Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells

  • Authors:
    • Ye-Rang Yun
    • Jun-Hyeog Jang
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  • Published online on: November 18, 2020     https://doi.org/10.3892/mmr.2020.11699
  • Article Number: 61
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Abstract

Stromal cell‑derived factor‑1 (SDF‑1) is a chemokine involved in neuronal differentiation, as well as proliferation and migration. In the present study, the effects of recombinant SDF‑1 on neurite outgrowth for nerve regeneration and engineering were evaluated in PC‑12 cells. The effects of purified SDF‑1 protein on cell toxicity, proliferation and migration were also assessed. SDF‑1 significantly augmented cell proliferation in a dose‑dependent manner, with low cytotoxicity in PC‑12 cells. Cell migration also increased in the presence of SDF‑1. SDF‑1 significantly increased neurite number and length, compared with the control (untreated cells). Neurofilament mRNA levels, which are involved in neuronal differentiation, were also significantly upregulated in the presence of SDF‑1. These results suggested that SDF‑1 might prove useful for tissue engineering through induction of neuronal differentiation.
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January-2021
Volume 23 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yun Y and Yun Y: Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells. Mol Med Rep 23: 61, 2021
APA
Yun, Y., & Yun, Y. (2021). Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells. Molecular Medicine Reports, 23, 61. https://doi.org/10.3892/mmr.2020.11699
MLA
Yun, Y., Jang, J."Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells". Molecular Medicine Reports 23.1 (2021): 61.
Chicago
Yun, Y., Jang, J."Recombinant stromal cell‑derived factor‑1 protein promotes neurite outgrowth in PC‑12 cells". Molecular Medicine Reports 23, no. 1 (2021): 61. https://doi.org/10.3892/mmr.2020.11699