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The effect of anti‑HLA class I antibodies on the immunological properties of human glomerular endothelial cells and their modification by mTOR inhibition or GCN2 kinase activation

  • Authors:
    • Theodoros Eleftheriadis
    • Georgios Pissas
    • Marta Crespo
    • Georgios Filippidis
    • Nikolaos Antoniadis
    • Vassilios Liakopoulos
    • Ioannis Stefanidis
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Faculty of Medicine, University of Thessaly, Larissa 41110, Greece, Nephrology Department, Hospital del Mar, Mar Health Park, Hospital del Mar Medical Research Institute, Barcelona 08003, Spain, Organ Transplant Unit, Faculty of Medicine, Aristotle University of Thessaloniki, Thessaloniki 54642, Greece
    Copyright: © Eleftheriadis et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 355
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    Published online on: March 12, 2021
       https://doi.org/10.3892/mmr.2021.11994
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Abstract

In antibody‑mediated rejection (ABMR), the graft endothelium is at the forefront of the kidney transplant against the assault from the recipient's humoral immune system, and is a target of the latter. The present study investigated the effect of antibodies against human leukocyte antigen (HLA) class I (anti‑HLAI) on the immunological properties of human glomerular endothelial cells. Additionally, the effect of the mammalian target of rapamycin (mTOR) complex 1 (mTORC1) inhibitor (everolimus), or the general control nonderepressible 2 kinase (GCN2K) activator (halofuginone) on anti‑HLAI antibody‑mediated alterations was assessed. Cell integrity was examined, an lactate dehydrogenase (LDH) release assay was performed and cleaved caspase‑3 levels were determined. Furthermore, cell proliferation was analyzed by performing a bromodeoxyuridine assay and the cellular proteins involved in signal transduction or immune effector mechanisms were assessed via western blotting. IL‑8, monocyte chemoattractive protein‑1 (MCP‑1), von Willebrand factor (vWF) and transforming growth factor‑beta 1 (TGF‑β1) were assayed via ELISA. The results revealed that anti‑HLAI triggered integrin signaling, activated mTOR and GCN2K, preserved cell integrity and promoted cell proliferation. Additionally, by increasing intercellular adhesion molecule 1 (ICAM‑1), HLA‑DR, IL‑8 and MCP‑1 levels, anti‑HLAI enhanced the ability of immune cells to interact with endothelial cells thus facilitating graft rejection. Contrarily, by upregulating CD46 and CD59, anti‑HLAI rendered the endothelium less vulnerable to complement‑mediated injury. Finally, by enhancing vWF and TGF‑β1, anti‑HLAI may render the endothelium prothrombotic and facilitate fibrosis and graft failure, respectively. According to our results, mTORC1 inhibition and GCN2K activation may prove useful pharmaceutical targets, as they prevent cell proliferation and downregulate ICAM‑1, IL‑8, MCP‑1 and TGF‑β1. mTORC1 inhibition also decreases vWF.
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View References

1 

Sellares J, de Freitas DG, Mengel M, Reeve J, Einecke G, Sis B, Hidalgo LG, Famulski K, Matas A and Halloran PF: Understanding the causes of kidney transplant failure: The dominant role of antibody-mediated rejection and nonadherence. Am J Transplant. 12:388–399. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Abramowicz D, Oberbauer R, Heemann U, Viklicky O, Peruzzi L, Mariat C, Crespo M, Budde K and Oniscu GC: Recent advances in kidney transplantation: A viewpoint from the descartes advisory board. Nephrol Dial Transplant. 33:1699–1707. 2018. View Article : Google Scholar : PubMed/NCBI

3 

Loupy A and Lefaucheur C: Antibody-mediated rejection of solid-organ allografts. N Engl J Med. 379:1150–1160. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Muczynski KA, Ekle DM, Coder DM and Anderson SK: Normal human kidney HLA-DR-expressing renal microvascular endothelial cells: Characterization, isolation, and regulation of MHC class II expression. J Am Soc Nephrol. 14:1336–1348. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Thomas KA, Valenzuela NM and Reed EF: The perfect storm: HLA antibodies, complement, FcγRs, and endothelium in transplant rejection. Trends Mol Med. 21:319–329. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Katsuma A, Yamakawa T, Nakada Y, Yamamoto I and Yokoo T: Histopathological findings in transplanted kidneys. Renal Replacement Therapy. 3:62017. View Article : Google Scholar

