Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2021 Volume 24 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2021 Volume 24 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Long non‑coding RNA SNHG20 promotes cell proliferation, migration and invasion in retinoblastoma via the miR‑335‑5p/E2F3 axis

  • Authors:
    • Jing Song
    • Ziping Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu 222000, P.R. China
  • Article Number: 543
    |
    Published online on: May 29, 2021
       https://doi.org/10.3892/mmr.2021.12182
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Current therapies for retinoblastoma (RB) are unsatisfactory and there is an urgent need for the development of new treatment modalities. Small nucleolar RNA host gene 20 (SNHG20) has been reported to serve a key oncogenic role in the development of various types of cancer, but its role in RB tumorigenesis remains to be fully determined. The present study aimed to investigate the expression patterns and biological roles of SNHG20 in RB. The expression levels of SNHG20 were measured via reverse transcription‑quantitative PCR in RB tissues and cell lines. The impact of SNHG20 status on cell proliferation, survival, migration and invasion was determined using small interfering RNA and a range of established experimental assays. The SNHG20/microRNA (miR)‑335‑5p/E2F transcription factor 3 (E2F3) signaling axis was further investigated using a dual‑luciferase activity reporter system and an RNA pull‑down assay combined with bioinformatics analyses. SNHG20 expression was significantly increased in RB tissues and cell lines. Silencing of SNHG20 in RB cells was shown to inhibit cell proliferation, clonogenic survival, migration and invasion. Moreover, mechanistic investigations demonstrated that SNHG20 could enhance the expression of E2F3 by sponging of miR‑335‑5p. These data suggested that the long non‑coding RNA SNHG20 may promote cell proliferation, migration and invasion in RB via the miR‑335‑5p/E2F3 axis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Broaddus E, Topham A and Singh AD: Incidence of retinoblastoma in the USA: 1975–2004. Br J Ophthalmol. 93:21–23. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Zheng C, Schneider JW and Hsieh J: Role of RB1 in human embryonic stem cell-derived retinal organoids. Dev Biol. 462:197–207. 2020. View Article : Google Scholar : PubMed/NCBI

3 

Hu H, Zhang W, Wang Y, Huang D, Shi J, Li B, Zhang Y and Zhou Y: Characterization, treatment and prognosis of retinoblastoma with central nervous system metastasis. BMC Ophthalmol. 18:1072018. View Article : Google Scholar : PubMed/NCBI

4 

Berry JL, Kogachi K, Aziz HA, McGovern K, Zolfaghari E, Murphree AL, Jubran R and Kim JW: Risk of metastasis and orbital recurrence in advanced retinoblastoma eyes treated with systemic chemoreduction versus primary enucleation. Pediatr Blood Cancer. 64:102017. View Article : Google Scholar : PubMed/NCBI

5 

Chawla B and Singh R: Recent advances and challenges in the management of retinoblastoma. Indian J Ophthalmol. 65:133–139. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Perkel JM: Visiting ‘noncodarnia’. Biotechniques. 54:301, 303–304. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Fang Y and Fullwood MJ: Roles, functions, and mechanisms of long Non-coding RNAs in cancer. Genomics Proteomics Bioinformatics. 14:42–54. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Kopp F and Mendell JT: Functional classification and experimental dissection of long noncoding RNAs. Cell. 172:393–407. 2018. View Article : Google Scholar : PubMed/NCBI

9 

Zhao W, Ma X, Liu L, Chen Q, Liu Z, Zhang Z, Ma S, Wang Z, Li H, Wang Z and Wu J: SNHG20: A vital lncRNA in multiple human cancers. J Cell Physiol. 2019.(Epub ahead of print).

10 

Wang ZX, Zhao Y, Yu Y, Liu N, Zou QX, Liang FH, Cheng KP and Lin FW: Effects of lncRNA SNHG20 on proliferation and apoptosis of non-small cell lung cancer cells through Wnt/β-catenin signaling pathway. Eur Rev Med Pharmacol Sci. 24:230–237. 2020.PubMed/NCBI

11 

Wu X, Xiao Y, Zhou Y, Zhou Z and Yan W: lncRNA SNHG20 promotes prostate cancer migration and invasion via targeting the miR-6516-5p/SCGB2A1 axis. Am J Transl Res. 11:5162–5169. 2019.PubMed/NCBI

