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Article Open Access

CXCR3 alleviates renal ischemia‑reperfusion injury via increase of Tregs

  • Authors:
    • Wenjing Xian
    • Jiali Wu
    • Qingshu Li
    • Xunsong Du
    • Na Wang
    • Daiyu Chen
    • Wuxi Gao
    • Jun Cao
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Department of Anesthesiology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, Department of Pathology, Molecular and Cancer Research Center, Chongqing Medical University, Chongqing 400016, P.R. China
    Copyright: © Xian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 541
    |
    Published online on: May 28, 2021
       https://doi.org/10.3892/mmr.2021.12180
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Abstract

Increasing evidence has demonstrated that regulatory T cells (Tregs) suppress innate immunity, as well as protect the kidneys from ischemia‑reperfusion injury (IRI) and offer a potentially effective strategy to prevent or alleviate renal IRI. The present study explored whether C‑X‑C motif chemokine receptor 3 (CXCR3) alleviated renal IRI by increasing Tregs. Male C57BL/6J mice were divided into sham‑surgery, IRI, CXCR3 overexpression (OE‑CXCR3)+IRI, PC61+IRI and OE‑CXCR3+PC61+IRI groups. Histopathological examination of the kidney was carried out using hematoxylin‑eosin and Masson staining. The levels of serum creatinine (Scr) and blood urea nitrogen (BUN) were measured. Blood and kidney levels of IL‑6, TNF‑α, C‑C motif chemokine ligand (CCL)‑2 and IL‑10 were detected by ELISA and western blotting. The levels of superoxide dismutase (SOD), glutathione peroxidase (GSH‑Px) and malondialdehyde (MDA) in kidney tissues were also measured to assess oxidative stress. The population of Tregs in the kidney was assessed using flow cytometry. The results demonstrated that administration of OE‑CXCR3 to IRI mice significantly decreased the levels of Scr, BUN, IL‑6, TNF‑α, CCL‑2 and MDA, increased the levels of IL‑10, SOD and GSH‑Px, and mitigated the morphologic injury and fibrosis induced by IR compared with the IRI group. In addition, administration of OE‑CXCR3 induced significant reductions in the expression levels of fibrosis‑related markers, including fibronectin and type IV collagen, and increased the number of Tregs. These roles of OE‑CXCR3 were significantly neutralized following deletion of Tregs with PC61 (anti‑CD25 antibody). Together, the present study demonstrated that injection of OE‑CXCR3 lentiviral vectors into animal models can alleviate renal IRI by increasing the number of Tregs. The results may be a promising approach for the treatment of renal IRI.
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1 

Smith SF, Hosgood SA and Nicholson ML: Ischemia-reperfusion injury in renal transplantation: 3 key signaling pathways in tubular epithelial cells. Kidney Int. 95:50–56. 2019. View Article : Google Scholar : PubMed/NCBI

2 

Hosszu A, Fekete A and Szabo AJ: Sex differences in renal ischemia-reperfusion injury. Am J Physiol Renal Physiol. 319:F149–F154. 2020. View Article : Google Scholar : PubMed/NCBI

3 

Kim M, Park SW, Kim M, Chen SW, Gerthoffer WT, D'Agati VD and Lee HT: Selective renal overexpression of human heat shock protein 27 reduces renal ischemia-reperfusion injury in mice. Am J Physiol Renal Physiol. 299:F347–F358. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Jang HR and Rabb H: Immune cells in experimental acute kidney injury. Nat Rev Nephrol. 11:88–101. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Fontenot JD and Rudensky AY: A well adapted regulatory contrivance: Regulatory T cell development and the forkhead family transcription factor Foxp3. Nat Immunol. 6:331–337. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Luan H, Wang C, Sun J, Zhao L, Li L, Zhou B, Shao S, Shen X and Xu Y: Resolvin D1 protects against ischemia/reperfusion-induced acute kidney injury by increasing treg percentages via the ALX/FPR2 pathway. Front Physiol. 11:2852020. View Article : Google Scholar : PubMed/NCBI

7 

Haas J, Schwarz A, Korporal-Kuhnke M, Jarius S and Wildemann B: Myeloid dendritic cells exhibit defects in activation and function in patients with multiple sclerosis. J Neuroimmunol. 301:53–60. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Yazdani M, Khosropanah S, Hosseini A and Doroudchi M: Resting and activated natural tregs decrease in the peripheral blood of patients with atherosclerosis. Iran J Immunol. 13:249–262. 2016.PubMed/NCBI

