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Tryptophan 2, 3‑dioxygenase promotes proliferation, migration and invasion of ovarian cancer cells

  • Authors:
    • Yuemei Zhao
    • Fengxing Tao
    • Jiayu Jiang
    • Lina Chen
    • Jizao Du
    • Xiaoxiao Cheng
    • Qin He
    • Shouhui Zhong
    • Wei Chen
    • Xiaoli Wu
    • Rongying Ou
    • Yunsheng Xu
    • Kai-Fu Tang
  • View Affiliations / Copyright

    Affiliations: Digestive Cancer Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325015, P.R. China, Department of Dermato‑Venereology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325015, P.R. China, Department of Medical Ultrasonics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Department of Gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325015, P.R. China, Department of Gynecology and Obstetrics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325015, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 445
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    Published online on: April 12, 2021
       https://doi.org/10.3892/mmr.2021.12084
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Abstract

Tryptophan 2,3‑dioxygenase (TDO2) is a key rate‑limiting enzyme in the kynurenine pathway and promotes tumor growth and escape from immune surveillance in different types of cancer. The present study aimed to investigate whether TDO2 serves a role in the development of ovarian cancer. Reverse transcription‑quantitative PCR and western blotting were used to detect the expression of TDO2 in different cell lines. The effects of TDO2 overexpression, TDO2 knockdown and TDO2 inhibitor on ovarian cancer cell proliferation, migration and invasion were determined by MTS, colony formation and Transwell assays. The expression of TDO2 in ovarian cancer tissues, normal ovarian tissues and fallopian tube tissues were analyzed using the gene expression data from The Cancer Genome Atlas and Genotype‑Tissue Expression project. Immune cell infiltration in cancer tissues was evaluated using the single sample gene set enrichment analysis algorithm. The present study found that RasV12‑mediated oncogenic transformation was accompanied by the upregulation of TDO2. In addition, it was demonstrated that TDO2 was upregulated in ovarian cancer tissues compared with normal ovarian tissues. TDO2 overexpression promoted proliferation, migration and invasion of ovarian cancer cells, whereas TDO2 knockdown repressed these phenotypes. Treatment with LM10, a TDO2 inhibitor, also repressed the proliferation, migration and invasion of ovarian cancer cells. The present study indicated that TDO2 can be used as a new target for the treatment of ovarian cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Y, Tao F, Jiang J, Chen L, Du J, Cheng X, He Q, Zhong S, Chen W, Wu X, Wu X, et al: Tryptophan 2, 3‑dioxygenase promotes proliferation, migration and invasion of ovarian cancer cells. Mol Med Rep 23: 445, 2021.
APA
Zhao, Y., Tao, F., Jiang, J., Chen, L., Du, J., Cheng, X. ... Tang, K. (2021). Tryptophan 2, 3‑dioxygenase promotes proliferation, migration and invasion of ovarian cancer cells. Molecular Medicine Reports, 23, 445. https://doi.org/10.3892/mmr.2021.12084
MLA
Zhao, Y., Tao, F., Jiang, J., Chen, L., Du, J., Cheng, X., He, Q., Zhong, S., Chen, W., Wu, X., Ou, R., Xu, Y., Tang, K."Tryptophan 2, 3‑dioxygenase promotes proliferation, migration and invasion of ovarian cancer cells". Molecular Medicine Reports 23.6 (2021): 445.
Chicago
Zhao, Y., Tao, F., Jiang, J., Chen, L., Du, J., Cheng, X., He, Q., Zhong, S., Chen, W., Wu, X., Ou, R., Xu, Y., Tang, K."Tryptophan 2, 3‑dioxygenase promotes proliferation, migration and invasion of ovarian cancer cells". Molecular Medicine Reports 23, no. 6 (2021): 445. https://doi.org/10.3892/mmr.2021.12084
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Y, Tao F, Jiang J, Chen L, Du J, Cheng X, He Q, Zhong S, Chen W, Wu X, Wu X, et al: Tryptophan 2, 3‑dioxygenase promotes proliferation, migration and invasion of ovarian cancer cells. Mol Med Rep 23: 445, 2021.
APA
Zhao, Y., Tao, F., Jiang, J., Chen, L., Du, J., Cheng, X. ... Tang, K. (2021). Tryptophan 2, 3‑dioxygenase promotes proliferation, migration and invasion of ovarian cancer cells. Molecular Medicine Reports, 23, 445. https://doi.org/10.3892/mmr.2021.12084
MLA
Zhao, Y., Tao, F., Jiang, J., Chen, L., Du, J., Cheng, X., He, Q., Zhong, S., Chen, W., Wu, X., Ou, R., Xu, Y., Tang, K."Tryptophan 2, 3‑dioxygenase promotes proliferation, migration and invasion of ovarian cancer cells". Molecular Medicine Reports 23.6 (2021): 445.
Chicago
Zhao, Y., Tao, F., Jiang, J., Chen, L., Du, J., Cheng, X., He, Q., Zhong, S., Chen, W., Wu, X., Ou, R., Xu, Y., Tang, K."Tryptophan 2, 3‑dioxygenase promotes proliferation, migration and invasion of ovarian cancer cells". Molecular Medicine Reports 23, no. 6 (2021): 445. https://doi.org/10.3892/mmr.2021.12084
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