12 

Yu J, Shen J, Qiao X, Cao L, Yang Z, Ye H, Xi C, Zhou Q, Wang P and Gong Z: SNHG20/miR-140-5p/NDRG3 axis contributes to 5-fluorouracil resistance in gastric cancer. Oncol Lett. 18:1337–1343. 2019.PubMed/NCBI

13 

Liu J, Cheng LG and Li HG: LncRNA SNHG20 promoted the proliferation of glioma cells via sponging miR-4486 to regulate the MDM2-p53 pathway. Eur Rev Med Pharmacol Sci. 23:5323–5331. 2019.PubMed/NCBI

14 

Wang D, Dai J, Hou S and Qian Y: LncRNA SNHG20 predicts a poor prognosis and promotes cell progression in epithelial ovarian cancer. Biosci Rep. 39:BSR201821862019. View Article : Google Scholar : PubMed/NCBI

15 

Zeng J, Liu Z, Zhang C, Hong T, Zeng F, Guan J, Tang S and Hu Z: Prognostic value of long non-coding RNA SNHG20 in cancer: A meta-analysis. Medicine (Baltimore). 99:e192042020. View Article : Google Scholar : PubMed/NCBI

16 

Paraskevopoulou MD and Hatzigeorgiou AG: Analyzing MiRNA-LncRNA Interactions. Methods Mol Biol. 1402:271–286. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Huang YA, Huang ZA, You ZH, Zhu Z, Huang WZ, Guo JX and Yu CQ: Predicting lncRNA-miRNA interaction via graph convolution Auto-Encoder. Front Genet. 10:7582019. View Article : Google Scholar : PubMed/NCBI

18 

Du W, Tang H, Lei Z, Zhu J, Zeng Y, Liu Z and Huang JA: MiR-335-5p inhibits TGF-β1-induced epithelial-mesenchymal transition in non-small cell lung cancer via ROCK1. Respir Res. 20:2252019. View Article : Google Scholar : PubMed/NCBI

19 

Zhang D and Yang N: MiR-335-5p inhibits cell proliferation, migration and invasion in colorectal cancer through downregulating LDHB. J BUON. 24:1128–1136. 2019.PubMed/NCBI

20 

Luo L, Xia L, Zha B, Zuo C, Deng D, Chen M, Hu L, He Y, Dai F, Wu J, et al: miR-335-5p targeting ICAM-1 inhibits invasion and metastasis of thyroid cancer cells. Biomed Pharmacother. 106:983–990. 2018. View Article : Google Scholar : PubMed/NCBI

21 

Gao Y, Li H, Ma X, Fan Y, Ni D, Zhang Y, Huang Q, Liu K, Li X, Wang L, et al: E2F3 upregulation promotes tumor malignancy through the transcriptional activation of HIF-2α in clear cell renal cell carcinoma. Oncotarget. 8:54021–54036. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Feng Z, Peng C, Li D, Zhang D, Li X, Cui F, Chen Y and He Q: E2F3 promotes cancer growth and is overexpressed through copy number variation in human melanoma. Onco Targets Ther. 11:5303–5313. 2018. View Article : Google Scholar : PubMed/NCBI

23 

Zhao W, Wang S, Qin T and Wang W: Circular RNA (circ-0075804) promotes the proliferation of retinoblastoma via combining heterogeneous nuclear ribonucleoprotein K (HNRNPK) to improve the stability of E2F transcription factor 3 E2F3. J Cell Biochem. 121:3516–3525. 2020. View Article : Google Scholar : PubMed/NCBI

24 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Carlevaro-Fita J, Lanzos A, Feuerbach L, Hong C, Mas-Ponte D, Pedersen JS; PCAWG Drivers and Functional Interpretation Group, ; Johnson R; PCAWG Consortium, : Cancer LncRNA Census reveals evidence for deep functional conservation of long noncoding RNAs in tumorigenesis. Commun Biol. 3:562020. View Article : Google Scholar : PubMed/NCBI

26 

Plousiou M and Vannini I: Non-Coding RNAs in Retinoblastoma. Front Genet. 10:11552019. View Article : Google Scholar : PubMed/NCBI

27 

Yu Y, Gao R, Kaul Z, Li L, Kato Y, Zhang Z, Groden J, Kaul SC and Wadhwa R: Loss-of-function screening to identify miRNAs involved in senescence: Tumor suppressor activity of miRNA-335 and its new target CARF. Sci Rep. 6:301852016. View Article : Google Scholar : PubMed/NCBI

