Open Access

MicroRNA-124 facilitates lens epithelial cell apoptosis by inhibiting SPRY2 and MMP-2

  • Authors:
    • Yan Liu
    • Shuting Li
    • Yao Liu
    • Xujing Lv
    • Qing Zhou
  • View Affiliations

  • Published online on: March 17, 2021     https://doi.org/10.3892/mmr.2021.12020
  • Article Number: 381
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Age-related cataract (ARC) is the primary cause of blindness worldwide. Abnormal expression of microRNAs (miRNAs/miRs) has been reported to be associated with multiple diseases, including ARC. However, the potential role of miR-124 in ARC remains unclear. The present study used the human lens epithelial cell line, SRA01/04, to investigate the potential role of miR-124 in ARC. Reverse transcription-quantitative PCR analysis was performed to detect the expression levels of miR-124, protein sprouty homolog 2 (SPRY2) and matrix metalloproteinase-2 (MMP-2) in ARC tissues, while western blotting was performed to detect the protein levels of SPRY2 and MMP-2. Cell viability and apoptosis of SRA01/04 cells were assessed via Cell Counting Kit-8 and TUNEL assays, respectively. The interaction between miR-124 and SPRY2 or MMP-2 was confirmed via the dual-luciferase reporter and RNA immunoprecipitation assays. The results of the present study demonstrated that miR-124 expression was significantly upregulated in ARC tissues, and knockdown of miR-124 increased SRA01/04 cell viability and suppressed apoptosis. In addition, SPRY2 and MMP-2 expression was decreased in ARC tissues, and were demonstrated to directly bind to miR-124. Overexpression of SPRY2 or MMP-2 increased SRA01/04 cell viability and repressed apoptosis, the effects of which were reversed following overexpression of miR-124. Taken together, these results suggested that miR-124 facilitates lens epithelial cell apoptosis by modulating SPRY2 or MMP-2 expression, providing a novel treatment approach for ARC.
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May-2021
Volume 23 Issue 5

Print ISSN: 1791-2997
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Spandidos Publications style
Liu Y, Li S, Liu Y, Lv X and Zhou Q: MicroRNA-124 facilitates lens epithelial cell apoptosis by inhibiting SPRY2 and MMP-2. Mol Med Rep 23: 381, 2021
APA
Liu, Y., Li, S., Liu, Y., Lv, X., & Zhou, Q. (2021). MicroRNA-124 facilitates lens epithelial cell apoptosis by inhibiting SPRY2 and MMP-2. Molecular Medicine Reports, 23, 381. https://doi.org/10.3892/mmr.2021.12020
MLA
Liu, Y., Li, S., Liu, Y., Lv, X., Zhou, Q."MicroRNA-124 facilitates lens epithelial cell apoptosis by inhibiting SPRY2 and MMP-2". Molecular Medicine Reports 23.5 (2021): 381.
Chicago
Liu, Y., Li, S., Liu, Y., Lv, X., Zhou, Q."MicroRNA-124 facilitates lens epithelial cell apoptosis by inhibiting SPRY2 and MMP-2". Molecular Medicine Reports 23, no. 5 (2021): 381. https://doi.org/10.3892/mmr.2021.12020