exposure and CD4 count is influenced by UGT2B7 and CYP2B6 genotypes among HIV patients. J Antimicrob Chemother. 66:2350–2361. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Zhang C, Lu L, Li Y, Wang X, Zhou J, Liu Y, Fu P, Gallicchio MA, Bach LA and Duan C: IGF binding protein-6 expression in vascular endothelial cells is induced by hypoxia and plays a negative role in tumor angiogenesis. Int J Cancer. 130:2003–2012. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Liu W and Rodgers GP: Olfactomedin 4 expression and functions in innate immunity, inflammation, and cancer. Cancer Metastasis Rev. 35:201–212. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Dey A, Parmar D, Dayal M, Dhawan A and Seth PK: Cytochrome P450 1A1 (CYP1A1) in blood lymphocytes evidence for catalytic activity and mRNA expression. Life Sci. 69:383–393. 2001. View Article : Google Scholar : PubMed/NCBI

32 

Podestà M, Benvenuto F, Pitto A, Figari O, Bacigalupo A, Bruzzone S, Guida L, Franco L, Paleari L, Bodrato N, et al: Concentrative uptake of cyclic ADP-ribose generated by BST-1+ stroma stimulates proliferation of human hematopoietic progenitors. J Biol Chem. 280:5343–5349. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Osugi Y, Vuckovic S and Hart DN: Myeloid blood CD11c(+) dendritic cells and monocyte-derived dendritic cells differ in their ability to stimulate T lymphocytes. Blood. 100:2858–2866. 2002. View Article : Google Scholar : PubMed/NCBI

34 

Vallvé JC, Serra N, Zalba G, Fortuño A, Beloqui O, Ferre R, Ribalta J and Masana L: Two variants in the fibulin2 gene are associated with lower systolic blood pressure and decreased risk of hypertension. PLoS One. 7:e430512012. View Article : Google Scholar : PubMed/NCBI

35 

Gloury R, Zotos D, Zuidscherwoude M, Masson F, Liao Y, Hasbold J, Corcoran LM, Hodgkin PD, Belz GT, Shi W, et al: Dynamic changes in Id3 and E-protein activity orchestrate germinal center and plasma cell development. J Exp Med. 213:1095–1111. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ge C, Zhu X, Niu X, Zhang B and Chen L: A transcriptome profile in gallbladder cancer based on annotation analysis of microarray studies. Mol Med Rep 23: 25, 2021.
APA
Ge, C., Zhu, X., Niu, X., Zhang, B., & Chen, L. (2021). A transcriptome profile in gallbladder cancer based on annotation analysis of microarray studies. Molecular Medicine Reports, 23, 25. https://doi.org/10.3892/mmr.2020.11663
MLA
Ge, C., Zhu, X., Niu, X., Zhang, B., Chen, L."A transcriptome profile in gallbladder cancer based on annotation analysis of microarray studies". Molecular Medicine Reports 23.1 (2021): 25.
Chicago
Ge, C., Zhu, X., Niu, X., Zhang, B., Chen, L."A transcriptome profile in gallbladder cancer based on annotation analysis of microarray studies". Molecular Medicine Reports 23, no. 1 (2021): 25. https://doi.org/10.3892/mmr.2020.11663
Copy and paste a formatted citation
x
Spandidos Publications style
Ge C, Zhu X, Niu X, Zhang B and Chen L: A transcriptome profile in gallbladder cancer based on annotation analysis of microarray studies. Mol Med Rep 23: 25, 2021.
APA
Ge, C., Zhu, X., Niu, X., Zhang, B., & Chen, L. (2021). A transcriptome profile in gallbladder cancer based on annotation analysis of microarray studies. Molecular Medicine Reports, 23, 25. https://doi.org/10.3892/mmr.2020.11663
MLA
Ge, C., Zhu, X., Niu, X., Zhang, B., Chen, L."A transcriptome profile in gallbladder cancer based on annotation analysis of microarray studies". Molecular Medicine Reports 23.1 (2021): 25.
Chicago
Ge, C., Zhu, X., Niu, X., Zhang, B., Chen, L."A transcriptome profile in gallbladder cancer based on annotation analysis of microarray studies". Molecular Medicine Reports 23, no. 1 (2021): 25. https://doi.org/10.3892/mmr.2020.11663
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