7 

Jindra PT, Jin YP, Rozengurt E and Reed EF: HLA class I antibody-mediated endothelial cell proliferation via the mTOR pathway. J Immunol. 180:2357–2366. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Jin YP, Valenzuela NM, Ziegler ME, Rozengurt E and Reed EF: Everolimus inhibits Anti-HLA I antibody-mediated endothelial cell signaling, migration and proliferation more potently than sirolimus. Am J Transplant. 14:806–819. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Salehi S, Sosa RA, Jin YP, Kageyama S, Fishbein MC, Rozengurt E, Kupiec-Weglinski JW and Reed EF: Outside-in HLA class I signaling regulates ICAM-1 clustering and endothelial cell-monocyte interactions via mTOR in transplant antibody-mediated rejection. Am J Transplant. 18:1096–1109. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Li F, Rao P, Hong L, Fishbein MC, Gjertson DW and Reed EF: OR49 effect of everolimus immunotherapy on HLA-antibody mediated activation of endothelial cells in heart transplantation. Hum Immunol. 78:462017. View Article : Google Scholar

11 

Laplante M and Sabatini DM: mTOR signaling at a glance. J Cell Sci. 122:3589–3594. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Saran U, Foti M and Dufour JF: Cellular and molecular effects of the mTOR inhibitor everolimus. Clin Sci (Lond). 129:895–914. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Castilho BA, Shanmugam R, Silva RC, Ramesh R, Himme BM and Sattlegger E: Keeping the eIF2 alpha kinase Gcn2 in check. Biochim Biophys Acta. 1843:1948–1968. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Munn DH, Sharma MD, Baban B, Harding HP, Zhang Y, Ron D and Mellor AL: GCN2 kinase in T cells mediates proliferative arrest and anergy induction in response to indoleamine 2,3-dioxygenase. Immunity. 22:633–642. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Eleftheriadis T, Pissas G, Antoniadi G, Spanoulis A, Liakopoulos V and Stefanidis I: Indoleamine 2,3-dioxygenase increases p53 levels in alloreactive human T cells, and both indoleamine 2,3-dioxygenase and p53 suppress glucose uptake, glycolysis and proliferation. Int Immunol. 26:673–684. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Eleftheriadis T, Pissas G, Antoniadi G, Liakopoulos V and Stefanidis I: Indoleamine 2,3-dioxygenase depletes tryptophan, activates general control non-derepressible 2 kinase and down-regulates key enzymes involved in fatty acid synthesis in primary human CD4+ T cells. Immunology. 146:292–300. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Pines M and Spector I: Halofuginone-the multifaceted molecule. Molecules. 20:573–594. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Sundrud MS, Koralov SB, Feuerer M, Calado DP, Kozhaya AE, Rhule-Smith A, Lefebvre RE, Unutmaz D, Mazitschek R, Waldner H, et al: Halofuginone inhibits TH17 cell differentiation by activating the amino acid starvation response. Science. 324:1334–1338. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Carlson TJ, Pellerin A, Djuretic IM, Trivigno C, Koralov SB, Rao A and Sundrud MS: Halofuginone-induced amino acid starvation regulates Stat3-dependent Th17 effector function and reduces established autoimmune inflammation. J Immunol. 192:2167–2176. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Yamakuchi M, Kirkiles-Smith NC, Ferlito M, Cameron SJ, Bao C, Fox-Talbot K, Wasowska BA, Baldwin WM III, Pober JS and Lowenstein CJ: Antibody to human leukocyte antigen triggers endothelial exocytosis. Proc Natl Acad Sci USA. 104:1301–1306. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Naemi FM, Carter V, Kirby JA and Ali S: Anti-donor HLA class I antibodies: Pathways to endothelial cell activation and cell-mediated allograft rejection. Transplantation. 96:258–266. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Jin YP, Singh RP, Du ZY, Rajasekaran AK, Rozengurt E and Reed EF: Ligation of HLA class I molecules on endothelial cells induces phosphorylation of Src, paxillin, and focal adhesion kinase in an actin-dependent manner. J Immunol. 168:5415–5423. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Rostaing L, Christiaans MH, Kovarik JM and Pascual J: The pharmacokinetics of everolimus in de novo kidney transplant patients receiving tacrolimus: An analysis from the randomized ASSET study. Ann Transplant. 19:337–345. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Fadeel B and Orrenius S: Apoptosis: A basic biological phenomenon with wide-ranging implications in human disease. J Intern Med. 258:479–517. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Zhang X, Rozengurt E and Reed EF: HLA class I molecules partner with integrin β4 to stimulate endothelial cell proliferation and migration. Sci Signal. 3:ra852010. View Article : Google Scholar : PubMed/NCBI