9 

Ahmad SF, Zoheir KMA, Ansari MA, Nadeem A, Bakheet SA, Al-Ayadhi LY, Alzahrani MZ, Al-Shabanah OA, Al-Harbi MM and Attia SM: Dysregulation of Th1, Th2, Th17, and T regulatory cell-related transcription factor signaling in children with autism. Mol Neurobiol. 54:4390–4400. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Alroqi FJ and Chatila TA: T regulatory cell biology in health and disease. Curr Allergy Asthma Rep. 16:272016. View Article : Google Scholar : PubMed/NCBI

11 

Bai M, Zhang L, Fu B, Bai J, Zhang Y, Cai G, Bai X, Feng Z, Sun S and Chen X: IL-17A improves the efficacy of mesenchymal stem cells in ischemic-reperfusion renal injury by increasing Treg percentages by the COX-2/PGE2 pathway. Kidney Int. 93:814–825. 2018. View Article : Google Scholar : PubMed/NCBI

12 

Gandolfo MT, Jang HR, Bagnasco SM, Ko GJ, Agreda P, Satpute SR, Crow MT, King LS and Rabb H: Foxp3+ regulatory T cells participate in repair of ischemic acute kidney injury. Kidney Int. 76:717–729. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Kinsey GR, Huang L, Vergis AL, Li L and Okusa MD: Regulatory T cells contribute to the protective effect of ischemic preconditioning in the kidney. Kidney Int. 77:771–780. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Qin S, Rottman JB, Myers P, Kassam N, Weinblatt M, Loetscher M, Koch AE, Moser B and Mackay CR: The chemokine receptors CXCR3 and CCR5 mark subsets of T cells associated with certain inflammatory reactions. J Clin Invest. 101:746–754. 1998. View Article : Google Scholar : PubMed/NCBI

15 

Hasegawa H, Inoue A, Kohno M, Lei J, Miyazaki T, Yoshie O, Nose M and Yasukawa M: Therapeutic effect of CXCR3-expressing regulatory T cells on liver, lung and intestinal damages in a murine acute GVHD model. Gene Ther. 15:171–182. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, et al: Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J Immunol. 184:2886–2898. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Jun C, Qingshu L, Ke W, Ping L, Jun D, Jie L and Su M: Protective effect of CXCR3(+)CD4(+)CD25(+)Foxp3(+) regulatory T cells in renal ischemia-reperfusion injury. Mediators Inflamm. 2015:3609732015. View Article : Google Scholar : PubMed/NCBI

18 

Gu J, Sun P, Zhao H, Watts HR, Sanders RD, Terrando N, Xia P, Maze M and Ma D: Dexmedetomidine provides renoprotection against ischemia-reperfusion injury in mice. Crit Care. 15:R1532011. View Article : Google Scholar : PubMed/NCBI

19 

Zhang Q, Luan H, Wang L, He F, Zhou H, Xu X, Li X, Xu Q, Niki T, Hirashima M, et al: Galectin-9 ameliorates anti-GBM glomerulonephritis by inhibiting Th1 and Th17 immune responses in mice. Am J Physiol Renal Physiol. 306:F822–F832. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Fiorina P, Ansari MJ, Jurewicz M, Barry M, Ricchiuti V, Smith RN, Shea S, Means TK, Auchincloss H Jr, Luster AD, et al: Role of CXC chemokine receptor 3 pathway in renal ischemic injury. J Am Soc Nephrol. 17:716–723. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Zhang C, Zheng L, Li L, Wang L, Li L, Huang S, Gu C, Zhang L, Yang C, Zhu T and Rong R: Rapamycin protects kidney against ischemia reperfusion injury through recruitment of NKT cells. J Transl Med. 12:2242014. View Article : Google Scholar : PubMed/NCBI

23 

Ichimura T, Bonventre JV, Bailly V, Wei H, Hession CA, Cate RL and Sanicola M: Kidney injury molecule-1 (KIM-1), a putative epithelial cell adhesion molecule containing a novel immunoglobulin domain, is up-regulated in renal cells after injury. J Biol Chem. 273:4135–4142. 1998. View Article : Google Scholar : PubMed/NCBI

24 

Villa L, Buono R, Ferrandi M, Molinari I, Benigni F, Bettiga A, Colciago G, Ikehata M, Messaggio E, Rastaldi MP, et al: Ouabain contributes to kidney damage in a rat model of renal ischemia-reperfusion injury. Int J Mol Sci. 17:17282016. View Article : Google Scholar : PubMed/NCBI

