Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
May-2021 Volume 23 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2021 Volume 23 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Expression of immunoglobulin G in human proximal tubular epithelial cells

  • Authors:
    • Zhenling Deng
    • Ziyang Jing
    • Yanhong Guo
    • Junfan Ma
    • Huige Yan
    • Zhan Shi
    • Hui Deng
    • Yaoxian Liang
    • Song Wang
    • Zhuan Cui
    • Yuejuan Pan
    • Xiaoyan Qiu
    • Yue Wang
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Peking University Third Hospital, Beijing 100191, P.R. China, Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, P.R. China
    Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 327
    |
    Published online on: March 8, 2021
       https://doi.org/10.3892/mmr.2021.11966
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Proximal tubular epithelial cells (PTECs) have innate immune characteristics, and produce proinflammatory factors, chemokines and complement components that drive epithelial‑mesenchymal transition (EMT). Our previous studies revealed that human mesangial cells and podocytes were able to synthesize and secrete immunoglobulin (Ig)A and IgG, respectively. The aim of the present study was to evaluate the expression of Igs in PTECs. Firstly, IgG was detected in the cytoplasm, the cell membrane and the lumen of PTECs in the normal renal cortex by immunohistochemistry. Secondly, Igγ gene transcription and V(D)J recombination were detected in single PTECs by nested PCR and Sanger sequencing. Thirdly, Igγ, Igκ and Igλ were clearly detected in an immortalized PTEC line (HK‑2) by immunostaining and western blotting, in which RP215 (an antibody that predominantly binds to non‑B cell‑derived IgG) was used. In addition, Igγ, Igκ and Igλ gene transcripts, conservative V(D)J recombination in the Igγ variable region, recombination activating gene 1/2 and activation‑induced cytidine deaminase were all detected in HK‑2 cells. These data suggested that PTECs may express IgG in a similar manner to B cells. Furthermore, IgG expression was upregulated by TGF‑β1 and may be involved in EMT.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Schlondorff DO: Overview of factors contributing to the pathophysiology of progressive renal disease. Kidney Int. 74:860–866. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Donadio ME, Loiacono E, Peruzzi L, Amore A, Camilla R, Chiale F, Vergano L, Boido A, Conrieri M, Bianciotto M, et al: Toll-like receptors, immunoproteasome and regulatory T cells in children with Henoch-Schonlein purpura and primary IgA nephropathy. Pediatr Nephrol. 29:1545–1551. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Breda PC, Wiech T, Meyer-Schwesinger C, Grahammer F, Huber T, Panzer U, Tiegs G and Neumann K: Renal proximal tubular epithelial cells exert immunomodulatory function by driving inflammatory CD4+ T cell responses. Am J Physiol Renal Physiol. 317:F77–F89. 2019. View Article : Google Scholar : PubMed/NCBI

4 

Liu BC, Tang TT, Lv LL and Lan HY: Renal tubule injury: A driving force toward chronic kidney disease. Kidney Int. 93:568–579. 2018. View Article : Google Scholar : PubMed/NCBI

5 

Kobayashi N, Suzuki Y, Tsuge T, Okumura K, Ra C and Tomino Y: FcRn-mediated transcytosis of immunoglobulin G in human renal proximal tubular epithelial cells. Am J Physiol Renal Physiol. 282:F358–F365. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Qiu X, Zhu X, Zhang L, Mao Y, Zhang J, Hao P, Li G, Lv P, Li Z, Sun X, et al: Human epithelial cancers secrete immunoglobulin g with unidentified specificity to promote growth and survival of tumor cells. Cancer Res. 63:6488–6495. 2003.PubMed/NCBI

