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HIGD‑1B inhibits hypoxia‑induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytes

  • Authors:
    • Yan Pang
    • Zhide Zhu
    • Zhihao Wen
    • Junshen Lu
    • Hao Lin
    • Meiling Tang
    • Zhiliang Xu
    • Jianqi Lu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The First Affiliated Hospital, Guangxi University of Chinese Medicine, Nanning, Guangxi 530023, P.R. China, Academic Affairs Section, Guangxi University of Chinese Medicine, Nanning, Guangxi 530000, P.R. China, Academic Affairs Section, Guangxi University of Traditional Chinese Medicine Attached Chinese Medicine School, Nanning, Guangxi 530001, P.R. China, Department of Geriatrics, Danzhou Traditional Chinese Medicine Hospital, Danzhou, Hainan 571700, P.R. China
    Copyright: © Pang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 549
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    Published online on: June 2, 2021
       https://doi.org/10.3892/mmr.2021.12188
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Abstract

The dynamic regulation of mitochondrial morphology is key for eukaryotic cells to manage physiological challenges. Therefore, it is important to understand the molecular basis of mitochondrial dynamic regulation. The aim of the present study was to explore the role of HIG1 hypoxia inducible domain family member 1B (HIGD‑1B) in hypoxia‑induced mitochondrial fragmentation. Protein expression was determined via western blotting. Immunofluorescence assays were performed to detect the subcellular location of HIGD‑1B. Cell Counting Kit‑8 assays and flow cytometry were carried out to measure cell viability and apoptosis, respectively. Protein interactions were evaluated by co‑immunoprecipitation. In the present study, it was found that HIGD‑1B serves a role in cell survival by maintaining the integrity of the mitochondria under hypoxic conditions. Knockdown of HIGD‑1B promoted mitochondrial fragmentation, while overexpression of HIGD‑1B increased survival by preventing activation of caspase‑3 and ‑9. HIGD‑1B expression was associated with cell viability and apoptosis in cardiomyocytes. Furthermore, HIGD‑1B delayed the cleavage process of optic atrophy 1 (OPA1) and stabilized mitochondrial morphology by interacting with OPA1. Collectively, the results from the present study identified a role for HIGD‑1B as an inhibitor of the mitochondrial fission in cardiomyocytes.
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Copy and paste a formatted citation
Spandidos Publications style
Pang Y, Zhu Z, Wen Z, Lu J, Lin H, Tang M, Xu Z and Lu J: HIGD‑1B inhibits hypoxia‑induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytes. Mol Med Rep 24: 549, 2021.
APA
Pang, Y., Zhu, Z., Wen, Z., Lu, J., Lin, H., Tang, M. ... Lu, J. (2021). HIGD‑1B inhibits hypoxia‑induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytes. Molecular Medicine Reports, 24, 549. https://doi.org/10.3892/mmr.2021.12188
MLA
Pang, Y., Zhu, Z., Wen, Z., Lu, J., Lin, H., Tang, M., Xu, Z., Lu, J."HIGD‑1B inhibits hypoxia‑induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytes". Molecular Medicine Reports 24.2 (2021): 549.
Chicago
Pang, Y., Zhu, Z., Wen, Z., Lu, J., Lin, H., Tang, M., Xu, Z., Lu, J."HIGD‑1B inhibits hypoxia‑induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytes". Molecular Medicine Reports 24, no. 2 (2021): 549. https://doi.org/10.3892/mmr.2021.12188
Copy and paste a formatted citation
x
Spandidos Publications style
Pang Y, Zhu Z, Wen Z, Lu J, Lin H, Tang M, Xu Z and Lu J: HIGD‑1B inhibits hypoxia‑induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytes. Mol Med Rep 24: 549, 2021.
APA
Pang, Y., Zhu, Z., Wen, Z., Lu, J., Lin, H., Tang, M. ... Lu, J. (2021). HIGD‑1B inhibits hypoxia‑induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytes. Molecular Medicine Reports, 24, 549. https://doi.org/10.3892/mmr.2021.12188
MLA
Pang, Y., Zhu, Z., Wen, Z., Lu, J., Lin, H., Tang, M., Xu, Z., Lu, J."HIGD‑1B inhibits hypoxia‑induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytes". Molecular Medicine Reports 24.2 (2021): 549.
Chicago
Pang, Y., Zhu, Z., Wen, Z., Lu, J., Lin, H., Tang, M., Xu, Z., Lu, J."HIGD‑1B inhibits hypoxia‑induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytes". Molecular Medicine Reports 24, no. 2 (2021): 549. https://doi.org/10.3892/mmr.2021.12188
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