Open Access

3,4,5‑Trihydroxycinnamic acid exerts anti‑inflammatory effects on TNF‑α/IFN‑γ‑stimulated HaCaT cells

  • Authors:
    • Ji-Won Park
    • Jae-Hoon Oh
    • Daseul Hwang
    • Seong-Man Kim
    • Jae-Hong Min
    • Ji-Yun Seo
    • Wanjoo Chun
    • Hee Jae Lee
    • Sei-Ryang Oh
    • Jae-Won Lee
    • Kyung-Seop Ahn
  • View Affiliations

  • Published online on: May 11, 2021     https://doi.org/10.3892/mmr.2021.12148
  • Article Number: 509
  • Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

3,4,5‑Trihydroxycinnamic acid (THCA) exhibits anti‑inflammatory activity in acute or chronic inflammatory disorders, such as acute lung injury and asthma. The present study investigated the anti‑inflammatory activity of THCA in a tumor necrosis factor‑α/interferon‑γ (TI) mixture‑stimulated human keratinocyte cell line. The results of ELISA and reverse transcription‑quantitative PCR revealed that THCA reduced the secretion and mRNA expression levels of interleukin (IL)‑6; IL‑8; thymus and activation‑regulated chemokine; macrophage‑derived chemokine; regulated upon activation, normal T cell expressed and secreted; and monocyte chemoattractant protein‑1 in TI mixture‑stimulated HaCaT cells. In addition, the results of western blot analysis demonstrated that THCA exerted inhibitory activity on the activation of AKT, ERK and nuclear factor‑κB in TI mixture‑stimulated HaCaT cells. Furthermore, THCA upregulated the expression levels of heme oxygenase‑1 and NAD(P)H:quinone oxidoreductase 1, and the activation of nuclear factor erythroid 2‑related factor 2 in HaCaT cells. These results demonstrated that THCA may exhibit anti‑inflammatory activity in activated HaCaT cells.
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July-2021
Volume 24 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Park J, Oh J, Hwang D, Kim S, Min J, Seo J, Chun W, Lee HJ, Oh S, Lee J, Lee J, et al: 3,4,5‑Trihydroxycinnamic acid exerts anti‑inflammatory effects on TNF‑α/IFN‑γ‑stimulated HaCaT cells. Mol Med Rep 24: 509, 2021.
APA
Park, J., Oh, J., Hwang, D., Kim, S., Min, J., Seo, J. ... Ahn, K. (2021). 3,4,5‑Trihydroxycinnamic acid exerts anti‑inflammatory effects on TNF‑α/IFN‑γ‑stimulated HaCaT cells. Molecular Medicine Reports, 24, 509. https://doi.org/10.3892/mmr.2021.12148
MLA
Park, J., Oh, J., Hwang, D., Kim, S., Min, J., Seo, J., Chun, W., Lee, H. J., Oh, S., Lee, J., Ahn, K."3,4,5‑Trihydroxycinnamic acid exerts anti‑inflammatory effects on TNF‑α/IFN‑γ‑stimulated HaCaT cells". Molecular Medicine Reports 24.1 (2021): 509.
Chicago
Park, J., Oh, J., Hwang, D., Kim, S., Min, J., Seo, J., Chun, W., Lee, H. J., Oh, S., Lee, J., Ahn, K."3,4,5‑Trihydroxycinnamic acid exerts anti‑inflammatory effects on TNF‑α/IFN‑γ‑stimulated HaCaT cells". Molecular Medicine Reports 24, no. 1 (2021): 509. https://doi.org/10.3892/mmr.2021.12148