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lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR‑500a‑3p/XBP‑1 axis

  • Authors:
    • Yun Zhou
    • Zhenghong Sha
    • Yong Yang
    • Shuimei Wu
    • Hong Chen
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Zhongda Hospital Southeast University, Medical College of Southeast University, Nanjing, Jiangsu 210000, P.R. China, Department of General Surgery, Wuhu No. 1 People's Hospital, Wuhu, Anhui 241000, P.R. China, Department of Gastroenterology, Wuhu No. 1 People's Hospital, Wuhu, Anhui 241000, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 503
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    Published online on: May 9, 2021
       https://doi.org/10.3892/mmr.2021.12142
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Abstract

Gastric cancer is a serious malignant tumor. Despite progression in gastric cancer research in recent years, the specific molecular mechanism underlying the pathogenesis of the disease is not completely understood. Long non‑coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) affects the proliferation and metastasis of multiple types of tumor cells in colorectal cancer and breast cancer but its specific role in gastric cancer requires further investigation. The aim of the present study was to analyze the role of NEAT1 in gastric cancer. The expression of endoplasmic reticulum stress marker proteins and apoptosis‑related proteins in gastric cancer tissue and cell lines was analyzed using western blotting. The targeting relationship of NEAT1 and miR‑500a‑3p was analyzed using dual‑luciferase reporter assay. Cell proliferation was analyzed using CCK8 assay and colony formation assay while cell invasion was detected using Transwell assay. Cell apoptosis was analyzed using TUNEL staining and LC3 expression through immunofluorescent staining (IF). The results showed that lncRNA NEAT1‑overexpression gastric cancer cells were established to determine its effects on cell proliferation, invasion, apoptosis, autophagy and endoplasmic reticulum stress. Subsequently, microRNA (miR)‑500a was overexpressed in lncRNA NEAT1‑overexpression cells. Compared with the vector group, lncRNA NEAT1 overexpression significantly inhibited gastric cancer cell proliferation and invasion, but significantly promoted cell apoptosis. Furthermore, the results indicated that lncRNA NEAT1 targeted and downregulated the expression of miR‑500a‑3p, and miR‑500a‑3p targeted X‑box binding protein‑1 (XBP‑1) mRNA. lncRNA NEAT1 overexpression‑mediated inhibition of gastric cancer cell proliferation and invasion was significantly reversed by miR‑500a‑3p overexpression. Furthermore, compared with the vector group, the expression levels of endoplasmic reticulum stress‑related proteins (XBP‑1S/XBP‑1U ratio and 78‑kDa glucose‑regulated protein) and apoptosis‑related proteins (Bax and cleaved‑caspase‑3) were significantly upregulated by lncRNA NEAT1 overexpression; however, miR‑500a‑3p overexpression reversed lncRNA NEAT1 overexpression‑mediated effects on protein expression. The present study demonstrated that lncRNA NEAT1 inhibited gastric cancer cell proliferation and invasion, and promoted apoptosis by regulating the miR‑500a‑3p/XBP‑1 axis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou Y, Sha Z, Yang Y, Wu S and Chen H: lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR‑500a‑3p/XBP‑1 axis. Mol Med Rep 24: 503, 2021.
APA
Zhou, Y., Sha, Z., Yang, Y., Wu, S., & Chen, H. (2021). lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR‑500a‑3p/XBP‑1 axis. Molecular Medicine Reports, 24, 503. https://doi.org/10.3892/mmr.2021.12142
MLA
Zhou, Y., Sha, Z., Yang, Y., Wu, S., Chen, H."lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR‑500a‑3p/XBP‑1 axis". Molecular Medicine Reports 24.1 (2021): 503.
Chicago
Zhou, Y., Sha, Z., Yang, Y., Wu, S., Chen, H."lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR‑500a‑3p/XBP‑1 axis". Molecular Medicine Reports 24, no. 1 (2021): 503. https://doi.org/10.3892/mmr.2021.12142
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Y, Sha Z, Yang Y, Wu S and Chen H: lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR‑500a‑3p/XBP‑1 axis. Mol Med Rep 24: 503, 2021.
APA
Zhou, Y., Sha, Z., Yang, Y., Wu, S., & Chen, H. (2021). lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR‑500a‑3p/XBP‑1 axis. Molecular Medicine Reports, 24, 503. https://doi.org/10.3892/mmr.2021.12142
MLA
Zhou, Y., Sha, Z., Yang, Y., Wu, S., Chen, H."lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR‑500a‑3p/XBP‑1 axis". Molecular Medicine Reports 24.1 (2021): 503.
Chicago
Zhou, Y., Sha, Z., Yang, Y., Wu, S., Chen, H."lncRNA NEAT1 regulates gastric carcinoma cell proliferation, invasion and apoptosis via the miR‑500a‑3p/XBP‑1 axis". Molecular Medicine Reports 24, no. 1 (2021): 503. https://doi.org/10.3892/mmr.2021.12142
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