Pyropia yezoensis protein protects against TNF‑α‑induced myotube atrophy in C2C12 myotubes via the NF‑κB signaling pathway
- Min-Kyeong Lee
- Youn Hee Choi
- Taek-Jeong Nam
Affiliations: Institute of Fisheries Sciences, Pukyong National University, Busan 46041, Republic of Korea
- Published online on: April 28, 2021 https://doi.org/10.3892/mmr.2021.12125
Copyright: © Lee
et al. This is an open access article distributed under the
terms of Creative
Commons Attribution License.
Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
The protein extracted from red algae Pyropia yezoensis has various biological activities, including anti‑inflammatory, anticancer, antioxidant, and antiobesity properties. However, the effects of P. yezoensis protein (PYCP) on tumor necrosis factor‑α (TNF‑α)‑induced muscle atrophy are unknown. Therefore, the present study investigated the protective effects and related mechanisms of PYCP against TNF‑α‑induced myotube atrophy in C2C12 myotubes. Treatment with TNF‑α (20 ng/ml) for 48 h significantly reduced myotube viability and diameter and increased intracellular reactive oxygen species levels; these effects were significantly reversed in a dose‑dependent manner following treatment with 25‑100 µg/ml PYCP. PYCP inhibited the expression of TNF receptor‑1 in TNF‑α‑induced myotubes. In addition, PYCP markedly downregulated the nuclear translocation of nuclear factor‑κB (NF‑κB) by inhibiting the phosphorylation of inhibitor of κB. Furthermore, PYCP treatment suppressed 20S proteasome activity, IL‑6 production, and the expression of the E3 ubiquitin ligases, atrogin‑1/muscle atrophy F‑box and muscle RING‑finger protein‑1. Finally, PYCP treatment increased the protein expression levels of myoblast determination protein 1 and myogenin in TNF‑α‑induced myotubes. The present findings indicate that PYCP may protect against TNF‑α‑induced myotube atrophy by inhibiting the proinflammatory NF‑κB pathway.