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Mitochondrial proteomics alterations in rat hearts following ischemia/reperfusion and diazoxide post‑conditioning

  • Authors:
    • Yunchao Pan
    • Yuan Wang
    • Wenyan Shi
    • Yun Liu
    • Song Cao
    • Tian Yu
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563000, P.R. China, Department of Anesthesiology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563003, P.R. China, Research Center for Medicine and Biology, Zunyi Medical University, Zunyi, Guizhou 563003, P.R. China, Department of Pain Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563000, P.R. China, Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi Medical University, Zunyi, Guizhou 563003, P.R. China
    Copyright: © Pan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 161
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    Published online on: December 22, 2020
       https://doi.org/10.3892/mmr.2020.11800
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Abstract

Diazoxide post‑conditioning (D‑Post) has been shown to be effective in alleviating myocardial ischemia/reperfusion (I/R) injury; however, the specific mechanisms are not fully understood. In the present study, isolated rat hearts were subjected to I/R injury and D‑Post. The mitochondria were extracted, and mitochondrial protein expression was detected in normal, I/R and D‑Post hearts using two‑dimensional electrophoresis and matrix‑assisted laser desorption ionization‑time of flight mass spectrometry. Differentially expressed proteins were then identified using comparative proteomics. In total, five differentially expressed proteins were identified between the I/R and D‑Post hearts. Compared with the I/R hearts, the expression of NADH dehydrogenase (ubiquinone) flavoprotein 1 (NDUFV1), NADH‑ubiquinone oxidoreductase 75 kDa subunit (NDUFS1), 2‑oxoglutarate dehydrogenase (OGDH) and ATP synthase α subunit (isoform CRA_b, gi|149029482) was increased in D‑Post hearts. In addition, the expression of another isoform of ATP synthase α subunit (isoform CRA_c, gi|149029480) was decreased in the D‑Post group compared with the I/R group. The expression profiles of NDUFV1, NDUFS1 and OGDH in the two groups were further validated via western blotting. The five differentially expressed proteins may be protective effectors in D‑Post, as well as potential targets for the treatment of cardiac I/R injury.
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Copy and paste a formatted citation
Spandidos Publications style
Pan Y, Wang Y, Shi W, Liu Y, Cao S and Yu T: Mitochondrial proteomics alterations in rat hearts following ischemia/reperfusion and diazoxide post‑conditioning. Mol Med Rep 23: 161, 2021.
APA
Pan, Y., Wang, Y., Shi, W., Liu, Y., Cao, S., & Yu, T. (2021). Mitochondrial proteomics alterations in rat hearts following ischemia/reperfusion and diazoxide post‑conditioning. Molecular Medicine Reports, 23, 161. https://doi.org/10.3892/mmr.2020.11800
MLA
Pan, Y., Wang, Y., Shi, W., Liu, Y., Cao, S., Yu, T."Mitochondrial proteomics alterations in rat hearts following ischemia/reperfusion and diazoxide post‑conditioning". Molecular Medicine Reports 23.2 (2021): 161.
Chicago
Pan, Y., Wang, Y., Shi, W., Liu, Y., Cao, S., Yu, T."Mitochondrial proteomics alterations in rat hearts following ischemia/reperfusion and diazoxide post‑conditioning". Molecular Medicine Reports 23, no. 2 (2021): 161. https://doi.org/10.3892/mmr.2020.11800
Copy and paste a formatted citation
x
Spandidos Publications style
Pan Y, Wang Y, Shi W, Liu Y, Cao S and Yu T: Mitochondrial proteomics alterations in rat hearts following ischemia/reperfusion and diazoxide post‑conditioning. Mol Med Rep 23: 161, 2021.
APA
Pan, Y., Wang, Y., Shi, W., Liu, Y., Cao, S., & Yu, T. (2021). Mitochondrial proteomics alterations in rat hearts following ischemia/reperfusion and diazoxide post‑conditioning. Molecular Medicine Reports, 23, 161. https://doi.org/10.3892/mmr.2020.11800
MLA
Pan, Y., Wang, Y., Shi, W., Liu, Y., Cao, S., Yu, T."Mitochondrial proteomics alterations in rat hearts following ischemia/reperfusion and diazoxide post‑conditioning". Molecular Medicine Reports 23.2 (2021): 161.
Chicago
Pan, Y., Wang, Y., Shi, W., Liu, Y., Cao, S., Yu, T."Mitochondrial proteomics alterations in rat hearts following ischemia/reperfusion and diazoxide post‑conditioning". Molecular Medicine Reports 23, no. 2 (2021): 161. https://doi.org/10.3892/mmr.2020.11800
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