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Article

Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells

  • Authors:
    • Ye-Rang Yun
    • Jun-Hyeog Jang
  • View Affiliations / Copyright

    Affiliations: Industrial Technology Research Group, Research and Development Division, World Institute of Kimchi, Nam, Gwangju 61755, Republic of Korea, Department of Biochemistry, Inha University School of Medicine, Incheon 22212, Republic of Korea
  • Article Number: 61
    |
    Published online on: November 18, 2020
       https://doi.org/10.3892/mmr.2020.11699
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Abstract

Stromal cell‑derived factor‑1 (SDF‑1) is a chemokine involved in neuronal differentiation, as well as proliferation and migration. In the present study, the effects of recombinant SDF‑1 on neurite outgrowth for nerve regeneration and engineering were evaluated in PC‑12 cells. The effects of purified SDF‑1 protein on cell toxicity, proliferation and migration were also assessed. SDF‑1 significantly augmented cell proliferation in a dose‑dependent manner, with low cytotoxicity in PC‑12 cells. Cell migration also increased in the presence of SDF‑1. SDF‑1 significantly increased neurite number and length, compared with the control (untreated cells). Neurofilament mRNA levels, which are involved in neuronal differentiation, were also significantly upregulated in the presence of SDF‑1. These results suggested that SDF‑1 might prove useful for tissue engineering through induction of neuronal differentiation.
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1 

Guyon A: CXCL12 chemokine and its receptors as major players in the interactions between immune and nervous systems. Front Cell Neurosci. 8:652014. View Article : Google Scholar : PubMed/NCBI

2 

Takano T, Li YJ, Kukita A, Yamaza T, Ayukawa Y, Moriyama K, Uehara N, Nomiyama H, Koyano K and Kukita T: Mesenchymal stem cells markedly suppress inflammatory bone destruction in rats with adjuvant-induced arthritis. Lab Invest. 94:286–296. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Hu Y, Lu J, Xu X, Lyu J and Zhang H: Regulation of focal adhesion turnover in SDF-1α-stimulated migration of mesenchymal stem cells in neural differentiation. Sci Rep. 7:100132017. View Article : Google Scholar : PubMed/NCBI

4 

Zhu H, Wang X, Han Y, Zhang W, Xin W, Zheng X and Zhang J: Icariin promotes the migration of bone marrow stromal cells via the SDF-1α/HIF-1α/CXCR4 pathway. Drug Des Devel Ther. 12:4023–4031. 2018. View Article : Google Scholar : PubMed/NCBI

5 

Chin S, Furukawa KI, Kurotaki K, Nagasaki S, Wada K, Kumagai G, Motomura S and Ishibashi Y: Facilitation of chemotaxis activity of mesenchymal stem cells via stromal cell-derived factor-1 and its receptor may promote ectopic ossification of human spinal ligaments. J Pharmacol Exp Ther. 369:1–8. 2019. View Article : Google Scholar : PubMed/NCBI

6 

Shyu WC, Lin SZ, Yen PS, Su CY, Chen DC, Wang HJ and Li H: Stromal cell-derived factor-1 alpha promotes neuroprotection, angiogenesis, and mobilization/homing of bone marrow-derived cells in stroke rats. J Pharmacol Exp Ther. 324:834–849. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Xu JT, Qian Y, Wang W, Chen XX, Li Y, Li Y, Yang ZY, Song XB, Lu D and Deng XL: Effect of stromal cell-derived factor-1/CXCR4 axis in neural stem cell transplantation for Parkinson's disease. Neural Regen Res. 15:112–119. 2020. View Article : Google Scholar : PubMed/NCBI

8 

Addington CP, Pauken CM, Caplan MR and Stabenfeldt SE: The role of SDF-1α-ECM crosstalk in determining neural stem cell fate. Biomaterials. 35:3263–3272. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Kim SY, Park SH and Shin JW, Kang YG, Jeon KJ, Hyun JS, Oh MJ and Shin JW: Mechanical stimulation and the presence of neighboring cells greatly affect migration of human mesenchymal stem cells. Biotechnol Lett. 35:1817–1822. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Hu Y, Chen W, Wu L, Jiang L, Qin H and Tang N: Hypoxic preconditioning improves the survival and neural effects of transplanted mesenchymal stem cells via CXCL12/CXCR4 signalling in a rat model of cerebral infarction. Cell Biochem Funct. 37:504–515. 2019. View Article : Google Scholar : PubMed/NCBI

