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Article

Inflammasome components and ADAMTS4 in premature rupture of membranes

  • Authors:
    • Jinming Zhu
    • Chunling Ma
    • Xiaomei Luan
    • Juan Li
    • Fengyun Peng
    • Lei Huang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics, Xuzhou Maternity and Child Health Care Hospital Affiliated to Xuzhou Medical University, Xuzhou, Jiangsu 221009, P.R. China, Graduate School, Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China
  • Article Number: 101
    |
    Published online on: December 1, 2020
       https://doi.org/10.3892/mmr.2020.11740
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Abstract

Inflammation may be responsible for the development of premature rupture of membranes (PROM) including preterm PROM (PPROM) and mature PROM (MPROM). A total of four classic receptor proteins have been confirmed to assemble inflammasomes: NLR family pyrin domain containing (NLRP)1, NLRP3 and NLR family CARD‑domain containing 4 (NLRC4) and absent in melanoma 2 (AIM2). The activation and expression of these receptor‑modulated inflammasomes in placenta and fetal membrane of PROM pregnancies requires investigation. In addition, a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4) is a risk factor for PROM, but whether its expression is associated with inflammasome activation remains to be elucidated. In the present study, the placenta and fetal membrane tissues of patients who had suffered PPROM and MPROM and healthy pregnancies were investigated. Reverse transcription‑quantitative PCR was used to determine the mRNA expression of inflammasomes and ADAMTS4. Western blotting, immunohistochemistry and ELISA were used to investigate the protein expression levels of inflammasomes and ADAMTS4. The results demonstrated that all four inflammasomes were elevated in placenta and fetal membrane of PPROMs as were mRNA and protein expression levels of IL‑18 and IL‑1β (compared with controls). A further increase of inflammasomes and interleukins was observed in MPROMs compared with controls. Similar results were also observed in ADAMTS4 expression in PPROM and MPROM groups. However, immunohistochemistry results revealed no significant difference of inflammasome receptor expression in PPROMs compared with controls. Finally, a general positive correlation between ADAMTS4 and all four inflammasome receptors in placenta and fetal membrane of PPROMs and MPROMs was observed. The present study revealed that NLRP1, NLRP3, AIM2 and NLRC4 inflammasome activation in PROM was increased. Promoted ADAMTS4 level was further observed in PROM group and was significantly correlated with inflammasome expression. Inhibition of inflammasome activation may provide a therapeutic target for clinical PROM treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu J, Ma C, Luan X, Li J, Peng F and Huang L: Inflammasome components and ADAMTS4 in premature rupture of membranes. Mol Med Rep 23: 101, 2021.
APA
Zhu, J., Ma, C., Luan, X., Li, J., Peng, F., & Huang, L. (2021). Inflammasome components and ADAMTS4 in premature rupture of membranes. Molecular Medicine Reports, 23, 101. https://doi.org/10.3892/mmr.2020.11740
MLA
Zhu, J., Ma, C., Luan, X., Li, J., Peng, F., Huang, L."Inflammasome components and ADAMTS4 in premature rupture of membranes". Molecular Medicine Reports 23.2 (2021): 101.
Chicago
Zhu, J., Ma, C., Luan, X., Li, J., Peng, F., Huang, L."Inflammasome components and ADAMTS4 in premature rupture of membranes". Molecular Medicine Reports 23, no. 2 (2021): 101. https://doi.org/10.3892/mmr.2020.11740
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu J, Ma C, Luan X, Li J, Peng F and Huang L: Inflammasome components and ADAMTS4 in premature rupture of membranes. Mol Med Rep 23: 101, 2021.
APA
Zhu, J., Ma, C., Luan, X., Li, J., Peng, F., & Huang, L. (2021). Inflammasome components and ADAMTS4 in premature rupture of membranes. Molecular Medicine Reports, 23, 101. https://doi.org/10.3892/mmr.2020.11740
MLA
Zhu, J., Ma, C., Luan, X., Li, J., Peng, F., Huang, L."Inflammasome components and ADAMTS4 in premature rupture of membranes". Molecular Medicine Reports 23.2 (2021): 101.
Chicago
Zhu, J., Ma, C., Luan, X., Li, J., Peng, F., Huang, L."Inflammasome components and ADAMTS4 in premature rupture of membranes". Molecular Medicine Reports 23, no. 2 (2021): 101. https://doi.org/10.3892/mmr.2020.11740
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