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Article

Senescent RAW264.7 cells exhibit increased production of nitric oxide and release inducible nitric oxide synthase in exosomes

  • Authors:
    • Hirokazu Hattori
    • Kazuki Takaoka
    • Miho Ueta
    • Masayuki Oshitani
    • Joji Tamaoka
    • Kazuma Noguchi
    • Hiromitsu Kishimoto
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Hyogo College of Medicine, Nishinomiya, Hyogo 663‑8501, Japan
  • Article Number: 681
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    Published online on: July 27, 2021
       https://doi.org/10.3892/mmr.2021.12320
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Abstract

Aging cells not only cease growing, but also secrete various proteins such as inflammatory cytokines. This secretory phenomenon is known as the senescence‑associated secretory phenotype (SASP). The aim of the present study was to elucidate the effects of senescence on the differentiation of osteoclast precursors (OCPs) and corresponding SASP. RAW264.7 cells were used as OCPs and were cultured to passage (P)5, P10 and P20. Cell proliferation assays, senescence‑associated β‑galactosidase staining and telomere length quantification were subsequently performed, and it was revealed that replicative senescence was induced at P20. In addition, the level of tartrate‑resistant acid phosphatase activity in P20 cells treated with receptor activator of nuclear factor‑κB ligand was significantly lower than that in P5 and P10 cells. The SASP factors interleukin‑6, tumour necrosis factor‑α and nitric oxide were significantly increased in P20 culture supernatants compared with those in P5 and P10 supernatants. Furthermore, the number of exosomes at P20 was increased compared with that at P5 and P10, and inducible nitric oxide synthase (iNOS) was expressed in exosomes at P20, but not in exosomes at P5. In conclusion, the present study revealed that senescent RAW264.7 cells exhibit increased expression of SASP factors and release iNOS in exosomes.
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1 

López-Otín C, Blasco MA, Partridge L, Serrano M and Kroemer G: The hallmarks of aging. Cell. 153:1194–1217. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Singh T and Newman AB: Inflammatory markers in population studies of aging. Ageing Res Rev. 10:319–329. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Roberts S, Colombier P, Sowman A, Mennan C, Rolfing HD, Guicheux J and Edwards JR: Ageing in the musculoskeletal system: Cellular function and dysfunction throughout life. Acta Orthop. 87:15–25. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Marie PJ: Bone cell senescence: Mechanisms and perspectives. J Bone Miner Res. 29:1311–1321. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Katsimbri P: The biology of normal bone remodelling. Eur J Cancer Care (Engl):. 26:e127402017. View Article : Google Scholar : PubMed/NCBI

6 

Han BI, Hwang SH and Lee MA: Progressive reduction in autophagic capacity contributes to induction of replicative senescence in Hs68 cells. Int J Biochem Cell Biol. 92:18–25. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Boyce BF, Zuscik MJ and Xing L: Biology of bone and cartilage. Genetics of Bone Biology and Skeletal Disease. Thakker RV, Eisman J, Igarashi T and Whyte MP: Elsevier; London: pp. 3–24. 2012

