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Tetrandrine attenuates ischemia/reperfusion‑induced neuronal damage in the subacute phase

  • Authors:
    • Yu Wang
    • Xinjun Cai
    • Zhiheng Wu
    • Leilei Tang
    • Lingqun Lu
    • Yinyin Xu
    • Xiaogang Bao
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Zhejiang Integrated Traditional and Western Medicine Hospital, Hangzhou, Zhejiang 310003, P.R. China, School of Clinical Medicine, Wannan Medicial College, Wuhu, Anhui 241002, P.R. China, Department of Pharmacy, Xiaoshan Hospital, Hangzhou, Zhejiang 311200, P.R. China, Laboratory Animal Center, Hangzhou Medical College, Hangzhou, Zhejiang 310013, P.R. China, Department of Orthopedic Surgery, Spine Center, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 297
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    Published online on: February 23, 2021
       https://doi.org/10.3892/mmr.2021.11936
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Abstract

Ischemic stroke, the third leading cause of disability globally, imposes a notable economic burden. Tetrandrine (Tet), which has been widely used clinically, exhibits potential protective effects against stroke. However, there has been little pre‑clinical research to evaluate the therapeutic effects of Tet on stroke. The present study investigated the beneficial effect of Tet on ischemia‑reperfusion (I/R) injury and its underlying mechanism in rats. Rats were subjected to occlusion of the middle cerebral artery, then treated with Tet (30 mg/kg/day, intraperitoneal) in the subacute phase for 7 days. In order to detect the effects of Tet on the behavior of rats, modified neurological severity score and longa behavior, grasping capability and inclined plane tests were conducted on days 1, 3 and 7 following cerebral ischemia. In addition, neuronal apoptosis in the cortex and hippocampus following ischemia was assessed by Nissl staining and TUNEL assay. Finally, oxidative stress was evaluated by measurement of free radicals and immunofluorescence staining of LC3 was used to assess autophagy. Tet improved neurological function and decreased infarct volume in I/R injury rats. Tet also prevented neuronal apoptosis in the cortex and hippocampus region. In addition, Tet protected against oxidative damage following ischemia, which was reflected by decreased levels of nitric oxide and malondialdehyde and increased levels of glutathione (GSH) and GSH peroxidase. In addition, the expression levels of the autophagy marker LC3 decreased in the Tet treatment group. In conclusion, Tet attenuated I/R‑induced neuronal damage in the subacute phase by decreasing oxidative stress, apoptosis and autophagy.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Cai X, Wu Z, Tang L, Lu L, Xu Y and Bao X: Tetrandrine attenuates ischemia/reperfusion‑induced neuronal damage in the subacute phase. Mol Med Rep 23: 297, 2021.
APA
Wang, Y., Cai, X., Wu, Z., Tang, L., Lu, L., Xu, Y., & Bao, X. (2021). Tetrandrine attenuates ischemia/reperfusion‑induced neuronal damage in the subacute phase. Molecular Medicine Reports, 23, 297. https://doi.org/10.3892/mmr.2021.11936
MLA
Wang, Y., Cai, X., Wu, Z., Tang, L., Lu, L., Xu, Y., Bao, X."Tetrandrine attenuates ischemia/reperfusion‑induced neuronal damage in the subacute phase". Molecular Medicine Reports 23.4 (2021): 297.
Chicago
Wang, Y., Cai, X., Wu, Z., Tang, L., Lu, L., Xu, Y., Bao, X."Tetrandrine attenuates ischemia/reperfusion‑induced neuronal damage in the subacute phase". Molecular Medicine Reports 23, no. 4 (2021): 297. https://doi.org/10.3892/mmr.2021.11936
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Cai X, Wu Z, Tang L, Lu L, Xu Y and Bao X: Tetrandrine attenuates ischemia/reperfusion‑induced neuronal damage in the subacute phase. Mol Med Rep 23: 297, 2021.
APA
Wang, Y., Cai, X., Wu, Z., Tang, L., Lu, L., Xu, Y., & Bao, X. (2021). Tetrandrine attenuates ischemia/reperfusion‑induced neuronal damage in the subacute phase. Molecular Medicine Reports, 23, 297. https://doi.org/10.3892/mmr.2021.11936
MLA
Wang, Y., Cai, X., Wu, Z., Tang, L., Lu, L., Xu, Y., Bao, X."Tetrandrine attenuates ischemia/reperfusion‑induced neuronal damage in the subacute phase". Molecular Medicine Reports 23.4 (2021): 297.
Chicago
Wang, Y., Cai, X., Wu, Z., Tang, L., Lu, L., Xu, Y., Bao, X."Tetrandrine attenuates ischemia/reperfusion‑induced neuronal damage in the subacute phase". Molecular Medicine Reports 23, no. 4 (2021): 297. https://doi.org/10.3892/mmr.2021.11936
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