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Article Open Access

Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress

  • Authors:
    • Yiyan Qiu
    • Guozheng Zhu
    • Canjun Zeng
    • Song Yuan
    • Yuepeng Qian
    • Zelin Ye
    • Shanwen Zhao
    • Runguang Li
  • View Affiliations / Copyright

    Affiliations: Department of Spine Surgery, Section II, Center for Orthopedic Surgery, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 510610, P.R. China, Division of Orthopedics and Traumatology, Department of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Orthopedics, Orthopaedic Hospital of Guangdong Province, Guangzhou, Guangdong 510610, P.R. China, Department of Orthopedics, Linzhi People's Hospital, Linzhi, Tibet 860000, P.R. China
    Copyright: © Qiu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 561
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    Published online on: June 4, 2021
       https://doi.org/10.3892/mmr.2021.12200
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Abstract

Exercise intervention has become one of the most effective methods to prevent and treat osteoporosis, which is a common age‑related disease and seriously affects the health and quality of life of the elderly. However, the molecular mechanism remains to be elucidated. The present study demonstrated the exercise‑induced promotion of osteogenic differentiation and inhibition of adipogenic differentiation in femur and tibia by establishing an animal exercise model using a treadmill exercise system. MicroRNA (miRNA/miR) and long non‑coding (lnc)RNA sequencing analyses identified 16 upregulated and two downregulated miRNAs in the exercise group, as well as 44 upregulated lncRNAs and 39 downregulated lncRNAs in the exercise group. There was increased expression of miR‑9942 and miR‑7704 in both the femur and tibia and an upregulation of miR‑30d, miR‑5100 and miR‑1260 in the femur of animals from the exercise group. In addition, four of the five most downregulated lncRNAs, including lncRNA MSTRG.2625, lncRNA MSTRG.1557, lncRNA MSTRG.691 and lncRNA MSTRG.7497, were demonstrated to be suppressed in both the femur and tibia after treadmill exercise. The results of the present study provided a valuable resource for further exploring the molecular mechanisms underlying the regulation of osteoporosis by exercise.
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Copy and paste a formatted citation
Spandidos Publications style
Qiu Y, Zhu G, Zeng C, Yuan S, Qian Y, Ye Z, Zhao S and Li R: Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress. Mol Med Rep 24: 561, 2021.
APA
Qiu, Y., Zhu, G., Zeng, C., Yuan, S., Qian, Y., Ye, Z. ... Li, R. (2021). Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress. Molecular Medicine Reports, 24, 561. https://doi.org/10.3892/mmr.2021.12200
MLA
Qiu, Y., Zhu, G., Zeng, C., Yuan, S., Qian, Y., Ye, Z., Zhao, S., Li, R."Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress". Molecular Medicine Reports 24.2 (2021): 561.
Chicago
Qiu, Y., Zhu, G., Zeng, C., Yuan, S., Qian, Y., Ye, Z., Zhao, S., Li, R."Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress". Molecular Medicine Reports 24, no. 2 (2021): 561. https://doi.org/10.3892/mmr.2021.12200
Copy and paste a formatted citation
x
Spandidos Publications style
Qiu Y, Zhu G, Zeng C, Yuan S, Qian Y, Ye Z, Zhao S and Li R: Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress. Mol Med Rep 24: 561, 2021.
APA
Qiu, Y., Zhu, G., Zeng, C., Yuan, S., Qian, Y., Ye, Z. ... Li, R. (2021). Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress. Molecular Medicine Reports, 24, 561. https://doi.org/10.3892/mmr.2021.12200
MLA
Qiu, Y., Zhu, G., Zeng, C., Yuan, S., Qian, Y., Ye, Z., Zhao, S., Li, R."Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress". Molecular Medicine Reports 24.2 (2021): 561.
Chicago
Qiu, Y., Zhu, G., Zeng, C., Yuan, S., Qian, Y., Ye, Z., Zhao, S., Li, R."Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress". Molecular Medicine Reports 24, no. 2 (2021): 561. https://doi.org/10.3892/mmr.2021.12200
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