11 

Sheu ML, Cheng FC, Su HL, Chen YJ, Chen CJ, Chiang CM, Chiu WT, Sheehan J and Pan HC: Recruitment by SDF-1α of CD34-positive cells involved in sciatic nerve regeneration. J Neurosurg. 116:432–444. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Lindenbaum MH, Carbonetto S, Grosveld F, Flavell D and Mushynski WE: Transcriptional and post-transcriptional effects of nerve growth factor on expression of the three neurofilament subunits in PC-12 cells. J Biol Chem. 263:5662–5667. 1988.PubMed/NCBI

13 

Seow SLS, Hong SL, Lee GS, Malek SNA and Sabaratnam V: 6-shogaol, a neuroactive compound of ginger (jahe gajah) induced neuritogenic activity via NGF responsive pathways in PC-12 cells. BMC Complement Altern Med. 17:3342017. View Article : Google Scholar : PubMed/NCBI

14 

Mazzio E, Georges B, McTier O and Soliman KF: Neurotrophic effects of Mu Bie Zi (Momordica cochinchinensis) seed elucidated by high-throughput screening of natural products for NGF mimetic effects in PC-12 cells. Neurochem Res. 40:2102–2112. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Chen JH, Lee DC and Chiu IM: Cytotoxic effects of acrylamide in nerve growth factor or fibroblast growth factor 1-induced neurite outgrowth in PC12 cells. Arch Toxicol. 88:769–780. 2014.PubMed/NCBI

16 

Vergaño-Vera E, Díaz-Guerra E, Rodríguez-Traver E, Méndez-Gómez HR, Solís Ó, Pignatelli J, Pickel J, Lee SH, Moratalla R and Vicario-Abejón C: Nurr1 blocks the mitogenic effect of FGF-2 and EGF, inducing olfactory bulb neural stem cells to adopt dopaminergic and dopaminergic-GABAergic neuronal phenotypes. Dev Neurobiol. 75:823–841. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Dashnyam K, Perez R, Lee EJ, Yun YR, Jang JH, Wall IB and Kim HW: Hybrid scaffolds of gelatin-siloxane releasing stromal derived factor-1 effective for cell recruitment. J Biomed Mater Res A. 102:1859–1867. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Yun YR, Lee S, Jeon E, Kang W, Kim KH, Kim HW and Jang JH: Fibroblast growth factor 2-functionalized collagen matrices for skeletal muscle tissue engineering. Biotechnol Lett. 34:771–778. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Sun J, Mou C, Shi Q, Chen B, Hou X, Zhang W, Li X, Zhuang Y, Shi J, Chen Y, et al: Controlled release of collagen-binding SDF-1α from the collagen scaffold promoted tendon regeneration in a rat Achilles tendon defect model. Biomaterials. 162:22–33. 2018. View Article : Google Scholar : PubMed/NCBI

21 

Tang Q, Luo C, Lu B, Fu Q, Yin H, Qin Z, Lyu D, Zhang L, Fang Z, Zhu Y, et al: Thermosensitive chitosan-based hydrogels releasing stromal cell derived factor-1 alpha recruit MSC for corneal epithelium regeneration. Acta Biomater. 61:101–113. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Li Z, Zhao R, Fang X, Huang Q and Liu J: Recombinant human SDF-1α administration accelerates aneurysm neck reendothelialization in rabbit saccular aneurysm after flow diverter treatment. Acta Biochim Biophys Sin (Shanghai). 49:246–253. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Jo HS, Yeo EJ, Shin MJ, Choi YJ, Yeo HJ, Cho SB, Park JH, Lee CH, Eum WS and Choi SY: Tat-DJ-1 enhances cell survival by inhibition of oxidative stress, NF-κB and MAPK activation in HepG2 cells. Biotechnol Lett. 39:511–521. 2017. View Article : Google Scholar : PubMed/NCBI

24 

Yun YR, Jeon E, Lee S, Kang W, Kim SG, Kim HW, Suh CK and Jang JH: Expression, purification, and characterization of a dentin phosphoprotein produced by Escherichia coli, and its odontoblastic differentiation effects on human dental pulp cells. Protein J. 31:504–510. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Li Y, Chang S, Li W, Tang G, Ma Y, Liu Y, Yuan F, Zhang Z, Yang GY and Wang Y: cxcl12-engineered endothelial progenitor cells enhance neurogenesis and angiogenesis after ischemic brain injury in mice. Stem Cell Res Ther. 9:1392018. View Article : Google Scholar : PubMed/NCBI

