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Article

Intermedin1‑47 inhibits high phosphate‑induced vascular smooth muscle cell calcification by regulating Wnt/β‑catenin signaling

  • Authors:
    • Yin Zhang
    • Naiwang Tang
    • Jinjie Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Geriatrics, Shanghai Fourth Rehabilitation Hospital, Shanghai 200042, P.R. China, Department of Respiratory, Central Hospital of Xuhui District, Shanghai 200031, P.R. China, Department of Cardiology, Central Hospital of Huangpu District, Shanghai 200002, P.R. China
  • Article Number: 733
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    Published online on: August 16, 2021
       https://doi.org/10.3892/mmr.2021.12373
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Abstract

Vascular calcification is a major risk factor for cardiovascular disease and accounts for a large proportion of deaths from cardiovascular disease in patients with chronic kidney disease. The high incidence, rapid progression and irreversibility of vascular smooth muscle cell (VSMC) calcification in patients has attracted attention. In the present study, the effect of intermedin1‑47 (IMD1‑47), an important isoform of intermedin, was investigated on the calcification of rat cardiovascular VSMCs induced by high phosphate (HP). To stimulate osteoblast‑like differentiation and calcification in rat VSMCs, 10 mM β‑sodium glycerophosphate was used. The VSMCs were then treated with three doses of IMD1‑47 and the effects of IMD1‑47 on VSMC calcification, on the expression of osteogenic markers [osteoprotegerin, Runt‑related transcription factor 2 (Runx2) and osteopontin (OPN)] and on alkaline phosphatase (ALP) activity were assessed. HP treatment significantly enhanced the cellular calcium content of VSMCs, the expression of osteogenic markers, and ALP activity, while IMD1‑47 significantly reversed these effects in a dose‑dependent manner. The protein expression levels of Wnt1, Wnt3a and active β‑catenin were determined and it was found that IMD1‑47 significantly inhibited their expression. Following β‑catenin silencing, the protein expression levels Runx2 and OPN were increased compared with the IMD1‑47 treatment alone, indicating a role for the Wnt/β‑catenin pathway in the effects of IMD1‑47 on osteogenic markers. The present study suggested that IMD1‑47 inhibited HP‑induced VSMC calcification by regulating the Wnt/β‑catenin signaling pathway.
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1 

Kendrick J and Chonchol M: The role of phosphorus in the development and progression of vascular calcification. Am J Kidney Dis. 58:826–834. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Pun PH, Smarz TR, Honeycutt EF, Shaw LK, Al-Khatib SM and Middleton JP: Chronic kidney disease is associated with increased risk of sudden cardiac death among patients with coronary artery disease. Kidney Int. 76:652–658. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Qunibi WY: Consequences of hyperphosphatemia in patients with end-stage renal disease (ESRD). Kidney Int Suppl. 90:S8–S12. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Moe SM and Chen NX: Pathophysiology of vascular calcification in chronic kidney disease. Circ Res. 95:560–567. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Ossareh S: Vascular calcification in chronic kidney disease: Mechanisms and clinical implications. Iran J Kidney Dis. 5:285–299. 2011.PubMed/NCBI

6 

Neven E, De Schutter TM, De Broe ME and D'Haese PC: Cell biological and physicochemical aspects of arterial calcification. Kidney Int. 79:1166–1177. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Persy V and D'Haese P: Vascular calcification and bone disease: The calcification paradox. Trends Mol Med. 15:405–416. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Gutierrez OM, Mannstadt M, Isakova T, Rauh-Hain JA, Tamez H, Shah A, Smith K, Lee H, Thadhani R, Jüppner H and Wolf M: Fibroblast growth factor 23 and mortality among patients undergoing hemodialysis. N Engl J Med. 359:584–592. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Lomashvili KA, Monier-Faugere MC, Wang X, Malluche HH and O'Neill WC: Effect of bisphosphonates on vascular calcification and bone metabolism in experimental renal failure. