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Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling

  • Authors:
    • Fang Yan
    • Yanyan Hao
    • Xinji Gong
    • Hu Sun
    • Jianbing Ding
    • Jing Wang
  • View Affiliations / Copyright

    Affiliations: School of Public Health, Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830011, P.R. China, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Department of Respiratory Medicine, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830054, P.R. China, Department of Immunology, College of Basic Medicine, Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830011, P.R. China
    Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 502
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    Published online on: May 9, 2021
       https://doi.org/10.3892/mmr.2021.12141
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Abstract

Accumulating evidence suggests that pulmonary expression of a disintegrin and metalloproteinase‑33 (ADAM33) serves a key role in the pathogenesis of airway remodeling‑related diseases, including asthma. Airway vascular proliferation has been recognized as a key feature of airway remodeling. Our previous study showed that ADAM33 is constitutively expressed in airway vascular smooth muscle cells in patients with asthma, suggesting a potential role of ADAM33 in regulating airway vascular remodeling. Using in vitro human aortic smooth muscle cells (HASMCs) and lentiviral vector carrying short hairpin RNA for ADAM33, the present study aimed to evaluate the influence of ADAM33 silencing on the proliferation and apoptosis of HASMCs and the underlying molecular pathways. Cellular proliferation was observed using the Cell Counting Kit‑8 method. Cellular apoptosis was evaluated with Annexin V‑PE/7‑AAD staining and flow cytometry. Reverse transcription‑quantitative PCR and western blotting were used to evaluate the changes in mRNA and protein levels of involved signaling molecules. It was found that silencing of ADAM33 expression in HASMCs significantly inhibited proliferation, but induced the apoptosis of HASMCs. These changes were accompanied by inhibition of the PI3K/AKT/ERK pathway and Bcl‑2, but an increase in Bax expression. These results suggested that constitutive expression of ADAM33 may be important to maintain a proliferative phenotype in HASMCs. The influences of ADAM33 on proliferation and apoptosis of HASMCs may involve regulation of PI3K/AKT/ERK and Bax/Bcl‑2 pathways. These findings suggested an important role of ADAM33 in airway vascular remodeling and potential therapeutic significance of ADAM33 inhibition in airway remodeling‑related diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Yan F, Hao Y, Gong X, Sun H, Ding J and Wang J: Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling. Mol Med Rep 24: 502, 2021.
APA
Yan, F., Hao, Y., Gong, X., Sun, H., Ding, J., & Wang, J. (2021). Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling. Molecular Medicine Reports, 24, 502. https://doi.org/10.3892/mmr.2021.12141
MLA
Yan, F., Hao, Y., Gong, X., Sun, H., Ding, J., Wang, J."Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling". Molecular Medicine Reports 24.1 (2021): 502.
Chicago
Yan, F., Hao, Y., Gong, X., Sun, H., Ding, J., Wang, J."Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling". Molecular Medicine Reports 24, no. 1 (2021): 502. https://doi.org/10.3892/mmr.2021.12141
Copy and paste a formatted citation
x
Spandidos Publications style
Yan F, Hao Y, Gong X, Sun H, Ding J and Wang J: Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling. Mol Med Rep 24: 502, 2021.
APA
Yan, F., Hao, Y., Gong, X., Sun, H., Ding, J., & Wang, J. (2021). Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling. Molecular Medicine Reports, 24, 502. https://doi.org/10.3892/mmr.2021.12141
MLA
Yan, F., Hao, Y., Gong, X., Sun, H., Ding, J., Wang, J."Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling". Molecular Medicine Reports 24.1 (2021): 502.
Chicago
Yan, F., Hao, Y., Gong, X., Sun, H., Ding, J., Wang, J."Silencing a disintegrin and metalloproteinase‑33 attenuates the proliferation of vascular smooth muscle cells via PI3K/AKT pathway: Implications in the pathogenesis of airway vascular remodeling". Molecular Medicine Reports 24, no. 1 (2021): 502. https://doi.org/10.3892/mmr.2021.12141
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