26 

Chen Y, Wu T, Huang S, Suen CW, Cheng X, Li J, Hou H, She G, Zhang H, Wang H, et al: Sustained release SDF-1α/TGF-β1-loaded silk fibroin-porous gelatin scaffold promotes cartilage repair. ACS Appl Mater Interfaces. 11:14608–14618. 2019. View Article : Google Scholar : PubMed/NCBI

27 

Eik LF, Naidu M, David P, Wong KH, Tan YS and Sabaratnam V: Lignosus rhinocerus (Cooke) ryvarden: A medicinal mushroom that stimulates neurite outgrowth in PC-12 Cells. Evid Based Complement Alternat Med. 2012:3203082012. View Article : Google Scholar : PubMed/NCBI

28 

Lam CT, Gong AG, Lam KY, Zhang LM, Chen JP, Dong TT, Lin HQ and Tsim KW: Jujube-containing herbal decoctions induce neuronal differentiation and the expression of anti-oxidant enzymes in cultured PC12 cells. J Ethnopharmacol. 188:275–283. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Chen J, Maiwulanjiang M, Lam KY, Zhang WL, Zhan JY, Lam CT, Xu SL, Zhu KY, Yao P, Lau DT, et al: A standardized extract of the fruit of Ziziphus jujuba (Jujube) induces neuronal differentiation of cultured PC12 cells: A signaling mediated by protein kinase A. J Agric Food Chem. 62:1890–1897. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Murphy A, Breen KC, Long A, Feighery C, Casey EB and Kelleher D: Neurofilament expression in human T lymphocytes. Immunology. 79:167–170. 1993.PubMed/NCBI

31 

Srivastava A, Singh S, Pandey A, Kumar D, Rajpurohit CS, Khanna VK and Pant AB: Secretome of differentiated PC12 cells enhances neuronal differentiation in human mesenchymal stem cells via NGF-like mechanism. Mol Neurobiol. 55:8293–8305. 2018. View Article : Google Scholar : PubMed/NCBI

32 

Yan L, Xu SL, Zhu KY, Lam KY, Xin G, Maiwulanjiang M, Li N, Dong TT, Lin H and Tsim KW: Optimizing the compatibility of paired-herb in an ancient Chinese herbal decoction Kai-Xin-San in activating neurofilament expression in cultured PC12 cells. J Ethnopharmacol. 162:155–162. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Ernsberger U, Esposito L, Partimo S, Huber K, Franke A, Bixby JL, Kalcheim C and Unsicker K: Expression of neuronal markers suggests heterogeneity of chick sympathoadrenal cells prior to invasion of the adrenal anlagen. Cell Tissue Res. 319:1–13. 2005. View Article : Google Scholar : PubMed/NCBI

34 

Vaudry D, Stork PJS, Lazarovici P and Eiden LE: Signaling pathways for PC12 cell differentiation: Making the right connections. Science. 296:1648–1649. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Yao R, Yoshihara M and Osada H: Specific activation of a c-Jun NH2-terminal kinase isoform and induction of neurite outgrowth in PC-12 cells by staurosporine. J Biol Chem. 272:18261–18266. 1997. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yun Y and Jang J: Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells. Mol Med Rep 23: 61, 2021.
APA
Yun, Y., & Jang, J. (2021). Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells. Molecular Medicine Reports, 23, 61. https://doi.org/10.3892/mmr.2020.11699
MLA
Yun, Y., Jang, J."Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells". Molecular Medicine Reports 23.1 (2021): 61.
Chicago
Yun, Y., Jang, J."Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells". Molecular Medicine Reports 23, no. 1 (2021): 61. https://doi.org/10.3892/mmr.2020.11699
Copy and paste a formatted citation
x
Spandidos Publications style
Yun Y and Jang J: Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells. Mol Med Rep 23: 61, 2021.
APA
Yun, Y., & Jang, J. (2021). Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells. Molecular Medicine Reports, 23, 61. https://doi.org/10.3892/mmr.2020.11699
MLA
Yun, Y., Jang, J."Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells". Molecular Medicine Reports 23.1 (2021): 61.
Chicago
Yun, Y., Jang, J."Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells". Molecular Medicine Reports 23, no. 1 (2021): 61. https://doi.org/10.3892/mmr.2020.11699
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