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Metabolomic profiling identifies a novel mechanism for heat stroke‑related acute kidney injury

  • Authors:
    • Ling Xue
    • Wenli Guo
    • Li Li
    • Santao Ou
    • Tingting Zhu
    • Liang Cai
    • Wenfei Ding
    • Weihua Wu
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Sichuan Clinical Research Center for Nephropathy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, Department of Nephrology, Sichuan Clinical Research Center for Nephropathy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, Department of Nuclear Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China
    Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 241
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    Published online on: January 28, 2021
       https://doi.org/10.3892/mmr.2021.11880
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Abstract

Heat stroke can induce a systemic inflammatory response, which may lead to multi‑organ dysfunction including acute kidney injury (AKI) and electrolyte disturbances. To investigate the pathogenesis of heat stroke (HS)‑related AKI, a mouse model of HS was induced by increasing the animal's core temperature to 41˚C. Blood samples obtained from the tail vein were used to measure plasma glucose and creatinine levels. Micro‑positron emission tomography‑computed tomography (micro‑PET/CT), H&E staining and transmission electron microscopy were conducted to examine metabolic and morphological changes in the mouse kidneys. Immunohistochemistry (IHC) and western blot analyses were performed to investigate the expression of apoptosis‑inducing factor mitochondria‑associated 2 (Aifm2), high‑mobility group box 1 (HMGB1) and receptor for advanced glycosylation end products (RAGE). Liquid chromatography‑mass spectrometry analysis was conducted to find differential metabolites and signaling pathways. The HS mouse model was built successfully, with significantly increased creatinine levels detected in the serum of HS mice compared with controls, whereas micro‑PET/CT revealed active metabolism in the whole body of HS mice. H&E and TUNEL staining revealed that the kidneys of HS mice exhibited signs of hemorrhage and apoptosis. IHC and western blotting demonstrated significant upregulation of Aifm2, HMGB1 and RAGE in response to HS. Finally, 136 differential metabolites were screened out, and enrichment of the ‘biosynthesis of unsaturated fatty acids’ pathway was detected. HS‑associated AKI is the renal manifestation of systemic inflammatory response syndrome, and may be triggered by the HMGB1/RAGE pathway. Metabolomics indicated increased adrenic acid, docosahexaenoic acid and eicosapentaenoic acid may serve as metabolic biomarkers for AKI in HS. The findings suggested that a correlation between the HMGB1/RAGE pathway and biosynthesis of unsaturated fatty acids may contribute to the progression of HS‑related AKI.
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Xue L, Guo W, Li L, Ou S, Zhu T, Cai L, Ding W and Wu W: Metabolomic profiling identifies a novel mechanism for heat stroke‑related acute kidney injury. Mol Med Rep 23: 241, 2021.
APA
Xue, L., Guo, W., Li, L., Ou, S., Zhu, T., Cai, L. ... Wu, W. (2021). Metabolomic profiling identifies a novel mechanism for heat stroke‑related acute kidney injury. Molecular Medicine Reports, 23, 241. https://doi.org/10.3892/mmr.2021.11880
MLA
Xue, L., Guo, W., Li, L., Ou, S., Zhu, T., Cai, L., Ding, W., Wu, W."Metabolomic profiling identifies a novel mechanism for heat stroke‑related acute kidney injury". Molecular Medicine Reports 23.4 (2021): 241.
Chicago
Xue, L., Guo, W., Li, L., Ou, S., Zhu, T., Cai, L., Ding, W., Wu, W."Metabolomic profiling identifies a novel mechanism for heat stroke‑related acute kidney injury". Molecular Medicine Reports 23, no. 4 (2021): 241. https://doi.org/10.3892/mmr.2021.11880
Copy and paste a formatted citation
x
Spandidos Publications style
Xue L, Guo W, Li L, Ou S, Zhu T, Cai L, Ding W and Wu W: Metabolomic profiling identifies a novel mechanism for heat stroke‑related acute kidney injury. Mol Med Rep 23: 241, 2021.
APA
Xue, L., Guo, W., Li, L., Ou, S., Zhu, T., Cai, L. ... Wu, W. (2021). Metabolomic profiling identifies a novel mechanism for heat stroke‑related acute kidney injury. Molecular Medicine Reports, 23, 241. https://doi.org/10.3892/mmr.2021.11880
MLA
Xue, L., Guo, W., Li, L., Ou, S., Zhu, T., Cai, L., Ding, W., Wu, W."Metabolomic profiling identifies a novel mechanism for heat stroke‑related acute kidney injury". Molecular Medicine Reports 23.4 (2021): 241.
Chicago
Xue, L., Guo, W., Li, L., Ou, S., Zhu, T., Cai, L., Ding, W., Wu, W."Metabolomic profiling identifies a novel mechanism for heat stroke‑related acute kidney injury". Molecular Medicine Reports 23, no. 4 (2021): 241. https://doi.org/10.3892/mmr.2021.11880
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