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Genistein alleviates chronic vascular inflammatory response via the miR‑21/NF‑κB p65 axis in lipopolysaccharide‑treated mice

  • Authors:
    • Xiaolin Xie
    • Li Cong
    • Sujuan Liu
    • Liping Xiang
    • Xiaohua Fu
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, Hunan Normal University, Changsha, Hunan 410013, P.R. China, Department of Basic Medicine, School of Medicine, Hunan Normal University, Changsha, Hunan 410013, P.R. China, Department of Pathology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
    Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 192
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    Published online on: January 7, 2021
       https://doi.org/10.3892/mmr.2021.11831
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Abstract

Chronic vascular inflammatory response is an important pathological basis of cardiovascular disease. Genistein (GEN), a natural compound, exhibits anti‑inflammatory effects. The aim of the present study was to investigate the effects of GEN on lipopolysaccharide (LPS)‑induced chronic vascular inflammatory response in mice and explore the underlying anti‑inflammatory mechanisms. C57BL/6 mice were fed with a high‑fat diet combined with intraperitoneal injection of LPS to induce chronic vascular inflammation. The expression levels of TNF‑α, IL‑6 and microRNA (miR)‑21 in the vasculature were detected via reverse transcription‑quantitative (RT‑q)PCR. The protein levels of inducible nitric oxide synthase (iNOS) and NF‑κB p65 were detected via western blotting. NF‑κB p65 was also analyzed via immunohistochemistry and immunofluorescence (IF). In addition, after transfection with miR‑21 mimic or inhibitor for 24 h, vascular endothelial cells (VECs) were treated with GEN and LPS. RT‑qPCR and western blot analyses were performed to detect the expression of TNF‑α, IL‑6, miR‑21 and iNOS, and the protein levels of iNOS and NF‑κB p65, respectively. IF was used to measure NF‑κB p65 nuclear translocation. The results revealed that GEN significantly decreased the expression of inflammation‑associated vascular factors in LPS‑treated C57BL/6 mice, including TNF‑α, IL‑6, iNOS, NF‑κB p65 and miR‑21. Furthermore, miR‑21 antagomir enhanced the anti‑inflammatory effects of GEN. In LPS‑induced VECs, miR‑21 mimic increased inflammation‑associated factor expression and attenuated the anti‑inflammatory effects of GEN, whereas miR‑21 inhibitor induced opposing effects. Therefore, the results of the present study suggested that GEN inhibited chronic vascular inflammatory response in mice, which may be associated with the inhibition of VEC inflammatory injury via the miR‑21/NF‑κB p65 pathway.
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Xie X, Cong L, Liu S, Xiang L and Fu X: Genistein alleviates chronic vascular inflammatory response via the miR‑21/NF‑κB p65 axis in lipopolysaccharide‑treated mice. Mol Med Rep 23: 192, 2021.
APA
Xie, X., Cong, L., Liu, S., Xiang, L., & Fu, X. (2021). Genistein alleviates chronic vascular inflammatory response via the miR‑21/NF‑κB p65 axis in lipopolysaccharide‑treated mice. Molecular Medicine Reports, 23, 192. https://doi.org/10.3892/mmr.2021.11831
MLA
Xie, X., Cong, L., Liu, S., Xiang, L., Fu, X."Genistein alleviates chronic vascular inflammatory response via the miR‑21/NF‑κB p65 axis in lipopolysaccharide‑treated mice". Molecular Medicine Reports 23.3 (2021): 192.
Chicago
Xie, X., Cong, L., Liu, S., Xiang, L., Fu, X."Genistein alleviates chronic vascular inflammatory response via the miR‑21/NF‑κB p65 axis in lipopolysaccharide‑treated mice". Molecular Medicine Reports 23, no. 3 (2021): 192. https://doi.org/10.3892/mmr.2021.11831
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Spandidos Publications style
Xie X, Cong L, Liu S, Xiang L and Fu X: Genistein alleviates chronic vascular inflammatory response via the miR‑21/NF‑κB p65 axis in lipopolysaccharide‑treated mice. Mol Med Rep 23: 192, 2021.
APA
Xie, X., Cong, L., Liu, S., Xiang, L., & Fu, X. (2021). Genistein alleviates chronic vascular inflammatory response via the miR‑21/NF‑κB p65 axis in lipopolysaccharide‑treated mice. Molecular Medicine Reports, 23, 192. https://doi.org/10.3892/mmr.2021.11831
MLA
Xie, X., Cong, L., Liu, S., Xiang, L., Fu, X."Genistein alleviates chronic vascular inflammatory response via the miR‑21/NF‑κB p65 axis in lipopolysaccharide‑treated mice". Molecular Medicine Reports 23.3 (2021): 192.
Chicago
Xie, X., Cong, L., Liu, S., Xiang, L., Fu, X."Genistein alleviates chronic vascular inflammatory response via the miR‑21/NF‑κB p65 axis in lipopolysaccharide‑treated mice". Molecular Medicine Reports 23, no. 3 (2021): 192. https://doi.org/10.3892/mmr.2021.11831
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