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Article

Anti‑fibrotic effects and the mechanism of action of miR‑29c in silicosis

  • Authors:
    • Lei Huang
    • Yang Zhang
    • Jie Yang
    • Juan Li
    • Ju Wu
    • Faxuan Wang
    • Yajia Lan
    • Qin Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Environmental Health and Occupational Medicine, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Periodical Press and National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Occupational Medicine, The Second People's Hospital of Panzhihua, Panzhihua, Sichuan 611300, P.R. China, Department of Respiratory and Critical Care Medicine, The Second People's Hospital of Panzhihua, Panzhihua, Sichuan 611300, P.R. China, Department of Occupational and Environmental Medicine, School of Public Health, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
  • Article Number: 292
    |
    Published online on: February 22, 2021
       https://doi.org/10.3892/mmr.2021.11932
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Abstract

Despite increasing evidence suggesting a role for the miR‑29 family in the suppression of fibrosis, its role in silicosis remains unknown. The present study aimed to examine the anti‑fibrotic effects and specific mechanism of action of microRNA (miR)‑29c in pulmonary silicosis using animal and cell models. miR‑29c expression levels were examined in the lungs of silicotic rats via reveres transcription‑quantitative (RT‑q)PCR. A Transwell system employing co‑cultures of pulmonary fibroblasts and macrophages was used to establish an in vitro cell model of silicosis, and lentivirus was used to overexpress or knockdown miR‑29c in cultured cells. Changes in collagen type I α I (COL1α1), COL3α1, α‑smooth muscle actin (α‑SMA) and TGF‑β1 expression levels were determined via RT‑qPCR and western blotting. Data analysis was performed using R software. miR‑29c expression was significantly downregulated in the lungs of silicotic rats and in the pulmonary fibroblasts of the in vitro model of silicosis. Furthermore, COL1α1, COL3α1, α‑SMA and TGF‑β1 expression levels were significantly increased in cultured fibroblasts following 12 or 18 h exposure to SiO2. Lentiviral‑mediated knockdown of miR‑29c resulted in increased the expression levels of COL1α1, COL3α1, α‑SMA and TGF‑β1, while lentiviral‑mediated miR‑29c overexpression significantly suppressed the expression levels of these fibrosis‑related genes. Taken together, these results demonstrated that miR‑29c was significantly associated with silica‑induced pulmonary fibrosis and the expression levels of COL1α1, COL3α1, TGF‑β1 and α‑SMA are under the regulation of miR‑29c to different extents. This study therefore identified possible candidate molecular targets for preventing or delaying the occurrence and progression of silicosis.
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Copy and paste a formatted citation
Spandidos Publications style
Huang L, Zhang Y, Yang J, Li J, Wu J, Wang F, Lan Y and Zhang Q: Anti‑fibrotic effects and the mechanism of action of miR‑29c in silicosis. Mol Med Rep 23: 292, 2021.
APA
Huang, L., Zhang, Y., Yang, J., Li, J., Wu, J., Wang, F. ... Zhang, Q. (2021). Anti‑fibrotic effects and the mechanism of action of miR‑29c in silicosis. Molecular Medicine Reports, 23, 292. https://doi.org/10.3892/mmr.2021.11932
MLA
Huang, L., Zhang, Y., Yang, J., Li, J., Wu, J., Wang, F., Lan, Y., Zhang, Q."Anti‑fibrotic effects and the mechanism of action of miR‑29c in silicosis". Molecular Medicine Reports 23.4 (2021): 292.
Chicago
Huang, L., Zhang, Y., Yang, J., Li, J., Wu, J., Wang, F., Lan, Y., Zhang, Q."Anti‑fibrotic effects and the mechanism of action of miR‑29c in silicosis". Molecular Medicine Reports 23, no. 4 (2021): 292. https://doi.org/10.3892/mmr.2021.11932
Copy and paste a formatted citation
x
Spandidos Publications style
Huang L, Zhang Y, Yang J, Li J, Wu J, Wang F, Lan Y and Zhang Q: Anti‑fibrotic effects and the mechanism of action of miR‑29c in silicosis. Mol Med Rep 23: 292, 2021.
APA
Huang, L., Zhang, Y., Yang, J., Li, J., Wu, J., Wang, F. ... Zhang, Q. (2021). Anti‑fibrotic effects and the mechanism of action of miR‑29c in silicosis. Molecular Medicine Reports, 23, 292. https://doi.org/10.3892/mmr.2021.11932
MLA
Huang, L., Zhang, Y., Yang, J., Li, J., Wu, J., Wang, F., Lan, Y., Zhang, Q."Anti‑fibrotic effects and the mechanism of action of miR‑29c in silicosis". Molecular Medicine Reports 23.4 (2021): 292.
Chicago
Huang, L., Zhang, Y., Yang, J., Li, J., Wu, J., Wang, F., Lan, Y., Zhang, Q."Anti‑fibrotic effects and the mechanism of action of miR‑29c in silicosis". Molecular Medicine Reports 23, no. 4 (2021): 292. https://doi.org/10.3892/mmr.2021.11932
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