8 

Cao JJ, Wronski TJ, Iwaniec U, Phleger L, Kurimoto P, Boudignon B and Halloran BP: Aging increases stromal/osteoblastic cell-induced osteoclastogenesis and alters the osteoclast precursor pool in the mouse. J Bone Miner Res. 20:1659–1668. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Ahmed AS, Sheng MH, Wasnik S, Baylink DJ and Lau KW: Effect of aging on stem cells. World J Exp Med. 7:1–10. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Coppe JP, Patil CK, Rodier F, Sun Y, Munoz DP, Goldstein J, Nelson PS, Desprez PY and Campisi J: Senescence-associated secretory phenotypes reveal cell-nonautonomous functions of oncogenic RAS and the p53 tumor suppressor. PLoS Biol. 6:2853–2868. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Kim HN, Chang J, Shao L, Han L, Iyer S, Manolagas SC, O'Brien CA, Jilka RL, Zhou D and Almeida M: DNA damage and senescence in osteoprogenitors expressing Osx1 may cause their decrease with age. Aging Cell. 16:693–703. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Farr JN, Fraser DG, Wang H, Jaehn K, Ogrodnik MB, Weivoda MM, Drake MT, Tchkonia T, LeBrasseur NK, Kirkland JL, et al: Identification of senescent cells in the bone microenvironment. J Bone Miner Res. 31:1920–1929. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Collin-Osdoby P and Osdoby P: RANKL-mediated osteoclast formation from murine RAW264.7 cells. Methods Mol Biol. 816:187–202. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Ueta M, Takaoka K, Yamamura M, Maeda H, Tamaoka J, Nakano Y, Nouchi K and Kishimoto H: Effects of TGF-β1 on the migration and morphology of RAW264.7 cells in vitro. Mol Med Rep. 20:4331–4339. 2019.PubMed/NCBI

15 

Wang Z, Gao J, Ohno Y, Liu H and Xu C: Rosiglitazone ameliorates senescence and promotes apoptosis in ovarian cancer induced by Olaparib. Cancer Chemother Pharmacol. 85:273–284. 2020. View Article : Google Scholar : PubMed/NCBI

16 

Nezu T, Hosomi N, Takahashi T, Anno K, Aoki S, Shimamoto A, Maruyama H, Hayashi T, Matsumoto M and Tahara H: Telomere G-tail length is a promising biomarker related to white matter lesions and endothelial dysfunction in patients with cardiovascular risk: A cross-sectional study. EBioMedicine. 30:960–967. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Takahashi N, Yamana H, Yoshiki S, Roodman GD, Mundy GR, Jones SJ, Boyde A and Suda T: Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures. Endocrinology. 122:1373–1382. 1988. View Article : Google Scholar : PubMed/NCBI

18 

Guevara I, Iwanejko J, Dembinska-Kiec A, Pankiewicz J, Wanat A, Anna P, Golabek I, Bartus S, Malczewska-Malec M and Szczudlik A: Determination of nitrite/nitrate in human biological material by the simple Griess reaction. Clin Chim Acta. 274:177–188. 1998. View Article : Google Scholar : PubMed/NCBI

19 

Kim JH, Lee CH and Lee SW: Exosomal transmission of microRNA from HCV replicating cells stimulates transdifferentiation in hepatic stellate cells. Mol Ther Nucleic Acids. 14:483–497. 2019. View Article : Google Scholar : PubMed/NCBI

20 

Li Y, Yang Y, Xiong A, Wu X, Xie J, Han S and Zhao S: Comparative gene expression analysis of lymphocytes treated with exosomes derived from ovarian cancer and ovarian cysts. Front Immunol. 8:6072017. View Article : Google Scholar : PubMed/NCBI

21 

Wermuth PJ, Piera-Velazquez S and Jimenez SA: Exosome isolated from serum of systemic sclerosis patients display alterations in their content of profibrotic and antifibrotic microRNA and induce a profibrotic phenotype in cultured normal dermal fibroblasts. Clin Exp Rheumatol. 35:21–30. 2017.PubMed/NCBI

22 

Hashitani S, Urade M, Nishimura N, Maeda T, Takaoka K, Noguchi K and Sakurai K: Apoptosis induction and enhancement of cytotoxicity of anticancer drugs by celecoxib, a selective cyclooxygenase-2 inhibitor, in human head and neck carcinoma cell lines. Int J Oncol. 23:665–672. 2003.PubMed/NCBI

23 

Simonet WS, Lacey DL, Dunstan CR, Kelley M, Chang MS, Luthy R, Nguyen HQ, Wooden S, Bennett L, Boone T, et al: Osteoprotegerin: A novel secreted protein involved in the regulation of bone density. Cell. 89:309–319. 1997. View Article : Google Scholar : PubMed/NCBI

