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Article

Effect of Notch1 on neural tube defects and neural stem cell differentiation induced by all‑trans retinoic acid

  • Authors:
    • Na Chen
    • Jiaming Xu
    • Xinying Zhang
    • Shuwei Li
    • Weiwei Zhu
    • Haibin Cui
    • Yu Sun
    • Bo Han
    • Aihua Ma
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, P.R. China, Department of Pediatrics, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China
  • Article Number: 220
    |
    Published online on: January 21, 2021
       https://doi.org/10.3892/mmr.2021.11859
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Abstract

Neural tube defects (NTDs) are the most serious and common birth defects in the clinical setting. The Notch signaling pathway has been implicated in different processes of the embryonic neural stem cells (NSCs) during neural tube development. The aim of the present study was to investigate the expression pattern and function of Notch1 (N1) in all‑trans retinoic acid (atRA)‑induced NTDs and NSC differentiation. A mouse model of brain abnormality was established by administering 28 mg/kg atRA, and then brain development was examined using hematoxylin and eosin (H&E) staining. The N1 expression pattern was detected in the brain of mice embryos via immunohistochemistry and western blotting. NSCs were extracted from the fetal brain of C57 BL/6 embryos at 18.5 days of pregnancy. N1, Nestin, neurofilament (NF), glial fibrillary acidic protein (GFAP) and galactocerebroside (GALC) were identified using immunohistochemistry. Moreover, N1, presenilin 1 (PS1), Nestin, NF, GFAP and GALC were detected via western blotting at different time points in the NSCs with control media or atRA media. H&E staining identified that the embryonic brain treated with atRA was more developed compared with the control group. N1 was downregulated in the embryonic mouse brain between days 11 and 17 in the atRA‑treated group compared with the untreated group. The distribution of N1, Nestin, NF, GFAP and GALC was positively detected using immunofluorescence staining. Western blotting results demonstrated that there were significantly, synchronous decreased expression levels of N1 and PS1, but increased expression levels of NF, GFAP and GALC in NSCs treated with atRA compared with those observed in the controls (P<0.05). The results suggested that the N1 signaling pathway inhibited brain development and NSC differentiation. Collectively, it was found that atRA promoted mouse embryo brain development and the differentiation of NSCs by inhibiting the N1 pathway.
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1 

Zaganjor I, Sekkarie A, Tsang BL, Williams J, Razzaghi H, Mulinare J, Sniezek JE, Cannon MJ and Rosenthal J: Describing the Prevalence of Neural Tube Defects Worldwide: A Systematic Literature Review. PLoS One. 11:e01515862016. View Article : Google Scholar : PubMed/NCBI

2 

Copp AJ and Greene ND: Neural tube defects - disorders of neurulation and related embryonic processes. Wiley Interdiscip Rev Dev Biol. 2:213–227. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Copp AJ, Stanier P and Greene ND: Neural tube defects: Recent advances, unsolved questions, and controversies. Lancet Neurol. 12:799–810. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Lowery LA and Sive H: Strategies of vertebrate neurulation and a re-evaluation of teleost neural tube formation. Mech Dev. 121:1189–1197. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Nikolopoulou E, Galea GL, Rolo A, Greene ND and Copp AJ: Neural tube closure: cellular, molecular and biomechanical mechanisms. Development. 144:552–566. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Liang S, Yin N and Faiola F: Human Pluripotent Stem Cells as Tools for Predicting Developmental Neural Toxicity of Chemicals: Strategies, Applications, and Challenges. Stem Cells Dev. 28:755–768. 2019. View Article : Google Scholar : PubMed/NCBI

7 

Daadi MM: Generation of Neural Stem Cells from Induced Pluripotent Stem Cells. Methods Mol Biol. 1919:1–7. 2019. View Article : Google Scholar : PubMed/NCBI

8 

Artavanis-Tsakonas S, Rand MD and Lake RJ: Notch signaling: Cell fate control and signal integration in development. Science. 284:770–776. 1999. View Article : Google Scholar : PubMed/NCBI

9 

Andersson ER, Sandberg R and Lendahl U: Notch signaling: Simplicity in design, versatility in function. Development. 138:3593–3612. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Bray SJ: Notch signalling in context. Nat Rev Mol Cell Biol. 17:722–735. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Ni X, Hu G and Cai X: The success and the challenge of all-trans retinoci acid in the treatment of cancer. Crit Rev Food Sci Nutr. 59 (Suppl 1):S71–S80. 2019. View Article : Google Scholar : PubMed/NCBI

