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Article

Dihydromyricetin inhibits oxidative stress and apoptosis in oxygen and glucose deprivation/reoxygenation‑induced HT22 cells by activating the Nrf2/HO‑1 pathway

  • Authors:
    • Qian Zhang
    • Jia Wang
    • Huili Zhang
    • Tao Zeng
  • View Affiliations / Copyright

    Affiliations: Department of Encephalopathy, Gansu Provincial Hospital of TCM, Lanzhou, Gansu 730050, P.R. China, Department of Rheumatic Osteopathy, Affiliated Hospital of Gansu University of Traditional Chinese Medicine, Lanzhou, Gansu 730000, P.R. China, Department of Neurology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510180, P.R. China
  • Article Number: 397
    |
    Published online on: March 25, 2021
       https://doi.org/10.3892/mmr.2021.12036
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Abstract

Cerebral ischemia‑reperfusion injury (CIRI) refers to the phenomenon that ischemic injury of the brain leads to the injury of brain cells, which is further aggravated after the recovery of blood reperfusion. Dihydromyricetin (DHM) has an effective therapeutic effect on vascular diseases; however, its role in CIRI has not been investigated. The oxygen and glucose deprivation/reoxygenation (OGD/R) cell model was used on HT22 hippocampal neurons in mice, by oxygen and sugar deprivation. DHM was found to increase the cell viability of HT22 cells following OGD/R induction. The levels of malondialdehyde (MDA) decreased, superoxide dismutase (SOD) and glutathione (GSH) in the OGD/R‑induced HT22 cells increased following DHM treatment, accompanied by the decreased protein expression levels of NOX2 and NOX4. DHM also inhibited cell apoptosis induced by OGD/R, and decreased the protein expression levels of Bax and caspase‑3, and increased the expression levels of Bcl‑2. Moreover, the expression levels of the NF‑E2‑related factor 2 (Nrf2)/heme oxygenase (HO‑1) signaling pathway‑associated proteins in OGD/R‑induced HT22 were increased following DHM treatment, and the effect of DHM on oxidative stress and apoptosis was reversed after the addition of the Nrf2/HO‑1 pathway inhibitor, brusatol. In conclusion, DHM inhibited oxidative stress and apoptosis in OGD/R‑induced HT22 cells by activating the Nrf2/HO‑1 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Q, Wang J, Zhang H and Zeng T: Dihydromyricetin inhibits oxidative stress and apoptosis in oxygen and glucose deprivation/reoxygenation‑induced HT22 cells by activating the Nrf2/HO‑1 pathway. Mol Med Rep 23: 397, 2021.
APA
Zhang, Q., Wang, J., Zhang, H., & Zeng, T. (2021). Dihydromyricetin inhibits oxidative stress and apoptosis in oxygen and glucose deprivation/reoxygenation‑induced HT22 cells by activating the Nrf2/HO‑1 pathway. Molecular Medicine Reports, 23, 397. https://doi.org/10.3892/mmr.2021.12036
MLA
Zhang, Q., Wang, J., Zhang, H., Zeng, T."Dihydromyricetin inhibits oxidative stress and apoptosis in oxygen and glucose deprivation/reoxygenation‑induced HT22 cells by activating the Nrf2/HO‑1 pathway". Molecular Medicine Reports 23.6 (2021): 397.
Chicago
Zhang, Q., Wang, J., Zhang, H., Zeng, T."Dihydromyricetin inhibits oxidative stress and apoptosis in oxygen and glucose deprivation/reoxygenation‑induced HT22 cells by activating the Nrf2/HO‑1 pathway". Molecular Medicine Reports 23, no. 6 (2021): 397. https://doi.org/10.3892/mmr.2021.12036
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Q, Wang J, Zhang H and Zeng T: Dihydromyricetin inhibits oxidative stress and apoptosis in oxygen and glucose deprivation/reoxygenation‑induced HT22 cells by activating the Nrf2/HO‑1 pathway. Mol Med Rep 23: 397, 2021.
APA
Zhang, Q., Wang, J., Zhang, H., & Zeng, T. (2021). Dihydromyricetin inhibits oxidative stress and apoptosis in oxygen and glucose deprivation/reoxygenation‑induced HT22 cells by activating the Nrf2/HO‑1 pathway. Molecular Medicine Reports, 23, 397. https://doi.org/10.3892/mmr.2021.12036
MLA
Zhang, Q., Wang, J., Zhang, H., Zeng, T."Dihydromyricetin inhibits oxidative stress and apoptosis in oxygen and glucose deprivation/reoxygenation‑induced HT22 cells by activating the Nrf2/HO‑1 pathway". Molecular Medicine Reports 23.6 (2021): 397.
Chicago
Zhang, Q., Wang, J., Zhang, H., Zeng, T."Dihydromyricetin inhibits oxidative stress and apoptosis in oxygen and glucose deprivation/reoxygenation‑induced HT22 cells by activating the Nrf2/HO‑1 pathway". Molecular Medicine Reports 23, no. 6 (2021): 397. https://doi.org/10.3892/mmr.2021.12036
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