Dioscin inhibits SCC15 cell proliferation via the RASSF1A/MST2/YAP axis

Tian,Hui; Chen,Xiyan; Zhang,Yafei; Wang,Ying; Fu,Xucheng; Gu,Weiting; Wen,Yong

doi:10.3892/mmr.2021.12053

Dioscin inhibits SCC15 cell proliferation via the RASSF1A/MST2/YAP axis

Authors:
- Hui Tian
- Xiyan Chen
- Yafei Zhang
- Ying Wang
- Xucheng Fu
- Weiting Gu
- Yong Wen
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Affiliations: Department of Implantology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China, Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
Published online on: March 30, 2021 https://doi.org/10.3892/mmr.2021.12053
Article Number: 414
Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dioscin, an extract from traditional Chinese herbal plants, displays various biological and pharmacological effects on tumors, including inhibition of cell proliferation and induction of DNA damage. However, the effects of dioscin on oral squamous cell carcinoma (OSCC) cells are not completely understood. The present study aimed to evaluate the impact of dioscin on OSCC cell proliferation. Cell Counting Kit‑8 and 5‑ethynyl‑2'‑deoxyuridine incorporation assays were performed to assess cell proliferation. Flow cytometry was conducted to detect alterations in the cell cycle and cell apoptosis. Western blotting and coimmunoprecipitation were performed to determine protein expression levels. In SCC15 cells, dioscin treatment significantly induced cell cycle arrest, increased apoptosis and inhibited proliferation compared with the control group. Mechanistically, the tumor suppressor protein Ras association domain‑containing protein 1A (RASSF1A) was activated and oncoprotein yes‑associated protein (YAP) was phosphorylated by dioscin. Furthermore, YAP overexpression and knockdown reduced and enhanced the inhibitory effects of dioscin on SCC15 cells, respectively. In summary, the results demonstrated that, compared with the control group, dioscin upregulated RASSF1A expression in OSCC cells, which resulted in YAP phosphorylation, thus weakening its transcriptional coactivation function, enhancing cell cycle arrest and apoptosis, and inhibiting cell proliferation. The present study indicated that dioscin may serve as a therapeutic agent for OSCC.

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