LncRNA NR024118 is downregulated in sepsis and inhibits LPS‑induced apoptosis of cardiomyocytes

Qin,Gang; Wei,Liuqing; Jiang,Fufu; Li,Jiayan; Zhang,Bo; Pan,Dongxu; Lu,Xinglin

doi:10.3892/mmr.2021.12073

June-2021 Volume 23 Issue 6

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June-2021 Volume 23 Issue 6

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LncRNA NR024118 is downregulated in sepsis and inhibits LPS‑induced apoptosis of cardiomyocytes

Authors:
- Gang Qin
- Liuqing Wei
- Fufu Jiang
- Jiayan Li
- Bo Zhang
- Dongxu Pan
- Xinglin Lu
View Affiliations / Copyright

Affiliations: ICU Department, Guangxi Zhuang Autonomous National Hospital, Nanning, Guangxi Zhuang Autonomous Region 530001, P.R. China

Copyright: © Qin et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].
Article Number: 434
|
Published online on: April 8, 2021

https://doi.org/10.3892/mmr.2021.12073
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Abstract

It has been reported that lncRNA‑NR024118 can suppress lipopolysaccharide (LPS)‑induced inflammatory responses, which promote sepsis. The present study aimed to investigate the involvement of NR024118 in sepsis. Research subjects included 82 patients with sepsis without myocardial dysfunction (MD), 35 patients with sepsis with MD and 82 healthy controls. The expression levels of NR024118 in plasma collected from these participants and LPS‑induced AC16 cells were measured by reverse transcription‑quantitative PCR. The expression levels of IL‑16 in these plasma samples and LPS‑induced AC16 cells were measured by ELISA. The correlation between the expression levels of NR024118 and IL‑6 across plasma samples were analyzed by Pearson's correlation coefficient. The action potential duration (APD) was measured at 50 and 90% repolarization. Cell apoptosis was determined by cell apoptosis assay. The expression levels of p‑transcription factor p65 were detected by western blot analysis. NF‑κB activity were analyzed by luciferase reporter assay. It was found that NR024118 was downregulated and IL‑6 was upregulated in the plasma of patients with sepsis. Among patients with sepsis, the individuals with MD exhibited even lower expression levels of NR024118 and higher expression levels of IL‑6. Among patients with sepsis with MD, the expression levels of NR024118 and IL‑6 were inversely correlated. LPS could induce MD to construct the sepsis models based on the increased expression levels of TNF‑α, IL‑1β, IL‑6 and shortened APD by LPS‑mediated induction. Overexpression of NR024118 significantly reduced the secretion of IL‑6 and apoptosis of cardiomyocytes under LPS treatment. Functional studies demonstrated that NR024118 had negative regulation on p65 phosphorylation and NF‑κB activation. NR024118 was suppressed in sepsis and inhibited LPS‑induced apoptosis of cardiomyocytes.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

LncRNA NR024118 is downregulated in sepsis and inhibits LPS‑induced apoptosis of cardiomyocytes

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