7 

Zheng J, Huang J, Mao Y, Liu S, Sun X, Zhu X, Ma T, Zhang L, Ji J, Zhang Y, et al: Immunoglobulin gene transcripts have distinct VHDJH recombination characteristics in human epithelial cancer cells. J Biol Chem. 284:13610–13619. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Jiang D, Ge J, Liao Q, Ma J, Liu Y, Huang J, Wang C, Xu W, Zheng J, Shao W, et al: IgG and IgA with potential microbial-binding activity are expressed by normal human skin epidermal cells. Int J Mol Sci. 16:2574–2590. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Zhu Z, Zhang M, Shao W, Wang P, Gong X, Ma J, Qiu X and Wang B: Immunoglobulin M, a novel molecule of myocardial cells of mice. Int J Biochem Cell Biol. 88:172–180. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Niu N, Zhang J, Sun Y, Wang S, Sun Y, Korteweg C, Gao W and Gu J: Expression and distribution of immunoglobulin G and its receptors in an immune privileged site: The eye. Cell Mol Life Sci. 68:2481–2492. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Huang J, Sun X, Mao Y, Zhu X, Zhang P, Zhang L, Du J and Qiu XY: Expression of immunoglobulin gene with classical V-(D)-J rearrangement in mouse brain neurons. Int J Biochem Cell Biol. 40:1604–1615. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Niu N, Zhang J, Guo Y, Zhao Y, Korteweg C and Gu J: Expression and distribution of immunoglobulin G and its receptors in the human nervous system. Int J Biochem Cell Biol. 43:556–563. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Li J, Korteweg C, Qiu Y, Luo J, Chen Z, Huang G, Li W and Gu J: Two ultrastructural distribution patterns of immunoglobulin G in human placenta and functional implications. Biol Reprod. 91:1282014. View Article : Google Scholar : PubMed/NCBI

14 

Huang J, Zhang L, Ma T, Zhang P and Qiu X: Expression of immunoglobulin gene with classical V-(D)-J rearrangement in mouse testis and epididymis. J Histochem Cytochem. 57:339–349. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Tang J, Zhang J, Liu Y, Liao Q, Huang J, Geng Z, Xu W, Sheng Z, Lee G, Zhang Y, et al: Lung squamous cell carcinoma cells express non-canonically glycosylated IgG that activates integrin-FAK signaling. Cancer Lett. 430:148–159. 2018. View Article : Google Scholar : PubMed/NCBI

16 

Cui M, You L, Zheng B, Huang X, Liu QF, Huang J, Pan B, Qiu X, Liao Q and Zhao Y: High expression of cancer-derived glycosylated immunoglobulin G predicts poor prognosis in pancreatic ductal adenocarcinoma. J Cancer. 11:2213–2221. 2020. View Article : Google Scholar : PubMed/NCBI

17 

Cui M, Hu Y, Zheng B, Zhang S, Zhang X, Wang M, Qiu XY, Liao Q and Zhao YP: Cancer-derived immunoglobulin G: A novel marker for differential diagnosis and relapse prediction in parathyroid carcinoma. Clin Endocrinol (Oxf). 92:461–467. 2020. View Article : Google Scholar : PubMed/NCBI

18 

Deng H, Ma J, Jing Z, Deng Z, Liang Y, A L, Liu Y, Qiu X and Wang Y: Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion. Mol Med Rep. 17:5272–5282. 2018.PubMed/NCBI

19 

Jing Z, Deng H, Ma J, Guo Y, Liang Y, Wu R, A L, Geng Z, Qiu X and Wang Y: Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion. Int J Mol Med. 41:3296–3306. 2018.PubMed/NCBI

20 

Van der Hauwaert C, Savary G, Gnemmi V, Glowacki F, Pottier N, Bouillez A, Maboudou P, Zini L, Leroy X, Cauffiez C, et al: Isolation and characterization of a primary proximal tubular epithelial cell model from human kidney by CD10/CD13 double labeling. PLoS One. 8:e667502013. View Article : Google Scholar : PubMed/NCBI

21 

Tang F, Barbacioru C, Nordman E, Li B, Xu N, Bashkirov VI, Lao K and Surani MA: RNA-Seq analysis to capture the transcriptome landscape of a single cell. Nat Protoc. 5:516–535. 2010. View Article : Google Scholar : PubMed/NCBI

22 

van Dongen JJ, Langerak AW, Bruggemann M, Evans PA, Hummel M, Lavender FL, Delabesse E, Davi F, Schuuring E, García-Sanz R, et al: Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 concerted action BMH4-CT98-3936. Leukemia. 17:2257–2317. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Chen Z and Gu J: Immunoglobulin G expression in carcinomas and cancer cell lines. FASEB J. 21:2931–2938. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Crotty S: Follicular helper CD4 T cells (TFH). Annu Rev Immunol. 29:621–663. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Coffman RL, Lebman DA and Shrader B: Transforming growth factor beta specifically enhances IgA production by lipopolysaccharide-stimulated murine B lymphocytes. J Exp Med 170: 1039, 1989. J Immunol. 182:8–13. 2009.PubMed/NCBI