24 

Harding CV, Heuser JE and Stahl PD: Exosomes: Looking back three decades and into the future. J Cell Biol. 200:367–371. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Raposo G and Stoorvogel W: Extracellular vesicles: Exosomes, microvesicles, and friends. J Cell Biol. 200:373–383. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Hayflick L and Moorhead PS: The serial cultivation of human diploid cell strains. Exp Cell Res. 25:585–621. 1961. View Article : Google Scholar : PubMed/NCBI

27 

Campisi J and d'Adda di Fagagna F: Cellular senescence: When bad things happen to good cells. Nat Rev Mol Cell Biol. 8:729–740. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Zeng X: Human embryonic stem cells: Mechanisms to escape replicative senescence? Stem Cell Rev. 3:270–279. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Delfarah A, Parrish S, Junge JA, Yang J, Seo F, Li S, Mac J, Wang P, Fraser SE and Graham NA: Inhibition of nucleotide synthesis promotes replicative senescence of human mammary epithelial cells. J Biol Chem. 294:10564–10578. 2019. View Article : Google Scholar : PubMed/NCBI

30 

Alique M, Bodega G, Giannarelli C, Carracedo J and Ramírez R: MicroRNA-126 regulates hypoxia-inducible factor-1α which inhibited migration, proliferation, and angiogenesis in replicative endothelial senescence. Sci Rep. 9:73812019. View Article : Google Scholar : PubMed/NCBI

31 

Gan K, Xu L, Feng X, Zhang Q, Wang F, Zhang M and Tan W: Celastrol attenuates bone erosion in collagen-induced arthritis mice and inhibits osteoclast differentiation and function in RANKL-induced RAW264.7. Int Immunopharmacol. 24:239–246. 2015. View Article : Google Scholar : PubMed/NCBI

32 

GuezGuez A, Prod'Homme V, Mouska X, Baudot A, Blin-Wakkach C, Rottapel R and Deckert M: 3BPs adapter protein is required for receptor activator of NFκB ligand (RANKL)-induced osteoclast differentiation of RAW264.7. J Biol Chem. 285:20952–20963. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Itahana K, Campisi J and Dimri GP: Methods to detect biomarkers of cellular enescence: The senescence-associated beta-galactosidase assay. Methods Mol Biol. 371:21–31. 2007. View Article : Google Scholar : PubMed/NCBI

34 

Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, Medrano MM, Linskens M, Rubelj I, Pereira-Smith O, et al: A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci USA. 92:9363–9367. 1995. View Article : Google Scholar : PubMed/NCBI

35 

Debacq-Chainiaux F, Erusalimsky JD, Campisi J and Toussaint O: Protocols to detect senescence-associated beta-galactosidase (SA-betagal) activity, a biomarker of senescent cells in culture and in vivo. Nat Protoc. 4:1798–1806. 2009. View Article : Google Scholar : PubMed/NCBI

36 

Vogelstein B, Lane DP and Levine AJ: Surfing the p53 network. Nature. 408:307–310. 2000. View Article : Google Scholar : PubMed/NCBI

37 

Levine AJ and Oren M: The first 30 years of p53: Growing ever more complex. Nat Rev Cancer. 9:749–758. 2009. View Article : Google Scholar : PubMed/NCBI

38 

Vousden KH and Prives C: Blinded by the light: The Growing complexity of p53. Cell. 137:413–431. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Feng Z, Zhang H, Levine AJ and Jin S: The coordinate regulation of the p53 and mTOR pathways in cells. Proc Natl Acad Sci USA. 102:8204–8209. 2005. View Article : Google Scholar : PubMed/NCBI

40 

Budanov AV and Karin M: p53 target genes sestrin1 and sestrin2 connect genotoxic stress and mTOR signalling. Cell. 134:451–460. 2008. View Article : Google Scholar : PubMed/NCBI