26 

Liu L, Chen L, Chung J and Huang S: Rapamycin inhibits F-actin reorganization and phosphorylation of focal adhesion proteins. Oncogene. 27:4998–5010. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Drolet MC, Desbiens-Brassard V, Roussel E, Tu V, Couet J and Arsenault M: Blockade of the acute activation of mTOR complex 1 decreases hypertrophy development in rats with severe aortic valve regurgitation. Springerplus. 4:4352015. View Article : Google Scholar : PubMed/NCBI

28 

Jiao Y, Li G, Li Q, Ali R, Qin L, Li W, Qyang Y, Greif DM, Geirsson A, Humphrey JD and Tellides G: mTOR (Mechanistic Target of Rapamycin) inhibition decreases mechanosignaling, collagen accumulation, and stiffening of the thoracic aorta in elastin-deficient mice. Arterioscler Thromb Vasc Biol. 37:1657–1666. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Lee FY, Zhen YY, Yuen CM, Fan R, Chen YT, Sheu JJ, Chen YL, Wang CJ, Sun CK and Yip HK: The mTOR-FAK mechanotransduction signaling axis for focal adhesion maturation and cell proliferation. Am J Transl Res. 9:1603–1617. 2017.PubMed/NCBI

30 

Sarbassov DD: Phosphorylation and regulation of Akt/PKB by the Rictor-mTOR complex. Science. 307:1098–1101. 2005. View Article : Google Scholar : PubMed/NCBI

31 

Jacinto E, Facchinetti V, Liu D, Soto N, Wei S, Jung SY, Huang Q, Qin J and Su B: SIN1/MIP1 Maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell. 127:125–137. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Silva RC, Sattlegger E and Castilho BA: Perturbations in actin dynamics reconfigure protein complexes that modulate GCN2 activity and promote an eIF2 response. J Cell Sci. 129:4521–4533. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Yang L, Froio RM, Sciuto TE, Dvorak AM, Alon R and Luscinskas FW: ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF-alpha-activated vascular endothelium under flow. Blood. 106:584–592. 2005. View Article : Google Scholar : PubMed/NCBI

34 

Kaizuka Y, Douglass AD, Varma R, Dustin ML and Vale RD: Mechanisms for segregating T cell receptor and adhesion molecules during immunological synapse formation in Jurkat T cells. Proc Natl Acad Sci USA. 104:20296–20301. 2007. View Article : Google Scholar : PubMed/NCBI

35 

Kuokkanen E, Šuštar V and Mattila PK: Molecular control of B cell activation and immunological synapse formation. Traffic. 16:311–326. 2015. View Article : Google Scholar : PubMed/NCBI

36 

Barber DF, Faure M and Long EO: LFA-1 contributes an early signal for NK cell cytotoxicity. J Immunol. 173:3653–3659. 2004. View Article : Google Scholar : PubMed/NCBI

37 

Hsu HT and Orange JS: Distinct integrin-dependent signals define requirements for lytic granule convergence and polarization in natural killer cells. Sci Signal. 7:pe242014. View Article : Google Scholar : PubMed/NCBI

38 

Valenzuela NM, Mulder A and Reed EF: HLA class I antibodies trigger increased adherence of monocytes to endothelial cells by eliciting an increase in endothelial P-selectin and, depending on subclass, by engaging FcγRs. J Immunol. 190:6635–6650. 2013. View Article : Google Scholar : PubMed/NCBI