12 

Sewell W and Kusumi K: Genetic analysis of molecular oscillators in mammalian somitogenesis: Clues for studies of human vertebral disorders. Birth Defects Res C Embryo Today. 81:111–120. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Liu D, Xue J, Liu Y, Gu H, Wei X, Ma W, Luo W, Ma L, Jia S, Dong N, et al: Inhibition of NRF2 signaling and increased reactive oxygen species during embryogenesis in a rat model of retinoic acid-induced neural tube defects. Neurotoxicology. 69:84–92. 2018. View Article : Google Scholar : PubMed/NCBI

14 

Seegmiller RE, Ford WH, Carter MW, Mitala JJ and Powers WJ Jr: A developmental toxicity study of tretinoin administered topically and orally to pregnant Wistar rats. J Am Acad Dermatol. 36:S60–S66. 1997. View Article : Google Scholar : PubMed/NCBI

15 

El Hajj A, Yen FT, Oster T, Malaplate C, Pauron L, Corbier C, Lanhers MC and Claudepierre T: Age-related changes in regiospecific expression of Lipolysis Stimulated Receptor (LSR) in mice brain. PLoS One. 14:e02188122019. View Article : Google Scholar : PubMed/NCBI

16 

Cearns MD, Escuin S, Alexandre P, Greene ND and Copp AJ: Microtubules, polarity and vertebrate neural tube morphogenesis. J Anat. 229:63–74. 2016. View Article : Google Scholar : PubMed/NCBI

17 

McShane SG, Molè MA, Savery D, Greene ND, Tam PP and Copp AJ: Cellular basis of neuroepithelial bending during mouse spinal neural tube closure. Dev Biol. 404:113–124. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Leung KY, Pai YJ, Chen Q, Santos C, Calvani E, Sudiwala S, Savery D, Ralser M, Gross SS, Copp AJ, et al: Partitioning of one-carbon units in folate and methionine metabolism is essential for neural tube closure. Cell Rep. 21:1795–1808. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Nau H, Hauck RS and Ehlers K: Valproic acid-induced neural tube defects in mouse and human: Aspects of chirality, alternative drug development, pharmacokinetics and possible mechanisms. Pharmacol Toxicol. 69:310–321. 1991. View Article : Google Scholar : PubMed/NCBI

20 

Jin L, Zhang L, Li Z, Liu JM, Ye R and Ren A: Placental concentrations of mercury, lead, cadmium, and arsenic and the risk of neural tube defects in a Chinese population. Reprod Toxicol. 35:25–31. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Ren A, Qiu X, Jin L, Ma J, Li Z, Zhang L, Zhu H, Finnell RH and Zhu T: Association of selected persistent organic pollutants in the placenta with the risk of neural tube defects. Proc Natl Acad Sci USA. 108:12770–12775. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Kanakasabai S, Pestereva E, Chearwae W, Gupta SK, Ansari S and Bright JJ: PPARγ agonists promote oligodendrocyte differentiation of neural stem cells by modulating stemness and differentiation genes. PLoS One. 7:e505002012. View Article : Google Scholar : PubMed/NCBI

23 

Mohlin S, Kunttas E, Persson CU, Abdel-Haq R, Castillo A, Murko C, Bronner ME and Kerosuo L: Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres. Dev Biol. 447:137–146. 2019. View Article : Google Scholar : PubMed/NCBI

24 

Urata Y, Yamashita W, Inoue T and Agata K: Spatio-temporal neural stem cell behavior leads to both perfect and imperfect structural brain regeneration in adult newts. Biol Open. 7:bio0331422018. View Article : Google Scholar : PubMed/NCBI

25 

Zhang J, Li R, He Q, Li WI, Niu B, Cheng N, Zhou R, Zhang T, Zheng X and Xie J: All-trans-retinoic acid alters Smads expression in embryonic neural tissue of mice. J Appl Toxicol. 29:364–366. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Lee SH, Shin JH, Shin MH, Kim YS, Chung KS, Song JH, Kim SY, Kim EY, Jung JY, Kang YA, et al: The effects of retinoic acid and MAPK inhibitors on phosphorylation of Smad2/3 induced by transforming growth factor β1. Tuberc Respir Dis (Seoul). 82:42–52. 2019. View Article : Google Scholar : PubMed/NCBI

27 

Ryu HW, Park CW and Ryu KY: Disruption of polyubiquitin gene Ubb causes dysregulation of neural stem cell differentiation with premature gliogenesis. Sci Rep. 4:70262014. View Article : Google Scholar : PubMed/NCBI

28 

Ying M, Wang S, Sang Y, Sun P, Lal B, Goodwin CR, Guerrero-Cazares H, Quinones-Hinojosa A, Laterra J and Xia S: Regulation of glioblastoma stem cells by retinoic acid: Role for Notch pathway inhibition. Oncogene. 30:3454–3467. 2011. View Article : Google Scholar : PubMed/NCBI

