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Article

Nardostachin from Nardostachys jatamansi exerts anti‑neuroinflammatory effects through TLR4/MyD88‑related suppression of the NF‑κB and JNK MAPK signaling pathways in lipopolysaccharide‑induced BV2 and primary microglial cells

  • Authors:
    • Dong-Cheol Kim
    • Jin-Soo Park
    • Chi-Su Yoon
    • Youn-Chul Kim
    • Hyuncheol Oh
  • View Affiliations / Copyright

    Affiliations: College of Pharmacy, Wonkwang University, Iksan, Jeollabuk‑do 54538, Republic of Korea, Korea Research Institute of Bioscience and Biotechnology, Yeongudanji‑ro, Cheongwon 28116, Republic of Korea
  • Article Number: 82
    |
    Published online on: November 23, 2020
       https://doi.org/10.3892/mmr.2020.11720
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Abstract

Through searching for anti‑neuroinflammatory metabolites from Nardostachys jatamansi extracts, nardostachin was revealed to exert anti‑neuroinflammatory effects against lipopolysaccharide (LPS)‑induced overproduction of nitric oxide and prostaglandin E2 in BV2 and rat primary microglial cells. Furthermore, nardostachin inhibited the production of inducible nitric oxide synthase and cyclooxygenase‑2 as well as pro‑inflammatory cytokines, including interleukin (IL)‑1β, IL‑6, IL‑12 and tumor necrosis factor‑α in LPS‑stimulated BV2 and rat primary microglial cells. In a mechanistic study, nardostachin exhibited inhibitory activity on the nuclear factor (NF)‑κB signaling pathway in LPS‑stimulated BV2 and rat primary microglial cells by repressing IκB‑α phosphorylation and blocking NF‑κB translocation. Furthermore, nardostachin exhibited inhibitory effects on LPS‑induced phosphorylation of c‑Jun N‑terminal kinase (JNK) mitogen‑activated protein kinase (MAPK). Additionally, nardostachin repressed protein expression of Toll‑like receptor 4 (TLR4) and myeloid differentiation factor 88 (MyD88) in LPS‑induced BV2 and rat primary microglial cells. These results suggested that nardostachin exerts anti‑neuroinflammatory effects on LPS‑induced BV2 and rat primary microglial cells by suppressing the TLR4‑MyD88‑NF‑κB and JNK MAPK pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Kim D, Park J, Yoon C, Kim Y and Oh H: Nardostachin from <em>Nardostachys jatamansi</em> exerts anti‑neuroinflammatory effects through TLR4/MyD88‑related suppression of the NF‑κB and JNK MAPK signaling pathways in lipopolysaccharide‑induced BV2 and primary microglial cells. Mol Med Rep 23: 82, 2021.
APA
Kim, D., Park, J., Yoon, C., Kim, Y., & Oh, H. (2021). Nardostachin from <em>Nardostachys jatamansi</em> exerts anti‑neuroinflammatory effects through TLR4/MyD88‑related suppression of the NF‑κB and JNK MAPK signaling pathways in lipopolysaccharide‑induced BV2 and primary microglial cells. Molecular Medicine Reports, 23, 82. https://doi.org/10.3892/mmr.2020.11720
MLA
Kim, D., Park, J., Yoon, C., Kim, Y., Oh, H."Nardostachin from <em>Nardostachys jatamansi</em> exerts anti‑neuroinflammatory effects through TLR4/MyD88‑related suppression of the NF‑κB and JNK MAPK signaling pathways in lipopolysaccharide‑induced BV2 and primary microglial cells". Molecular Medicine Reports 23.1 (2021): 82.
Chicago
Kim, D., Park, J., Yoon, C., Kim, Y., Oh, H."Nardostachin from <em>Nardostachys jatamansi</em> exerts anti‑neuroinflammatory effects through TLR4/MyD88‑related suppression of the NF‑κB and JNK MAPK signaling pathways in lipopolysaccharide‑induced BV2 and primary microglial cells". Molecular Medicine Reports 23, no. 1 (2021): 82. https://doi.org/10.3892/mmr.2020.11720
Copy and paste a formatted citation
x
Spandidos Publications style
Kim D, Park J, Yoon C, Kim Y and Oh H: Nardostachin from <em>Nardostachys jatamansi</em> exerts anti‑neuroinflammatory effects through TLR4/MyD88‑related suppression of the NF‑κB and JNK MAPK signaling pathways in lipopolysaccharide‑induced BV2 and primary microglial cells. Mol Med Rep 23: 82, 2021.
APA
Kim, D., Park, J., Yoon, C., Kim, Y., & Oh, H. (2021). Nardostachin from <em>Nardostachys jatamansi</em> exerts anti‑neuroinflammatory effects through TLR4/MyD88‑related suppression of the NF‑κB and JNK MAPK signaling pathways in lipopolysaccharide‑induced BV2 and primary microglial cells. Molecular Medicine Reports, 23, 82. https://doi.org/10.3892/mmr.2020.11720
MLA
Kim, D., Park, J., Yoon, C., Kim, Y., Oh, H."Nardostachin from <em>Nardostachys jatamansi</em> exerts anti‑neuroinflammatory effects through TLR4/MyD88‑related suppression of the NF‑κB and JNK MAPK signaling pathways in lipopolysaccharide‑induced BV2 and primary microglial cells". Molecular Medicine Reports 23.1 (2021): 82.
Chicago
Kim, D., Park, J., Yoon, C., Kim, Y., Oh, H."Nardostachin from <em>Nardostachys jatamansi</em> exerts anti‑neuroinflammatory effects through TLR4/MyD88‑related suppression of the NF‑κB and JNK MAPK signaling pathways in lipopolysaccharide‑induced BV2 and primary microglial cells". Molecular Medicine Reports 23, no. 1 (2021): 82. https://doi.org/10.3892/mmr.2020.11720
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