41 

Weichhart T: mTOR as regulator of lifespan, aging, and cellular senescence: A mini-review. Gerontology. 64:127–134. 2018. View Article : Google Scholar : PubMed/NCBI

42 

Feng Z, Hu W, Teresky AK, Hernando E, Cordon-Cardo C and Levine AJ: Declining p53 function in the aging process: A possible mechanism for the increased tumor incidence in older populations. Proc Natl Acad Sci USA. 104:16633–16638. 2007. View Article : Google Scholar : PubMed/NCBI

43 

Campisi J: Aging, cellular senescence, and cancer. Annu Rev Physiol. 75:685–705. 2013. View Article : Google Scholar : PubMed/NCBI

44 

Soriani A, Zingoni A, Cerboni C, Lannitto ML, Ricciardi RR, Gialleonardo VD, Cippitelli M, Fionda C, Petrucci MT, Guarini A, et al: ATM-ATR-dependent up-regulation of DNAM-1 and NKG2D ligands on multiple myeloma cells by therapeutic agents results in enhanced NK-cell susceptibility and is associated with a senescent phenotype. Blood. 113:3503–3511. 2009. View Article : Google Scholar : PubMed/NCBI

45 

Coussens LM and Werb Z: Inflammation and cancer. Nature. 420:860–867. 2002. View Article : Google Scholar : PubMed/NCBI

46 

Takahashi S, Hakuta M, Aiba K, Ito Y, Horikoshi N, Miura M, Hatake K and Ogata E: Elevation of circulating plasma cytokines in cancer patients with high plasma parathyroid hormone-related protein levels. Endocr Relat Cancer. 10:403–407. 2003. View Article : Google Scholar : PubMed/NCBI

47 

Mabrouk N, Ghione S, Laurens V, Plenchette S, Bettaieb A and Paul C: Senescence and cancer: Role of nitric oxide (NO) in SASP. Cancers (Basel). 12:11452020. View Article : Google Scholar : PubMed/NCBI

48 

Campisi J: Senescent cells, tumor suppression, and organismal aging: Good citizens, bad neighbors. Cell. 120:513–522. 2015. View Article : Google Scholar : PubMed/NCBI

49 

Farr JN, Kaur J, Doolittle ML and Khosla S: Osteocyte cellular senescence. Curr Osteoporos Rep. 18:559–567. 2020. View Article : Google Scholar : PubMed/NCBI

50 

Walsh MC, Kim N, Kadono Y, Rho J, Lee SY, Lorenzo J and Choi Y: Osteoimmunology: Interplay between the immune system and bone metabolism. Annu Rev Immunol. 24:33–63. 2006. View Article : Google Scholar : PubMed/NCBI

51 

Azuma Y, Kaji K, Katogi R, Takeshita S and Kudo A: Tumor necrosis factor-alpha induces differentiation of and bone resorption by osteoclasts. J Biol Chem. 18:4858–4864. 2000. View Article : Google Scholar : PubMed/NCBI

52 

Komine M, Kukita A, Kukita T, Ogata Y, Hotokebuchi T and Kohashi O: Tumor necrosis factor-alpha cooperates with receptor activator of nuclear factor kappaB ligand in generation of osteoclasts in stromal cell-depleted rat bone marrow cell culture. Bone. 28:474–483. 2001. View Article : Google Scholar : PubMed/NCBI

53 

Palmqvist P, Persson E, Conaway HH and Lerner UH: IL-6, leukemia inhibitory factor, and oncostatin M stimulate bone resorption and regulate the expression of receptor activator of NF-kappa B ligand, osteoprotegerin, and receptor activator of NF-kappa B in mouse calvariae. J Immunol. 169:3353–3362. 2002. View Article : Google Scholar : PubMed/NCBI

