miR‑205‑3p promotes lung cancer progression by targeting APBB2

Xu,Ling-Bin; Xiong,Jie; Zhang,Ya-Hui; Dai,Yun; Ren,Xiao-Ping; Ren,Ya-Juan; Han,Dong; Wei,Sheng-Hong; Qi,Min

doi:10.3892/mmr.2021.12227

miR‑205‑3p promotes lung cancer progression by targeting APBB2

Authors:
- Ling-Bin Xu
- Jie Xiong
- Ya-Hui Zhang
- Yun Dai
- Xiao-Ping Ren
- Ya-Juan Ren
- Dong Han
- Sheng-Hong Wei
- Min Qi
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Affiliations: Department of Pulmonary and Critical Care Medicine No. 2, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China, Department of Orthopaedics, Xi'an Daxing Hospital, Xi'an, Shaanxi 710016, P.R. China, Department of Thoracic Surgery, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China, Imaging Center, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China
Published online on: June 16, 2021 https://doi.org/10.3892/mmr.2021.12227
Article Number: 588
Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Non‑small cell lung cancer (NSCLC), a leading cause of cancer‑associated mortality, has resulted in low survival rates and a high mortality worldwide. Accumulating evidence has suggested that microRNAs (miRs) play critical roles in the regulation of cancer progression and the present study aimed to explore the underlying mechanism of miR‑205 in NSCLC. Reverse transcription‑quantitative PCR was performed, which determined that miR‑205 expression was upregulated in NSCLC, and the present study detected the upregulation of miR‑205‑3p in a number of NSCLC cell lines and NSCLC tissues. In addition, the mediation of amyloid β precursor protein‑binding family B member 2 (APBB2) by miR‑205‑3p was demonstrated. Moreover, miR‑205‑3p was predicted to directly target the 3'untranslated region of APBB2, which was confirmed using a dual‑luciferase reporter assay. It was found that lentivirus mediated‑APBB2 knockdown could promote cellular viability and suppress apoptosis in NSCLC cells, as determined via MTT, TUNEL and flow cytometry assays. Thus, the current findings highlighted the potential promotive impact of miR‑205‑3p on NSCLC processes and may provide theoretical evidence for miR‑205‑3p as a potential clinical gene therapy target.

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