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Map of dimorphic switching‑related signaling pathways in Sporothrix schenckii based on its transcriptome

  • Authors:
    • Fangliang Zheng
    • Wei Gao
    • Ying Wang
    • Qingyan Chen
    • Qiuling Zhang
    • Xiuyan Jiang
    • Binbin Hou
    • Zhenying Zhang
  • View Affiliations / Copyright

    Affiliations: Academy of Life Science, Liaoning University, Shenyang, Liaoning 110036, P.R. China, Department of Dermatology, Shenzhen Shekou People's Hospital, Shenzhen, Guangdong 518067, P.R. China, Department of Dermatology, The Second Hospital of Dalian Medical University, Dalian, Liaoning 116021, P.R. China, Department of Dermatology, University of Hong Kong Shenzhen Hospital, Shenzhen, Guangdong 518000, P.R. China
    Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 646
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    Published online on: July 12, 2021
       https://doi.org/10.3892/mmr.2021.12285
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Abstract

Sporothrix schenckii (S. schenckii) induces sporotrichosis, which has gained attention in recent years due to its worldwide prevalence. The dimorphic switching process is essential for the pathogenesis of S. schenckii. Previously, overexpression of several signal transduction genes, including SsDRK1 and SsSte20, was observed during the mycelium‑to‑yeast transition; these were necessary for asexual development, yeast‑phase cell formation, cell wall integrity and melanin synthesis. However, the mechanisms of the signaling pathways during dimorphic switching of S. schenckii remain unclear. In the present study, transcriptome sequencing of the 48‑h induced yeast forms and mycelium of S. schenckii was performed. In total, 24,904,510 high‑quality clean reads were obtained from mycelium samples and 22,814,406 from 48‑h induced yeast form samples. Following assembly, 31,779 unigene sequences were obtained with 52.98% GC content (The proportion of guanine G and cytosine C to all bases in nucleic acid). The results demonstrated that 12,217 genes, including genes involved in signal transduction and chitin synthesis, were expressed differentially between the two stages. According to these results, a map of the signaling pathways, including two‑component and heterotrimeric G‑protein signaling systems, Ras and MAPK cascades associated with the dimorphic switch, was drawn. Taken together, the transcriptome data and analysis performed in the present study lay the foundation for further research into the molecular mechanisms controlling the dimorphic switch of S. schenckii and support the development of anti‑S. schenckii strategies targeting genes associated with signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Zheng F, Gao W, Wang Y, Chen Q, Zhang Q, Jiang X, Hou B and Zhang Z: Map of dimorphic switching‑related signaling pathways in <em>Sporothrix schenckii</em> based on its transcriptome. Mol Med Rep 24: 646, 2021.
APA
Zheng, F., Gao, W., Wang, Y., Chen, Q., Zhang, Q., Jiang, X. ... Zhang, Z. (2021). Map of dimorphic switching‑related signaling pathways in <em>Sporothrix schenckii</em> based on its transcriptome. Molecular Medicine Reports, 24, 646. https://doi.org/10.3892/mmr.2021.12285
MLA
Zheng, F., Gao, W., Wang, Y., Chen, Q., Zhang, Q., Jiang, X., Hou, B., Zhang, Z."Map of dimorphic switching‑related signaling pathways in <em>Sporothrix schenckii</em> based on its transcriptome". Molecular Medicine Reports 24.3 (2021): 646.
Chicago
Zheng, F., Gao, W., Wang, Y., Chen, Q., Zhang, Q., Jiang, X., Hou, B., Zhang, Z."Map of dimorphic switching‑related signaling pathways in <em>Sporothrix schenckii</em> based on its transcriptome". Molecular Medicine Reports 24, no. 3 (2021): 646. https://doi.org/10.3892/mmr.2021.12285
Copy and paste a formatted citation
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Spandidos Publications style
Zheng F, Gao W, Wang Y, Chen Q, Zhang Q, Jiang X, Hou B and Zhang Z: Map of dimorphic switching‑related signaling pathways in <em>Sporothrix schenckii</em> based on its transcriptome. Mol Med Rep 24: 646, 2021.
APA
Zheng, F., Gao, W., Wang, Y., Chen, Q., Zhang, Q., Jiang, X. ... Zhang, Z. (2021). Map of dimorphic switching‑related signaling pathways in <em>Sporothrix schenckii</em> based on its transcriptome. Molecular Medicine Reports, 24, 646. https://doi.org/10.3892/mmr.2021.12285
MLA
Zheng, F., Gao, W., Wang, Y., Chen, Q., Zhang, Q., Jiang, X., Hou, B., Zhang, Z."Map of dimorphic switching‑related signaling pathways in <em>Sporothrix schenckii</em> based on its transcriptome". Molecular Medicine Reports 24.3 (2021): 646.
Chicago
Zheng, F., Gao, W., Wang, Y., Chen, Q., Zhang, Q., Jiang, X., Hou, B., Zhang, Z."Map of dimorphic switching‑related signaling pathways in <em>Sporothrix schenckii</em> based on its transcriptome". Molecular Medicine Reports 24, no. 3 (2021): 646. https://doi.org/10.3892/mmr.2021.12285
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