Open Access

Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL‑1β pathway

  • Authors:
    • Xianzhen Zhang
    • Huideng Ding
    • Gang Hu
    • Ying Lu
  • View Affiliations

  • Published online on: January 24, 2021     https://doi.org/10.3892/mmr.2021.11862
  • Article Number: 223
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies have suggested that the herbal medicine simiaosan has beneficial effects on gouty arthritis (GA), for which conventional Western medicines are insufficient (particularly in cases of multiple episodes). The objective of the present study was to investigate the mechanism by which simiaosan alleviated the symptoms of GA. Sprague‑Dawley rat models of acute GA were successfully established, as verified by pathological analyses. Additionally, an NLR family pyrin domain containing 3 (NLRP3) overexpression vector was constructed and a high transfection efficiency was confirmed by reverse transcription PCR. The following five treatment groups were established: i) Normal control; ii) model + saline; iii) model + simiaosan; iv) model + NALP3‑overexpressing adenovirus + simiaosan; and v) model + empty vector adenovirus + simiaosan. The samples from mice in each group were subjected to hematoxylin and eosin (H&E) staining for assessing the histopathological changes, enzyme‑linked immunosorbent assays for determining IL‑1β and TGF‑β1 levels and western blotting for evaluating NALP3 expression. H&E staining indicated that simiaosan could reduce the infiltration of inflammatory cells, while NALP3 overexpression aggravated the inflammatory response in tissues. Expression levels of IL‑1β, TGF‑β1 and NALP3 were significantly higher in the model and the model + NALP3‑overexpressing adenovirus + simiaosan groups compared with the normal control group. Levels of IL‑1β, TGF‑β1 and NALP3 were significantly lower in the model + simiaosan and model + empty vector adenovirus + simiaosan groups compared with the model group. These results indicated that the effects of simiaosan were mediated through NALP3 inhibition. Therefore, the herbal medicine simiaosan was revealed to possess an ability to alleviate the symptoms of GA by regulating the NALP3/IL‑1β signaling pathway.
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March-2021
Volume 23 Issue 3

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Copy and paste a formatted citation
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Spandidos Publications style
Zhang X, Ding H, Hu G and Lu Y: Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL‑1β pathway. Mol Med Rep 23: 223, 2021
APA
Zhang, X., Ding, H., Hu, G., & Lu, Y. (2021). Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL‑1β pathway. Molecular Medicine Reports, 23, 223. https://doi.org/10.3892/mmr.2021.11862
MLA
Zhang, X., Ding, H., Hu, G., Lu, Y."Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL‑1β pathway". Molecular Medicine Reports 23.3 (2021): 223.
Chicago
Zhang, X., Ding, H., Hu, G., Lu, Y."Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL‑1β pathway". Molecular Medicine Reports 23, no. 3 (2021): 223. https://doi.org/10.3892/mmr.2021.11862