25 

Lacotte S, Brun S, Muller S and Dumortier H: CXCR3, inflammation, and autoimmune diseases. Ann N Y Acad Sci. 1173:310–317. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Zhang X, Han J, Man K, Li X, Du J, Chu ESH, Go MYY, Sung JJY and Yu J: CXC chemokine receptor 3 promotes steatohepatitis in mice through mediating inflammatory cytokines, macrophages and autophagy. J Hepatol. 64:160–170. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Rocha VZ, Folco EJ, Ozdemir C, Sheikine Y, Christen T, Sukhova GK, Tang EHC, Bittencourt MS, Santos RD, Luster AD, et al: CXCR3 controls T-cell accumulation in fat inflammation. Arterioscler Thromb Vasc Biol. 34:1374–1381. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Altara R, Mallat Z, Booz GW and Zouein FA: The CXCL10/CXCR3 axis and cardiac inflammation: Implications for immunotherapy to treat infectious and noninfectious diseases of the heart. J Immunol Res. 2016:43963682016. View Article : Google Scholar : PubMed/NCBI

29 

Abron JD, Singh NP, Murphy AE, Mishra MK, Price RL, Nagarkatti M, Nagarkatti PS and Singh UP: Differential role of CXCR3 in inflammation and colorectal cancer. Oncotarget. 9:17928–17936. 2018. View Article : Google Scholar : PubMed/NCBI

30 

Karin N, Wildbaum G and Thelen M: Biased signaling pathways via CXCR3 control the development and function of CD4+ T cell subsets. J Leukoc Biol. 99:857–862. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Karin N and Wildbaum G: The role of chemokines in adjusting the balance between CD4+ effector T cell subsets and FOXp3-negative regulatory T cells. Int Immunopharmacol. 28:829–835. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Zohar Y, Wildbaum G, Novak R, Salzman AL, Thelen M, Alon R, Barsheshet Y, Karp CL and Karin N: CXCL11-dependent induction of FOXP3-negative regulatory T cells suppresses autoimmune encephalomyelitis. J Clin Invest. 124:2009–2022. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Chung AC and Lan HY: Chemokines in renal injury. J Am Soc Nephrol. 22:802–809. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Groom JR and Luster AD: CXCR3 in T cell function. Exp Cell Res. 317:620–631. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Bakheet SA, Ansari MA, Nadeem A, Attia SM, Alhoshani AR, Gul G, Al-Qahtani QH, Albekairi NA, Ibrahim KE and Ahmad SF: CXCR3 antagonist AMG487 suppresses rheumatoid arthritis pathogenesis and progression by shifting the Th17/Treg cell balance. Cell Signal. 64:1093952019. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xian W, Wu J, Li Q, Du X, Wang N, Chen D, Gao W and Cao J: CXCR3 alleviates renal ischemia‑reperfusion injury via increase of Tregs. Mol Med Rep 24: 541, 2021.
APA
Xian, W., Wu, J., Li, Q., Du, X., Wang, N., Chen, D. ... Cao, J. (2021). CXCR3 alleviates renal ischemia‑reperfusion injury via increase of Tregs. Molecular Medicine Reports, 24, 541. https://doi.org/10.3892/mmr.2021.12180
MLA
Xian, W., Wu, J., Li, Q., Du, X., Wang, N., Chen, D., Gao, W., Cao, J."CXCR3 alleviates renal ischemia‑reperfusion injury via increase of Tregs". Molecular Medicine Reports 24.1 (2021): 541.
Chicago
Xian, W., Wu, J., Li, Q., Du, X., Wang, N., Chen, D., Gao, W., Cao, J."CXCR3 alleviates renal ischemia‑reperfusion injury via increase of Tregs". Molecular Medicine Reports 24, no. 1 (2021): 541. https://doi.org/10.3892/mmr.2021.12180
Copy and paste a formatted citation
x
Spandidos Publications style
Xian W, Wu J, Li Q, Du X, Wang N, Chen D, Gao W and Cao J: CXCR3 alleviates renal ischemia‑reperfusion injury via increase of Tregs. Mol Med Rep 24: 541, 2021.
APA
Xian, W., Wu, J., Li, Q., Du, X., Wang, N., Chen, D. ... Cao, J. (2021). CXCR3 alleviates renal ischemia‑reperfusion injury via increase of Tregs. Molecular Medicine Reports, 24, 541. https://doi.org/10.3892/mmr.2021.12180
MLA
Xian, W., Wu, J., Li, Q., Du, X., Wang, N., Chen, D., Gao, W., Cao, J."CXCR3 alleviates renal ischemia‑reperfusion injury via increase of Tregs". Molecular Medicine Reports 24.1 (2021): 541.
Chicago
Xian, W., Wu, J., Li, Q., Du, X., Wang, N., Chen, D., Gao, W., Cao, J."CXCR3 alleviates renal ischemia‑reperfusion injury via increase of Tregs". Molecular Medicine Reports 24, no. 1 (2021): 541. https://doi.org/10.3892/mmr.2021.12180
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