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Anti‑inflammatory effect of Chrysanthemum zawadskii, peppermint, Glycyrrhiza glabra herbal mixture in lipopolysaccharide‑stimulated RAW264.7 macrophages

  • Authors:
    • Byoung Ok Cho
    • Jae Young Shin
    • Hyun Ju Kang
    • Ji Hyeon Park
    • Suping Hao
    • Feng Wang
    • Seon Il Jang
  • View Affiliations / Copyright

    Affiliations: Department of Food Science, Institute of Health Science, Jeonju University, Jeonju‑si, Jeollabuk‑do 55069, Republic of Korea, Department of Food Science and Technology, Jeonbuk National University, Jeonju‑si, Jeollabuk‑do 54896, Republic of Korea, Department of Health Management, Jeonju University, Jeonju‑si, Jeollabuk‑do 55069, Republic of Korea
    Copyright: © Cho et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 532
    |
    Published online on: May 25, 2021
       https://doi.org/10.3892/mmr.2021.12171
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Abstract

The normal inflammatory reaction protects the body from harmful external factors, whereas abnormal chronic inflammation can cause various diseases, including cancer. The purpose of the present study was to investigate the anti‑inflammatory activity of a mixture of Chrysanthemum zawadskii, peppermint and Glycyrrhiza glabra (CPG) by analyzing the expression levels of inflammatory mediators, cytokines and transcription factors in lipopolysaccharide (LPS)‑stimulated Raw264.7 cells. A nitric oxide assay, ELISA, western blotting and immunofluorescence staining were performed to investigate the anti‑inflammatory activity of the CPG mixture. Pretreatment of Raw264.7 cells with CPG inhibited the increase of inflammatory mediators (inducible nitric oxide synthase, cyclooxygenase‑2 and IFN‑β) induced by LPS. Additionally, it inhibited the production of pro‑inflammatory cytokines (TNF‑α, IL‑6 and IL‑1β). CPG suppressed LPS‑induced phosphorylation of STAT1, AKT, Iκb and NF‑κB. Furthermore, CPG inhibited the translocation of NF‑κB into the nucleus. In summary, CPG could inhibit LPS‑induced inflammation, which occurs primarily through the AKT/Iκb/NF‑κB signaling pathway in RAW264.7 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Cho BO, Shin JY, Kang HJ, Park JH, Hao S, Wang F and Jang SI: Anti‑inflammatory effect of <em>Chrysanthemum zawadskii</em>, peppermint, <em>Glycyrrhiza</em> glabra herbal mixture in lipopolysaccharide‑stimulated RAW264.7 macrophages. Mol Med Rep 24: 532, 2021.
APA
Cho, B.O., Shin, J.Y., Kang, H.J., Park, J.H., Hao, S., Wang, F., & Jang, S.I. (2021). Anti‑inflammatory effect of <em>Chrysanthemum zawadskii</em>, peppermint, <em>Glycyrrhiza</em> glabra herbal mixture in lipopolysaccharide‑stimulated RAW264.7 macrophages. Molecular Medicine Reports, 24, 532. https://doi.org/10.3892/mmr.2021.12171
MLA
Cho, B. O., Shin, J. Y., Kang, H. J., Park, J. H., Hao, S., Wang, F., Jang, S. I."Anti‑inflammatory effect of <em>Chrysanthemum zawadskii</em>, peppermint, <em>Glycyrrhiza</em> glabra herbal mixture in lipopolysaccharide‑stimulated RAW264.7 macrophages". Molecular Medicine Reports 24.1 (2021): 532.
Chicago
Cho, B. O., Shin, J. Y., Kang, H. J., Park, J. H., Hao, S., Wang, F., Jang, S. I."Anti‑inflammatory effect of <em>Chrysanthemum zawadskii</em>, peppermint, <em>Glycyrrhiza</em> glabra herbal mixture in lipopolysaccharide‑stimulated RAW264.7 macrophages". Molecular Medicine Reports 24, no. 1 (2021): 532. https://doi.org/10.3892/mmr.2021.12171
Copy and paste a formatted citation
x
Spandidos Publications style
Cho BO, Shin JY, Kang HJ, Park JH, Hao S, Wang F and Jang SI: Anti‑inflammatory effect of <em>Chrysanthemum zawadskii</em>, peppermint, <em>Glycyrrhiza</em> glabra herbal mixture in lipopolysaccharide‑stimulated RAW264.7 macrophages. Mol Med Rep 24: 532, 2021.
APA
Cho, B.O., Shin, J.Y., Kang, H.J., Park, J.H., Hao, S., Wang, F., & Jang, S.I. (2021). Anti‑inflammatory effect of <em>Chrysanthemum zawadskii</em>, peppermint, <em>Glycyrrhiza</em> glabra herbal mixture in lipopolysaccharide‑stimulated RAW264.7 macrophages. Molecular Medicine Reports, 24, 532. https://doi.org/10.3892/mmr.2021.12171
MLA
Cho, B. O., Shin, J. Y., Kang, H. J., Park, J. H., Hao, S., Wang, F., Jang, S. I."Anti‑inflammatory effect of <em>Chrysanthemum zawadskii</em>, peppermint, <em>Glycyrrhiza</em> glabra herbal mixture in lipopolysaccharide‑stimulated RAW264.7 macrophages". Molecular Medicine Reports 24.1 (2021): 532.
Chicago
Cho, B. O., Shin, J. Y., Kang, H. J., Park, J. H., Hao, S., Wang, F., Jang, S. I."Anti‑inflammatory effect of <em>Chrysanthemum zawadskii</em>, peppermint, <em>Glycyrrhiza</em> glabra herbal mixture in lipopolysaccharide‑stimulated RAW264.7 macrophages". Molecular Medicine Reports 24, no. 1 (2021): 532. https://doi.org/10.3892/mmr.2021.12171
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