Expression of immunoglobulin G in human proximal tubular epithelial cells

Deng,Zhenling; Jing,Ziyang; Guo,Yanhong; Ma,Junfan; Yan,Huige; Shi,Zhan; Deng,Hui; Liang,Yaoxian; Wang,Song; Cui,Zhuan; Pan,Yuejuan; Qiu,Xiaoyan; Wang,Yue

doi:10.3892/mmr.2021.11966

Expression of immunoglobulin G in human proximal tubular epithelial cells

Authors:
- Zhenling Deng
- Ziyang Jing
- Yanhong Guo
- Junfan Ma
- Huige Yan
- Zhan Shi
- Hui Deng
- Yaoxian Liang
- Song Wang
- Zhuan Cui
- Yuejuan Pan
- Xiaoyan Qiu
- Yue Wang
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Affiliations: Department of Nephrology, Peking University Third Hospital, Beijing 100191, P.R. China, Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, P.R. China
Published online on: March 8, 2021 https://doi.org/10.3892/mmr.2021.11966
Article Number: 327
Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Proximal tubular epithelial cells (PTECs) have innate immune characteristics, and produce proinﬂammatory factors, chemokines and complement components that drive epithelial‑mesenchymal transition (EMT). Our previous studies revealed that human mesangial cells and podocytes were able to synthesize and secrete immunoglobulin (Ig)A and IgG, respectively. The aim of the present study was to evaluate the expression of Igs in PTECs. Firstly, IgG was detected in the cytoplasm, the cell membrane and the lumen of PTECs in the normal renal cortex by immunohistochemistry. Secondly, Igγ gene transcription and V(D)J recombination were detected in single PTECs by nested PCR and Sanger sequencing. Thirdly, Igγ, Igκ and Igλ were clearly detected in an immortalized PTEC line (HK‑2) by immunostaining and western blotting, in which RP215 (an antibody that predominantly binds to non‑B cell‑derived IgG) was used. In addition, Igγ, Igκ and Igλ gene transcripts, conservative V(D)J recombination in the Igγ variable region, recombination activating gene 1/2 and activation‑induced cytidine deaminase were all detected in HK‑2 cells. These data suggested that PTECs may express IgG in a similar manner to B cells. Furthermore, IgG expression was upregulated by TGF‑β1 and may be involved in EMT.

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