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Effects of Cirsium setidens (Dunn) Nakai on the osteogenic differentiation of stem cells

  • Authors:
    • Sayan Deb Dutta
    • Dinesh K. Patel
    • Bin Jin
    • Sun-Il Choi
    • Ok Hwan Lee
    • Ki-Taek Lim
  • View Affiliations / Copyright

    Affiliations: Department of Biosystems Engineering, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Stomatology, Affiliated Hospital of Yanbian University, Yanji, Jilin 133000, P.R. China, Department of Food Science and Biotechnology, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea
    Copyright: © Dutta et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 264
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    Published online on: February 8, 2021
       https://doi.org/10.3892/mmr.2021.11903
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Abstract

Cirsium setidens (Dunn) Nakai, commonly known as gondre, is a perennial herb that grows predominantly in South Korea. It contains several bioactive phytochemicals with antioxidant, anti‑cancer, anti‑tumor and anti‑inflammatory properties. The present study aimed to investigate the effects of methanolic extracts of gondre on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs). As characterized by nuclear magnetic resonance spectroscopy and matrix‑assisted laser deposition/ionization (time‑of‑flight) mass spectrometry, the methanol extract of gondre was found to be enriched with pectolinarin. After 48 h, enhanced viability of hPDLSCs was observed in the presence of gondre compared with under control conditions, suggesting the biocompatibility of gondre. Notably, biocompatibility was markedly affected by gondre concentration in cultured media. Relatively high cell viability was observed in medium containing 0.05% gondre. Furthermore, mineralization was significantly higher in hPDLSCs in the presence of gondre compared with that in control cells, indicating their mineralization potential. Increased expression of various transcription markers, such as collagen 1, runt‑related transcription factor 2, bone sialoprotein and alkaline phosphatase, was also detected when hPDLSCs were stimulated with gondre compared with in the control groups, further confirming the superior osteogenic potential of gondre extract for tissue engineering applications, particularly in bone tissues.
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Copy and paste a formatted citation
Spandidos Publications style
Dutta SD, Patel DK, Jin B, Choi S, Lee OH and Lim K: Effects of <em>Cirsium setidens</em> (Dunn) Nakai on the osteogenic differentiation of stem cells. Mol Med Rep 23: 264, 2021.
APA
Dutta, S.D., Patel, D.K., Jin, B., Choi, S., Lee, O.H., & Lim, K. (2021). Effects of <em>Cirsium setidens</em> (Dunn) Nakai on the osteogenic differentiation of stem cells. Molecular Medicine Reports, 23, 264. https://doi.org/10.3892/mmr.2021.11903
MLA
Dutta, S. D., Patel, D. K., Jin, B., Choi, S., Lee, O. H., Lim, K."Effects of <em>Cirsium setidens</em> (Dunn) Nakai on the osteogenic differentiation of stem cells". Molecular Medicine Reports 23.4 (2021): 264.
Chicago
Dutta, S. D., Patel, D. K., Jin, B., Choi, S., Lee, O. H., Lim, K."Effects of <em>Cirsium setidens</em> (Dunn) Nakai on the osteogenic differentiation of stem cells". Molecular Medicine Reports 23, no. 4 (2021): 264. https://doi.org/10.3892/mmr.2021.11903
Copy and paste a formatted citation
x
Spandidos Publications style
Dutta SD, Patel DK, Jin B, Choi S, Lee OH and Lim K: Effects of <em>Cirsium setidens</em> (Dunn) Nakai on the osteogenic differentiation of stem cells. Mol Med Rep 23: 264, 2021.
APA
Dutta, S.D., Patel, D.K., Jin, B., Choi, S., Lee, O.H., & Lim, K. (2021). Effects of <em>Cirsium setidens</em> (Dunn) Nakai on the osteogenic differentiation of stem cells. Molecular Medicine Reports, 23, 264. https://doi.org/10.3892/mmr.2021.11903
MLA
Dutta, S. D., Patel, D. K., Jin, B., Choi, S., Lee, O. H., Lim, K."Effects of <em>Cirsium setidens</em> (Dunn) Nakai on the osteogenic differentiation of stem cells". Molecular Medicine Reports 23.4 (2021): 264.
Chicago
Dutta, S. D., Patel, D. K., Jin, B., Choi, S., Lee, O. H., Lim, K."Effects of <em>Cirsium setidens</em> (Dunn) Nakai on the osteogenic differentiation of stem cells". Molecular Medicine Reports 23, no. 4 (2021): 264. https://doi.org/10.3892/mmr.2021.11903
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