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Erastin‑induced ferroptosis causes physiological and pathological changes in healthy tissues of mice

  • Authors:
    • Jing Zhao
    • Ben Xu
    • Qingqing Xiong
    • Yunfei Feng
    • Huahua Du
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, College of Animal Science, Zhejiang University, Hangzhou, Zhejiang 310058, P.R. China, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, College of Animal Science, Zhejiang University, Hangzhou, Zhejiang 310058, P.R. China, Department of Endocrinology and Metabolism, The First Affiliated Hospital of Medical School of Zhejiang University, Hangzhou, Zhejiang 310000, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 713
    |
    Published online on: August 10, 2021
       https://doi.org/10.3892/mmr.2021.12352
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Abstract

Ferroptosis is a non‑apoptotic form of cell death that relies on iron and lipid peroxidation, which is associated with multiple pathological processes in several diseases. Erastin is a small molecule capable of initiating ferroptotic cell death in cancer cells, which has shown great potential for cancer therapy. However, the physiological and pathological role of erastin‑induced ferroptosis on healthy tissues has not been well characterized. The present study intraperitoneally injected erastin into healthy mice to detect the metabolic changes of several tissues of mice. Erastin injection induced typical characteristics of ferroptosis with higher level of serum iron and malondialdehyde and lower level of glutathione and glutathione peroxidase 4 protein. Erastin injection enhanced iron deposition in the brain, duodenum, kidney and spleen of mice. Erastin‑induced ferroptosis altered the blood index values, causing mild cerebral infarction of brain and enlarged glomerular volume of kidney. It also promoted the growth of duodenal epithelium with thicker, longer and denser villi in erastin‑treated mice. The findings provided evidence that erastin induced ferroptosis and caused pathological changes in healthy tissues of mice. This suggested that the anti‑tumor drug erastin was somewhat toxic to healthy tissues.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao J, Xu B, Xiong Q, Feng Y and Du H: Erastin‑induced ferroptosis causes physiological and pathological changes in healthy tissues of mice. Mol Med Rep 24: 713, 2021.
APA
Zhao, J., Xu, B., Xiong, Q., Feng, Y., & Du, H. (2021). Erastin‑induced ferroptosis causes physiological and pathological changes in healthy tissues of mice. Molecular Medicine Reports, 24, 713. https://doi.org/10.3892/mmr.2021.12352
MLA
Zhao, J., Xu, B., Xiong, Q., Feng, Y., Du, H."Erastin‑induced ferroptosis causes physiological and pathological changes in healthy tissues of mice". Molecular Medicine Reports 24.4 (2021): 713.
Chicago
Zhao, J., Xu, B., Xiong, Q., Feng, Y., Du, H."Erastin‑induced ferroptosis causes physiological and pathological changes in healthy tissues of mice". Molecular Medicine Reports 24, no. 4 (2021): 713. https://doi.org/10.3892/mmr.2021.12352
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao J, Xu B, Xiong Q, Feng Y and Du H: Erastin‑induced ferroptosis causes physiological and pathological changes in healthy tissues of mice. Mol Med Rep 24: 713, 2021.
APA
Zhao, J., Xu, B., Xiong, Q., Feng, Y., & Du, H. (2021). Erastin‑induced ferroptosis causes physiological and pathological changes in healthy tissues of mice. Molecular Medicine Reports, 24, 713. https://doi.org/10.3892/mmr.2021.12352
MLA
Zhao, J., Xu, B., Xiong, Q., Feng, Y., Du, H."Erastin‑induced ferroptosis causes physiological and pathological changes in healthy tissues of mice". Molecular Medicine Reports 24.4 (2021): 713.
Chicago
Zhao, J., Xu, B., Xiong, Q., Feng, Y., Du, H."Erastin‑induced ferroptosis causes physiological and pathological changes in healthy tissues of mice". Molecular Medicine Reports 24, no. 4 (2021): 713. https://doi.org/10.3892/mmr.2021.12352
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