Prevention of streptozotocin‑induced Neuro‑2a cell death by C8‑B4 microglia transformed with repetitive low‑dose lipopolysaccharide

  • Authors:
    • Haruka Mizobuchi
    • Kazushi Yamamoto
    • Masashi Yamashita
    • Hiroyuki Inagawa
    • Chie Kohchi
    • Gen-Ichiro Soma
  • View Affiliations

  • Published online on: July 30, 2021     https://doi.org/10.3892/mmr.2021.12328
  • Article Number: 687
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Abstract

Diabetes‑associated neuronal dysfunction (DAND) is one of the serious complications of diabetes, but there is currently no remedy for it. Streptozotocin [2‑deoxy‑2‑(3‑methy1‑3‑nitrosoureido) D‑glucopyranose; STZ] is one of the most well‑established diabetes inducers and has been used in vivo and in vitro DAND models. The aim of the present study was to demonstrate that C8‑B4 microglia transformed by the stimulus of repetitive low‑dose lipopolysaccharide (LPSx3‑microglia) prevent STZ‑induced Neuro‑2a neuronal cell death in vitro. The ELISA results showed that neurotrophin‑4/5 (NT‑4/5) secretion was promoted in LPSx3‑microglia and the cell viability assay with trypan blue staining revealed that the culture supernatant of LPSx3‑microglia prevented STZ‑induced neuronal cell death. In addition, reverse transcription‑quantitative PCR showed that neurons treated with the culture supernatant of LPSx3‑microglia promoted the gene expression of B‑cell lymphoma‑extra large and glucose‑dependent insulinotropic polypeptide receptor. Furthermore, the inhibition of tyrosine kinase receptor B, a receptor of NT‑4/5, suppressed the neuroprotective effect of LPSx3‑microglia. Taken together, the present study demonstrated that LPSx3‑microglia prevent STZ‑induced neuronal death and that NT‑4/5 may be involved in the neuroprotective mechanism of LPSx3‑microglia.
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October-2021
Volume 24 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Mizobuchi H, Yamamoto K, Yamashita M, Inagawa H, Kohchi C and Soma G: Prevention of streptozotocin‑induced Neuro‑2a cell death by C8‑B4 microglia transformed with repetitive low‑dose lipopolysaccharide. Mol Med Rep 24: 687, 2021
APA
Mizobuchi, H., Yamamoto, K., Yamashita, M., Inagawa, H., Kohchi, C., & Soma, G. (2021). Prevention of streptozotocin‑induced Neuro‑2a cell death by C8‑B4 microglia transformed with repetitive low‑dose lipopolysaccharide. Molecular Medicine Reports, 24, 687. https://doi.org/10.3892/mmr.2021.12328
MLA
Mizobuchi, H., Yamamoto, K., Yamashita, M., Inagawa, H., Kohchi, C., Soma, G."Prevention of streptozotocin‑induced Neuro‑2a cell death by C8‑B4 microglia transformed with repetitive low‑dose lipopolysaccharide". Molecular Medicine Reports 24.4 (2021): 687.
Chicago
Mizobuchi, H., Yamamoto, K., Yamashita, M., Inagawa, H., Kohchi, C., Soma, G."Prevention of streptozotocin‑induced Neuro‑2a cell death by C8‑B4 microglia transformed with repetitive low‑dose lipopolysaccharide". Molecular Medicine Reports 24, no. 4 (2021): 687. https://doi.org/10.3892/mmr.2021.12328