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Nogo‑66 promotes β‑amyloid protein secretion via NgR/ROCK‑dependent BACE1 activation

  • Authors:
    • Qing-Qing Xie
    • Xiao Feng
    • Yi-Υun Huang
    • Nian Fang
    • Hua Yi
    • Zi-Jian Wang
    • Qiao-Υu Cao
    • Guo-Feng Lou
    • Jun-Ping Pan
    • Yang Hu
    • Fang-Cheng Li
    • Qing Zheng
    • Fei Xiao
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, School of Medicine, Jinan University, Guangzhou, Guangdong 510632, P.R. China, Department of Microbiology and Biochemical Pharmacy, School of Pharmacy, Jinan University, Guangzhou, Guangdong 510632, P.R. China, Department of Pathology, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 511436, P.R. China, Department of Clinical Molecular Biology, Akershus University Hospital, University of Oslo, 1478 Lørenskog, Norway, Department of Neurosurgery, Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong 510632, P.R. China
    Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 188
    |
    Published online on: January 5, 2021
       https://doi.org/10.3892/mmr.2021.11827
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Abstract

The generation of β‑amyloid protein (Aβ) is considered a key step in the pathogenesis of Alzheimer's disease (AD) and the regulation of its production is an important therapeutic strategy. It was hypothesized in the present study that Nogo‑A may be involved in AD and may regulate the generation of Aβ. Nogo‑A is known to act as a major inhibitor of neuron regeneration in the adult central nervous system. A recent study indicated that Nogo‑A is associated with AD; however, the underlying effect and molecular mechanisms remain largely elusive. In the present study, the potential effects of Nogo‑A on AD were investigated. ELISA was used to detect the levels of Aβ, enzymatic activity detection kits were used to determine the activity of secretase enzymes in amyloid precursor protein (APP) metabolism, and western blot analysis was used to detect the expression levels of proteins associated with the APP processing and Nogo‑A/Nogo‑66 receptor (NgR) signaling pathways. The results revealed that Nogo‑66, the major inhibitory region of Nogo‑A, promoted neuronal Aβ secretion by increasing the activity of β‑secretase 1 via the NgR/Rho‑associated coiled‑coil containing kinases pathway in a dose‑dependent manner. The present data suggested that Nogo‑A may facilitate the onset and development of AD by promoting Aβ secretion, providing information on a potential novel target for AD therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Xie Q, Feng X, Huang Y, Fang N, Yi H, Wang Z, Cao Q, Lou G, Pan J, Hu Y, Hu Y, et al: Nogo‑66 promotes β‑amyloid protein secretion via NgR/ROCK‑dependent BACE1 activation. Mol Med Rep 23: 188, 2021.
APA
Xie, Q., Feng, X., Huang, Y., Fang, N., Yi, H., Wang, Z. ... Xiao, F. (2021). Nogo‑66 promotes β‑amyloid protein secretion via NgR/ROCK‑dependent BACE1 activation. Molecular Medicine Reports, 23, 188. https://doi.org/10.3892/mmr.2021.11827
MLA
Xie, Q., Feng, X., Huang, Y., Fang, N., Yi, H., Wang, Z., Cao, Q., Lou, G., Pan, J., Hu, Y., Li, F., Zheng, Q., Xiao, F."Nogo‑66 promotes β‑amyloid protein secretion via NgR/ROCK‑dependent BACE1 activation". Molecular Medicine Reports 23.3 (2021): 188.
Chicago
Xie, Q., Feng, X., Huang, Y., Fang, N., Yi, H., Wang, Z., Cao, Q., Lou, G., Pan, J., Hu, Y., Li, F., Zheng, Q., Xiao, F."Nogo‑66 promotes β‑amyloid protein secretion via NgR/ROCK‑dependent BACE1 activation". Molecular Medicine Reports 23, no. 3 (2021): 188. https://doi.org/10.3892/mmr.2021.11827
Copy and paste a formatted citation
x
Spandidos Publications style
Xie Q, Feng X, Huang Y, Fang N, Yi H, Wang Z, Cao Q, Lou G, Pan J, Hu Y, Hu Y, et al: Nogo‑66 promotes β‑amyloid protein secretion via NgR/ROCK‑dependent BACE1 activation. Mol Med Rep 23: 188, 2021.
APA
Xie, Q., Feng, X., Huang, Y., Fang, N., Yi, H., Wang, Z. ... Xiao, F. (2021). Nogo‑66 promotes β‑amyloid protein secretion via NgR/ROCK‑dependent BACE1 activation. Molecular Medicine Reports, 23, 188. https://doi.org/10.3892/mmr.2021.11827
MLA
Xie, Q., Feng, X., Huang, Y., Fang, N., Yi, H., Wang, Z., Cao, Q., Lou, G., Pan, J., Hu, Y., Li, F., Zheng, Q., Xiao, F."Nogo‑66 promotes β‑amyloid protein secretion via NgR/ROCK‑dependent BACE1 activation". Molecular Medicine Reports 23.3 (2021): 188.
Chicago
Xie, Q., Feng, X., Huang, Y., Fang, N., Yi, H., Wang, Z., Cao, Q., Lou, G., Pan, J., Hu, Y., Li, F., Zheng, Q., Xiao, F."Nogo‑66 promotes β‑amyloid protein secretion via NgR/ROCK‑dependent BACE1 activation". Molecular Medicine Reports 23, no. 3 (2021): 188. https://doi.org/10.3892/mmr.2021.11827
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