Open Access

Effect of EZH2 on pulmonary artery smooth muscle cell migration in pulmonary hypertension

  • Authors:
    • Ying Wang
    • Xiao-Xi Huang
    • Dong Leng
    • Ji-Feng Li
    • Yan Liang
    • Tao Jiang
  • View Affiliations

  • Published online on: December 10, 2020     https://doi.org/10.3892/mmr.2020.11768
  • Article Number: 129
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Pulmonary hypertension (PH) is a life‑threatening disease that often involves vascular remodeling. Although pulmonary arterial smooth muscle cells (PASMCs) are the primary participants in vascular remodeling, their biological role is not entirely clear. The present study analyzed the role of enhancer of zeste homolog 2 (EZH2) in vascular remodeling of PH by investigating the behavior of PASMCs. The expression levels of EZH2 in PASMCs in chronic thromboembolic pulmonary hypertension (CTEPH), a type of PH, were detected. The role of EZH2 in PASMC migration was investigated by wound‑healing assay following overexpression and knockdown. Functional enrichment analysis of the whole‑genome expression profiles of PASMCs with EZH2 overexpression was performed using an mRNA Human Gene Expression Microarray. Quantitative (q)PCR was performed to confirm the results of the microarray. EZH2 expression levels increased in CTEPH cell models. The overexpression of EZH2 enhanced PASMC migration compared with control conditions. Functional enrichment analysis of the differentially expressed genes following EZH2 overexpression indicated a strong link between EZH2 and the immune inflammatory response and oxidoreductase activity in PASMCs. mRNA expression levels of superoxide dismutase 3 were verified by qPCR. The results suggested that EZH2 was involved in the migration of PASMCs in PH, and may serve as a potential target for the treatment of PH.
View Figures
View References

Related Articles

Journal Cover

February-2021
Volume 23 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Huang X, Leng D, Li J, Liang Y and Jiang T: Effect of EZH2 on pulmonary artery smooth muscle cell migration in pulmonary hypertension. Mol Med Rep 23: 129, 2021
APA
Wang, Y., Huang, X., Leng, D., Li, J., Liang, Y., & Jiang, T. (2021). Effect of EZH2 on pulmonary artery smooth muscle cell migration in pulmonary hypertension. Molecular Medicine Reports, 23, 129. https://doi.org/10.3892/mmr.2020.11768
MLA
Wang, Y., Huang, X., Leng, D., Li, J., Liang, Y., Jiang, T."Effect of EZH2 on pulmonary artery smooth muscle cell migration in pulmonary hypertension". Molecular Medicine Reports 23.2 (2021): 129.
Chicago
Wang, Y., Huang, X., Leng, D., Li, J., Liang, Y., Jiang, T."Effect of EZH2 on pulmonary artery smooth muscle cell migration in pulmonary hypertension". Molecular Medicine Reports 23, no. 2 (2021): 129. https://doi.org/10.3892/mmr.2020.11768