29 

De Filippis L, Zalfa C and Ferrari D: Neural Stem Cells and Human Induced Pluripotent Stem Cells to Model Rare CNS Diseases. CNS Neurol Disord Drug Targets. 16:915–926. 2017.PubMed/NCBI

30 

Dunnett SB and Rosser AE: Cell transplantation for Huntington's disease Should we continue? Brain Res Bull. 72:132–147. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Li J, Pauley AM, Myers RL, Shuang R, Brashler JR, Yan R, Buhl AE, Ruble C and Gurney ME: SEL-10 interacts with presenilin 1, facilitates its ubiquitination, and alters A-beta peptide production. J Neurochem. 82:1540–1548. 2002. View Article : Google Scholar : PubMed/NCBI

32 

Duggan SP and McCarthy JV: Beyond γ-secretase activity: The multifunctional nature of presenilins in cell signalling pathways. Cell Signal. 28:1–11. 2016. View Article : Google Scholar : PubMed/NCBI

33 

De Strooper B, Annaert W, Cupers P, Saftig P, Craessaerts K, Mumm JS, Schroeter EH, Schrijvers V, Wolfe MS, Ray WJ, et al: A presenilin-1-dependent gamma-secretase-like protease mediates release of Notch intracellular domain. Nature. 398:518–522. 1999. View Article : Google Scholar : PubMed/NCBI

34 

Peng Z, Li X, Fu M, Zhu K, Long L, Zhao X, Chen Q, Deng DYB and Wan Y: Inhibition of Notch1 signaling promotes neuronal differentiation and improves functional recovery in spinal cord injury through suppressing the activation of Ras homolog family member A. J Neurochem. 150:709–722. 2019. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen N, Xu J, Zhang X, Li S, Zhu W, Cui H, Sun Y, Han B and Ma A: Effect of Notch1 on neural tube defects and neural stem cell differentiation induced by all‑<em>trans</em> retinoic acid. Mol Med Rep 23: 220, 2021.
APA
Chen, N., Xu, J., Zhang, X., Li, S., Zhu, W., Cui, H. ... Ma, A. (2021). Effect of Notch1 on neural tube defects and neural stem cell differentiation induced by all‑<em>trans</em> retinoic acid. Molecular Medicine Reports, 23, 220. https://doi.org/10.3892/mmr.2021.11859
MLA
Chen, N., Xu, J., Zhang, X., Li, S., Zhu, W., Cui, H., Sun, Y., Han, B., Ma, A."Effect of Notch1 on neural tube defects and neural stem cell differentiation induced by all‑<em>trans</em> retinoic acid". Molecular Medicine Reports 23.3 (2021): 220.
Chicago
Chen, N., Xu, J., Zhang, X., Li, S., Zhu, W., Cui, H., Sun, Y., Han, B., Ma, A."Effect of Notch1 on neural tube defects and neural stem cell differentiation induced by all‑<em>trans</em> retinoic acid". Molecular Medicine Reports 23, no. 3 (2021): 220. https://doi.org/10.3892/mmr.2021.11859
Copy and paste a formatted citation
x
Spandidos Publications style
Chen N, Xu J, Zhang X, Li S, Zhu W, Cui H, Sun Y, Han B and Ma A: Effect of Notch1 on neural tube defects and neural stem cell differentiation induced by all‑<em>trans</em> retinoic acid. Mol Med Rep 23: 220, 2021.
APA
Chen, N., Xu, J., Zhang, X., Li, S., Zhu, W., Cui, H. ... Ma, A. (2021). Effect of Notch1 on neural tube defects and neural stem cell differentiation induced by all‑<em>trans</em> retinoic acid. Molecular Medicine Reports, 23, 220. https://doi.org/10.3892/mmr.2021.11859
MLA
Chen, N., Xu, J., Zhang, X., Li, S., Zhu, W., Cui, H., Sun, Y., Han, B., Ma, A."Effect of Notch1 on neural tube defects and neural stem cell differentiation induced by all‑<em>trans</em> retinoic acid". Molecular Medicine Reports 23.3 (2021): 220.
Chicago
Chen, N., Xu, J., Zhang, X., Li, S., Zhu, W., Cui, H., Sun, Y., Han, B., Ma, A."Effect of Notch1 on neural tube defects and neural stem cell differentiation induced by all‑<em>trans</em> retinoic acid". Molecular Medicine Reports 23, no. 3 (2021): 220. https://doi.org/10.3892/mmr.2021.11859
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