3,4,5‑Trihydroxycinnamic acid exerts anti‑inflammatory effects on TNF‑α/IFN‑γ‑stimulated HaCaT cells

Park,Ji-Won; Oh,Jae-Hoon; Hwang,Daseul; Kim,Seong-Man; Min,Jae-Hong; Seo,Ji-Yun; Chun,Wanjoo; Lee,Hee Jae; Oh,Sei-Ryang; Lee,Jae-Won; Ahn,Kyung-Seop

doi:10.3892/mmr.2021.12148

3,4,5‑Trihydroxycinnamic acid exerts anti‑inflammatory effects on TNF‑α/IFN‑γ‑stimulated HaCaT cells

Authors:
- Ji-Won Park
- Jae-Hoon Oh
- Daseul Hwang
- Seong-Man Kim
- Jae-Hong Min
- Ji-Yun Seo
- Wanjoo Chun
- Hee Jae Lee
- Sei-Ryang Oh
- Jae-Won Lee
- Kyung-Seop Ahn
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Affiliations: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Chungcheongbuk‑do 28116, Republic of Korea, Department of Pharmacology, College of Medicine, Kangwon National University, Chuncheon, Gangwon‑do 24341, Republic of Korea
Published online on: May 11, 2021 https://doi.org/10.3892/mmr.2021.12148
Article Number: 509
Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

3,4,5‑Trihydroxycinnamic acid (THCA) exhibits anti‑inflammatory activity in acute or chronic inflammatory disorders, such as acute lung injury and asthma. The present study investigated the anti‑inflammatory activity of THCA in a tumor necrosis factor‑α/interferon‑γ (TI) mixture‑stimulated human keratinocyte cell line. The results of ELISA and reverse transcription‑quantitative PCR revealed that THCA reduced the secretion and mRNA expression levels of interleukin (IL)‑6; IL‑8; thymus and activation‑regulated chemokine; macrophage‑derived chemokine; regulated upon activation, normal T cell expressed and secreted; and monocyte chemoattractant protein‑1 in TI mixture‑stimulated HaCaT cells. In addition, the results of western blot analysis demonstrated that THCA exerted inhibitory activity on the activation of AKT, ERK and nuclear factor‑κB in TI mixture‑stimulated HaCaT cells. Furthermore, THCA upregulated the expression levels of heme oxygenase‑1 and NAD(P)H:quinone oxidoreductase 1, and the activation of nuclear factor erythroid 2‑related factor 2 in HaCaT cells. These results demonstrated that THCA may exhibit anti‑inflammatory activity in activated HaCaT cells.

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