28 

Sandoval-Borquez A, Polakovicova I, Carrasco-Veliz N, Lobos-González L, Riquelme I, Carrasco-Avino G, Bizama C, Norero E, Owen GI, Roa JC and Corvalán AH: MicroRNA-335-5p is a potential suppressor of metastasis and invasion in gastric cancer. Clin Epigenetics. 9:1142017. View Article : Google Scholar : PubMed/NCBI

29 

Wang J, Wang X, Liu F and Fu Y: MicroRNA-335 inhibits colorectal cancer HCT116 cells growth and epithelial-mesenchymal transition (EMT) process by targeting Twist1. Pharmazie. 72:475–481. 2017.PubMed/NCBI

30 

Tang H, Zhu J, Du W, Liu S, Zeng Y, Ding Z, Zhang Y, Wang X, Liu Z and Huang J: CPNE1 is a target of miR-335-5p and plays an important role in the pathogenesis of non-small cell lung cancer. J Exp Clin Cancer Res. 37:1312018. View Article : Google Scholar : PubMed/NCBI

31 

Yao L, Li M, Hu J, Wang W and Gao M: miRNA-335-5p negatively regulates granulosa cell proliferation via SGK3 in PCOS. Reproduction. 156:439–449. 2018.PubMed/NCBI

32 

Cui N, Liu J, Xia H and Xu D: LncRNA SNHG20 contributes to cell proliferation and invasion by upregulating ZFX expression sponging miR-495-3p in gastric cancer. J Cell Biochem. 120:3114–3123. 2019. View Article : Google Scholar : PubMed/NCBI

33 

Lingling J, Xiangao J, Guiqing H, Jichan S, Feifei S and Haiyan Z: SNHG20 knockdown suppresses proliferation, migration and invasion, and promotes apoptosis in non-small cell lung cancer through acting as a miR-154 sponge. Biomed Pharmacother. 112:1086482019. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Song J and Zhang Z: Long non‑coding RNA SNHG20 promotes cell proliferation, migration and invasion in retinoblastoma via the miR‑335‑5p/E2F3 axis. Mol Med Rep 24: 543, 2021.
APA
Song, J., & Zhang, Z. (2021). Long non‑coding RNA SNHG20 promotes cell proliferation, migration and invasion in retinoblastoma via the miR‑335‑5p/E2F3 axis. Molecular Medicine Reports, 24, 543. https://doi.org/10.3892/mmr.2021.12182
MLA
Song, J., Zhang, Z."Long non‑coding RNA SNHG20 promotes cell proliferation, migration and invasion in retinoblastoma via the miR‑335‑5p/E2F3 axis". Molecular Medicine Reports 24.2 (2021): 543.
Chicago
Song, J., Zhang, Z."Long non‑coding RNA SNHG20 promotes cell proliferation, migration and invasion in retinoblastoma via the miR‑335‑5p/E2F3 axis". Molecular Medicine Reports 24, no. 2 (2021): 543. https://doi.org/10.3892/mmr.2021.12182
Copy and paste a formatted citation
x
Spandidos Publications style
Song J and Zhang Z: Long non‑coding RNA SNHG20 promotes cell proliferation, migration and invasion in retinoblastoma via the miR‑335‑5p/E2F3 axis. Mol Med Rep 24: 543, 2021.
APA
Song, J., & Zhang, Z. (2021). Long non‑coding RNA SNHG20 promotes cell proliferation, migration and invasion in retinoblastoma via the miR‑335‑5p/E2F3 axis. Molecular Medicine Reports, 24, 543. https://doi.org/10.3892/mmr.2021.12182
MLA
Song, J., Zhang, Z."Long non‑coding RNA SNHG20 promotes cell proliferation, migration and invasion in retinoblastoma via the miR‑335‑5p/E2F3 axis". Molecular Medicine Reports 24.2 (2021): 543.
Chicago
Song, J., Zhang, Z."Long non‑coding RNA SNHG20 promotes cell proliferation, migration and invasion in retinoblastoma via the miR‑335‑5p/E2F3 axis". Molecular Medicine Reports 24, no. 2 (2021): 543. https://doi.org/10.3892/mmr.2021.12182
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team