54 

Feng W, Liu H, Luo T, Liu D, Du J, Sun J, Wang W, Han X, Yang K, Guo J, et al: Combination of IL-6 and sIL-6R differentially regulate varying levels of RANKL-induced osteoclastogenesis through NF-κB, ERK and JNK signaling pathways. Sci Rep. 7:414112017. View Article : Google Scholar : PubMed/NCBI

55 

Yokota K, Sato K, Miyazaki T, Kitaura H, Kayama H, Miyoshi F, Araki Y, Akiyama Y, Takeda K and Mimura T: Combination of tumor necrosis factor α and interleukin-6 induces mouse osteoclast-like cells with bone resorption activity both in vitro and in vivo. Arthritis Rheumatol. 66:121–129. 2014. View Article : Google Scholar : PubMed/NCBI

56 

MacIntyre I, Zaidi M, Alam AS, Datta HK, Moonga BS, Lidbury PS, Hecker M and Vane JR: Osteoclastic inhibition: An action of nitric oxide not mediated by cyclic GMP. Proc Natl Acad Sci USA. 88:2936–2940. 1991. View Article : Google Scholar : PubMed/NCBI

57 

Kasten TP, Collin-Osdoby P, Patel N, Osdoby P, Krukuwski M, Misko TP, Settle SL, Currie MG and Nickols GA: Potentiation of osteoclast bone-resorption activity by inhibition of nitric oxide synthase. Proc Natl Acad Sci USA. 91:3569–3573. 1994. View Article : Google Scholar : PubMed/NCBI

58 

Brandi M, Hukkanen M, Umeda T, Moradi-Bidhendi N, Bianchi S, Gross SS, Polak JM and Maclntyre I: Bidirectional regulation of osteoclast function by nitric oxide synthase isoforms. Proc Natl Acad Sci USA. 92:2954–2958. 1995. View Article : Google Scholar : PubMed/NCBI

59 

Nathan C and Xie QW: Nitric oxide synthases: Roles, tolls and controls. Cell. 78:915–918. 1994. View Article : Google Scholar : PubMed/NCBI

60 

Fukumura D, Kashiwagi S and Jain RK: The role of nitric oxide in tumor progression. Nat Rev Cancer. 6:521–534. 2006. View Article : Google Scholar : PubMed/NCBI

61 

Beckman JS, Beckman YW, Chen J, Marshall PA and Freeman BA: Apparent hydroxyl radical production by peroxynitrite: Implications for endothelial injury from nitric and superoxide. Proc Natl Acad Sci USA. 87:1620–1624. 1990. View Article : Google Scholar : PubMed/NCBI

62 

Zheng H, Yu X, Collin-Osdoby P and Osdoby P: RANKL stimulates inducible nitric-oxide synthase expression and nitric oxide production in developing osteoclasts. An autocrine negative feedback mechanism triggered by RANKL-induced interferon-beta via NF-kappaB that restrains osteoclastogenesis and bone resorption. J Biol Chem. 281:15809–15820. 2006. View Article : Google Scholar : PubMed/NCBI

63 

Takeda N, O'Dea EL, Doedens A, Kim JW, Weidemann A, Stockmann C, Asagiri M, Simon MC, Hoffmann A and Johnson RS: Differential activation and antagonistic function of HIF-{alpha} isoforms in macrophages are essential for NO homeostasis. Genes Dev. 24:491–501. 2010. View Article : Google Scholar : PubMed/NCBI

64 

Wu C, Rankin EB, Castellini L, Alcudia JF, LaGory EL, Andersen R, Rhodes SD, Wilson TL, Mohammad KS, Castillo AB, et al: Oxygen-sensing PHDs regulate bone homeostasis through the modulation of osteoprotegerin. Genes Dev. 29:817–831. 2015. View Article : Google Scholar : PubMed/NCBI

65 

Schorey JS, Cheng Y, Singh PP and Smith VL: Exosomes and other extracellular vesicles in host-pathogen interactions. EMBO Rep. 16:24–43. 2015. View Article : Google Scholar : PubMed/NCBI