39 

Neefjes J, Jongsma ML, Paul P and Bakke O: Towards a systems understanding of MHC class I and MHC class II antigen presentation. Nat Rev Immunol. 11:823–836. 2011. View Article : Google Scholar : PubMed/NCBI

40 

Bentall A, Cornell LD, Gloor JM, Park WD, Gandhi MJ, Winters JL, Chedid MF, Dean PG and Stegall MD: Five-year outcomes in living donor kidney transplants with a positive crossmatch. Am J Transplant. 13:76–85. 2013. View Article : Google Scholar : PubMed/NCBI

41 

Mestas J and Hughes CC: Of mice and not men: Differences between mouse and human immunology. J Immunol. 172:2731–2738. 2004. View Article : Google Scholar : PubMed/NCBI

42 

Hayry P, von Willebrand E and Andersson LC: Expression of HLA-ABC and -DR locus antigens on human kidney, endothelial, tubular and glomerular cells. Scand J Immunol. 11:303–310. 1980. View Article : Google Scholar : PubMed/NCBI

43 

Evans PR, Trickett LP, Smith JL, MacIver AG, Tate D and Slapak M: Varying expression of major histocompatibility complex antigens on human renal endothelium and epithelium. Br J Exp Pathol. 66:79–87. 1985.PubMed/NCBI

44 

Daar AS, Fuggle SV, Fabre JW, Ting A and Morris PJ: The detailed distribution of mhc class II antigens in normal human organs. Transplantation. 38:293–298. 1984. View Article : Google Scholar : PubMed/NCBI

45 

Muczynski KA, Cotner T and Anderson SK: Unusual expression of human lymphocyte antigen class II in normal renal microvascular endothelium. Kidney Int. 59:488–497. 2001. View Article : Google Scholar : PubMed/NCBI

46 

McDouall RM, Yacoub M and Rose ML: Isolation, culture, and characterisation of MHC class II-positive microvascular endothelial cells from the human heart. Microvasc Res. 51:137–152. 1996. View Article : Google Scholar : PubMed/NCBI

47 

Orandi BJ, Alachkar N, Kraus ES, Naqvi F, Lonze BE, Lees L, Van Arendonk KJ, Wickliffe C, Bagnasco SM, Zachary AA, et al: Presentation and outcomes of C4d-negative antibody-mediated rejection after kidney transplantation. Am J Transplant. 16:213–220. 2015. View Article : Google Scholar : PubMed/NCBI

48 

Loupy A, Lefaucheur C, Vernerey D, Prugger C, Duong van Huyen JP, Mooney N, Suberbielle C, Frémeaux-Bacchi V, Méjean A, Desgrandchamps F, et al: Complement-binding anti-HLA antibodies and kidney-allograft survival. N Engl J Med. 369:1215–1226. 2013. View Article : Google Scholar : PubMed/NCBI

49 

Merle NS, Church SE, Fremeaux-Bacchi V and Roumenina LT: Complement System Part I-molecular mechanisms of activation and regulation. Front Immunol. 6:2622015. View Article : Google Scholar : PubMed/NCBI

50 

Matsushima K, Baldwin ET and Mukaida N: Interleukin-8 and MCAF: Novel leukocyte recruitment and activating cytokines. Chem Immunol. 51:236–265. 1992. View Article : Google Scholar : PubMed/NCBI

51 

Deshmane SL, Kremlev S, Amini S and Sawaya BE: Monocyte chemoattractant Protein-1 (MCP-1): An overview. J Interferon Cytokine Res. 29:313–326. 2009. View Article : Google Scholar : PubMed/NCBI

52 

Meli A, Carter T, McCormack A, Hannah MJ and Rose ML: Antibody alone is not a stimulator of exocytosis of weibel-palade bodies from human endothelial cells. Transplantation. 94:794–801. 2012. View Article : Google Scholar : PubMed/NCBI

53 

Hassan MI, Saxena A and Ahmad F: Structure and function of von Willebrand factor. Blood Coagul Fibrinolysis. 23:11–22. 2012. View Article : Google Scholar : PubMed/NCBI