26 

van Vlasselaer P, Punnonen J and de Vries JE: Transforming growth factor-beta directs IgA switching in human B cells. J Immunol. 148:2062–2067. 1992.PubMed/NCBI

27 

McIntyre TM, Klinman DR, Rothman P, Lugo M, Dasch JR, Mond JJ and Snapper CM: Transforming growth factor beta 1 selectively stimulates immunoglobulin G2b secretion by lipopolysaccharide-activated murine B cells. J Exp Med. 177:1031–1037. 1993. View Article : Google Scholar : PubMed/NCBI

28 

Duan Z, Deng H, Xu S, Jiang Y, Liu H, Li M, Hu D, Li W, Bode AM, Dong Z and Cao Y: Activation of the Ig Iα1 promoter by the transcription factor Ets-1 triggers Ig Iα1-Cα1 germline transcription in epithelial cancer cells. Cell Mol Immunol. 11:197–205. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Peng J, Wang HC, Liu Y, Jiang JH, Lv WQ, Yang Y, Li CY and Qiu XY: Involvement of non-B cell-derived immunoglobulin G in the metastasis and prognosis of salivary adenoid cystic carcinoma. Oncol Lett. 14:4491–4498. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Jiang C, Huang T, Wang Y, Huang G, Wan X and Gu J: Immunoglobulin G expression in lung cancer and its effects on metastasis. PLoS One. 9:e973592014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Deng Z, Jing Z, Guo Y, Ma J, Yan H, Shi Z, Deng H, Liang Y, Wang S, Cui Z, Cui Z, et al: Expression of immunoglobulin G in human proximal tubular epithelial cells. Mol Med Rep 23: 327, 2021.
APA
Deng, Z., Jing, Z., Guo, Y., Ma, J., Yan, H., Shi, Z. ... Wang, Y. (2021). Expression of immunoglobulin G in human proximal tubular epithelial cells. Molecular Medicine Reports, 23, 327. https://doi.org/10.3892/mmr.2021.11966
MLA
Deng, Z., Jing, Z., Guo, Y., Ma, J., Yan, H., Shi, Z., Deng, H., Liang, Y., Wang, S., Cui, Z., Pan, Y., Qiu, X., Wang, Y."Expression of immunoglobulin G in human proximal tubular epithelial cells". Molecular Medicine Reports 23.5 (2021): 327.
Chicago
Deng, Z., Jing, Z., Guo, Y., Ma, J., Yan, H., Shi, Z., Deng, H., Liang, Y., Wang, S., Cui, Z., Pan, Y., Qiu, X., Wang, Y."Expression of immunoglobulin G in human proximal tubular epithelial cells". Molecular Medicine Reports 23, no. 5 (2021): 327. https://doi.org/10.3892/mmr.2021.11966
Copy and paste a formatted citation
x
Spandidos Publications style
Deng Z, Jing Z, Guo Y, Ma J, Yan H, Shi Z, Deng H, Liang Y, Wang S, Cui Z, Cui Z, et al: Expression of immunoglobulin G in human proximal tubular epithelial cells. Mol Med Rep 23: 327, 2021.
APA
Deng, Z., Jing, Z., Guo, Y., Ma, J., Yan, H., Shi, Z. ... Wang, Y. (2021). Expression of immunoglobulin G in human proximal tubular epithelial cells. Molecular Medicine Reports, 23, 327. https://doi.org/10.3892/mmr.2021.11966
MLA
Deng, Z., Jing, Z., Guo, Y., Ma, J., Yan, H., Shi, Z., Deng, H., Liang, Y., Wang, S., Cui, Z., Pan, Y., Qiu, X., Wang, Y."Expression of immunoglobulin G in human proximal tubular epithelial cells". Molecular Medicine Reports 23.5 (2021): 327.
Chicago
Deng, Z., Jing, Z., Guo, Y., Ma, J., Yan, H., Shi, Z., Deng, H., Liang, Y., Wang, S., Cui, Z., Pan, Y., Qiu, X., Wang, Y."Expression of immunoglobulin G in human proximal tubular epithelial cells". Molecular Medicine Reports 23, no. 5 (2021): 327. https://doi.org/10.3892/mmr.2021.11966
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team