66 

Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ and Lotvall JO: Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol. 9:654–659. 2007. View Article : Google Scholar : PubMed/NCBI

67 

Tkach M and Théry C: Communication by extracellular vesicles: Where we are and where we need to go. Cell. 164:1226–1232. 2016. View Article : Google Scholar : PubMed/NCBI

68 

Lehmann BD, Paine MS, Brooks AM, McCubrey JA, Renegar RH, Wang R and Terrian DM: Senescence-associated exosome release from human prostate cancer cells. Cancer Res. 68:7864–7871. 2008. View Article : Google Scholar : PubMed/NCBI

69 

Yoshida M, Satoh A, Lin JB, Mills KF, Sasaki Y, Rensing N, Wong M, Apte RS and Imai S: Extracellular Vesicle-contained eNAMPT delays aging and extends lifespan in mice. Cell Metab. 30:329–342. 2019. View Article : Google Scholar : PubMed/NCBI

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Hattori H, Takaoka K, Ueta M, Oshitani M, Tamaoka J, Noguchi K and Kishimoto H: Senescent RAW264.7 cells exhibit increased production of nitric oxide and release inducible nitric oxide synthase in exosomes. Mol Med Rep 24: 681, 2021.
APA
Hattori, H., Takaoka, K., Ueta, M., Oshitani, M., Tamaoka, J., Noguchi, K., & Kishimoto, H. (2021). Senescent RAW264.7 cells exhibit increased production of nitric oxide and release inducible nitric oxide synthase in exosomes. Molecular Medicine Reports, 24, 681. https://doi.org/10.3892/mmr.2021.12320
MLA
Hattori, H., Takaoka, K., Ueta, M., Oshitani, M., Tamaoka, J., Noguchi, K., Kishimoto, H."Senescent RAW264.7 cells exhibit increased production of nitric oxide and release inducible nitric oxide synthase in exosomes". Molecular Medicine Reports 24.3 (2021): 681.
Chicago
Hattori, H., Takaoka, K., Ueta, M., Oshitani, M., Tamaoka, J., Noguchi, K., Kishimoto, H."Senescent RAW264.7 cells exhibit increased production of nitric oxide and release inducible nitric oxide synthase in exosomes". Molecular Medicine Reports 24, no. 3 (2021): 681. https://doi.org/10.3892/mmr.2021.12320
Copy and paste a formatted citation
x
Spandidos Publications style
Hattori H, Takaoka K, Ueta M, Oshitani M, Tamaoka J, Noguchi K and Kishimoto H: Senescent RAW264.7 cells exhibit increased production of nitric oxide and release inducible nitric oxide synthase in exosomes. Mol Med Rep 24: 681, 2021.
APA
Hattori, H., Takaoka, K., Ueta, M., Oshitani, M., Tamaoka, J., Noguchi, K., & Kishimoto, H. (2021). Senescent RAW264.7 cells exhibit increased production of nitric oxide and release inducible nitric oxide synthase in exosomes. Molecular Medicine Reports, 24, 681. https://doi.org/10.3892/mmr.2021.12320
MLA
Hattori, H., Takaoka, K., Ueta, M., Oshitani, M., Tamaoka, J., Noguchi, K., Kishimoto, H."Senescent RAW264.7 cells exhibit increased production of nitric oxide and release inducible nitric oxide synthase in exosomes". Molecular Medicine Reports 24.3 (2021): 681.
Chicago
Hattori, H., Takaoka, K., Ueta, M., Oshitani, M., Tamaoka, J., Noguchi, K., Kishimoto, H."Senescent RAW264.7 cells exhibit increased production of nitric oxide and release inducible nitric oxide synthase in exosomes". Molecular Medicine Reports 24, no. 3 (2021): 681. https://doi.org/10.3892/mmr.2021.12320
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