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Ginkgolide J protects human synovial cells SW982 via suppression of p38‑dependent production of pro‑inflammatory mediators

  • Authors:
    • Yujie Zhao
    • Yuan Chen
    • Jiayi Wang
    • Xue Zhu
    • Ke Wang
    • Yue Li
    • Fanfan Zhou
  • View Affiliations / Copyright

    Affiliations: NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu 214063, P.R. China, Department of Rheumatology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China, Faculty of Pharmacy, The University of Sydney, Sydney, NSW 2006, Australia
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 555
    |
    Published online on: June 2, 2021
       https://doi.org/10.3892/mmr.2021.12194
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Abstract

Fibroblast‑like synoviocytes (FLS) in the synovial lining play a key role in the pathological process of rheumatoid arthritis (RA), which produce pro‑inflammatory mediators to perpetuate inflammation and proteases to contribute to cartilage destruction. Ginkgolide J (GJ) is a subclass of ginkgolides (GGs) that exhibits anti‑inflammatory activity. In the present study, the protective effect of GJ on lipopolysaccharide (LPS)‑treated human synovial cells SW982 and its related mechanisms were investigated using various methods, including ELISA, Griess assay, western blotting, immunofluorescence analysis and p38 kinase activity assay. The results revealed that GJ pretreatment significantly attenuated LPS‑induced excess production of pro‑inflammatory mediators in SW982 cells via suppression of tumor necrosis factor‑α/interleukin (IL)‑1β/IL‑18/NF‑κB/NLR family pyrin domain containing 3, prostaglandin E2/cyclooxygenase‑2 and inducible nitric oxide synthase/nitric oxide signaling. Mechanistic studies revealed that p38 activation contributed to the LPS‑induced inflammatory response, and GJ pretreatment dose‑dependently attenuated p38 activation, indicating that the suppressive effect of GJ was achieved by targeting p38 signaling. These findings may contribute to the prevention and treatment of RA.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Y, Chen Y, Wang J, Zhu X, Wang K, Li Y and Zhou F: Ginkgolide J protects human synovial cells SW982 via suppression of p38‑dependent production of pro‑inflammatory mediators. Mol Med Rep 24: 555, 2021.
APA
Zhao, Y., Chen, Y., Wang, J., Zhu, X., Wang, K., Li, Y., & Zhou, F. (2021). Ginkgolide J protects human synovial cells SW982 via suppression of p38‑dependent production of pro‑inflammatory mediators. Molecular Medicine Reports, 24, 555. https://doi.org/10.3892/mmr.2021.12194
MLA
Zhao, Y., Chen, Y., Wang, J., Zhu, X., Wang, K., Li, Y., Zhou, F."Ginkgolide J protects human synovial cells SW982 via suppression of p38‑dependent production of pro‑inflammatory mediators". Molecular Medicine Reports 24.2 (2021): 555.
Chicago
Zhao, Y., Chen, Y., Wang, J., Zhu, X., Wang, K., Li, Y., Zhou, F."Ginkgolide J protects human synovial cells SW982 via suppression of p38‑dependent production of pro‑inflammatory mediators". Molecular Medicine Reports 24, no. 2 (2021): 555. https://doi.org/10.3892/mmr.2021.12194
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Y, Chen Y, Wang J, Zhu X, Wang K, Li Y and Zhou F: Ginkgolide J protects human synovial cells SW982 via suppression of p38‑dependent production of pro‑inflammatory mediators. Mol Med Rep 24: 555, 2021.
APA
Zhao, Y., Chen, Y., Wang, J., Zhu, X., Wang, K., Li, Y., & Zhou, F. (2021). Ginkgolide J protects human synovial cells SW982 via suppression of p38‑dependent production of pro‑inflammatory mediators. Molecular Medicine Reports, 24, 555. https://doi.org/10.3892/mmr.2021.12194
MLA
Zhao, Y., Chen, Y., Wang, J., Zhu, X., Wang, K., Li, Y., Zhou, F."Ginkgolide J protects human synovial cells SW982 via suppression of p38‑dependent production of pro‑inflammatory mediators". Molecular Medicine Reports 24.2 (2021): 555.
Chicago
Zhao, Y., Chen, Y., Wang, J., Zhu, X., Wang, K., Li, Y., Zhou, F."Ginkgolide J protects human synovial cells SW982 via suppression of p38‑dependent production of pro‑inflammatory mediators". Molecular Medicine Reports 24, no. 2 (2021): 555. https://doi.org/10.3892/mmr.2021.12194
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