54 

Prud'homme GJ: Pathobiology of transforming growth factor beta in cancer, fibrosis and immunologic disease, and therapeutic considerations. Lab Invest. 87:1077–1091. 2007. View Article : Google Scholar : PubMed/NCBI

55 

Hart A, Smith JM, Skeans MA, Gustafson SK, Stewart DE, Cherikh WS, Wainright JL, Boyle G, Snyder JJ, Kasiske BL and Israni AK: Kidney. Am J Transplant. 16 (Suppl 2):S11–S46. 2016. View Article : Google Scholar

56 

Berger SP, Sommerer C, Witzke O, Tedesco H, Chadban S, Mulgaonkar S, Qazi Y, de Fijter JW, Oppenheimer F, Cruzado JM, et al: Two-year outcomes in de novo renal transplant recipients receiving everolimus-facilitated calcineurin inhibitor reduction regimen from the TRANSFORM study. Am J Transplant. 19:3018–3034. 2019. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Eleftheriadis T, Pissas G, Crespo M, Filippidis G, Antoniadis N, Liakopoulos V and Stefanidis I: The effect of anti‑HLA class I antibodies on the immunological properties of human glomerular endothelial cells and their modification by mTOR inhibition or GCN2 kinase activation. Mol Med Rep 23: 355, 2021.
APA
Eleftheriadis, T., Pissas, G., Crespo, M., Filippidis, G., Antoniadis, N., Liakopoulos, V., & Stefanidis, I. (2021). The effect of anti‑HLA class I antibodies on the immunological properties of human glomerular endothelial cells and their modification by mTOR inhibition or GCN2 kinase activation. Molecular Medicine Reports, 23, 355. https://doi.org/10.3892/mmr.2021.11994
MLA
Eleftheriadis, T., Pissas, G., Crespo, M., Filippidis, G., Antoniadis, N., Liakopoulos, V., Stefanidis, I."The effect of anti‑HLA class I antibodies on the immunological properties of human glomerular endothelial cells and their modification by mTOR inhibition or GCN2 kinase activation". Molecular Medicine Reports 23.5 (2021): 355.
Chicago
Eleftheriadis, T., Pissas, G., Crespo, M., Filippidis, G., Antoniadis, N., Liakopoulos, V., Stefanidis, I."The effect of anti‑HLA class I antibodies on the immunological properties of human glomerular endothelial cells and their modification by mTOR inhibition or GCN2 kinase activation". Molecular Medicine Reports 23, no. 5 (2021): 355. https://doi.org/10.3892/mmr.2021.11994
Copy and paste a formatted citation
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Spandidos Publications style
Eleftheriadis T, Pissas G, Crespo M, Filippidis G, Antoniadis N, Liakopoulos V and Stefanidis I: The effect of anti‑HLA class I antibodies on the immunological properties of human glomerular endothelial cells and their modification by mTOR inhibition or GCN2 kinase activation. Mol Med Rep 23: 355, 2021.
APA
Eleftheriadis, T., Pissas, G., Crespo, M., Filippidis, G., Antoniadis, N., Liakopoulos, V., & Stefanidis, I. (2021). The effect of anti‑HLA class I antibodies on the immunological properties of human glomerular endothelial cells and their modification by mTOR inhibition or GCN2 kinase activation. Molecular Medicine Reports, 23, 355. https://doi.org/10.3892/mmr.2021.11994
MLA
Eleftheriadis, T., Pissas, G., Crespo, M., Filippidis, G., Antoniadis, N., Liakopoulos, V., Stefanidis, I."The effect of anti‑HLA class I antibodies on the immunological properties of human glomerular endothelial cells and their modification by mTOR inhibition or GCN2 kinase activation". Molecular Medicine Reports 23.5 (2021): 355.
Chicago
Eleftheriadis, T., Pissas, G., Crespo, M., Filippidis, G., Antoniadis, N., Liakopoulos, V., Stefanidis, I."The effect of anti‑HLA class I antibodies on the immunological properties of human glomerular endothelial cells and their modification by mTOR inhibition or GCN2 kinase activation". Molecular Medicine Reports 23, no. 5 (2021): 355. https://doi.org/10.3892/mmr.2021.11994
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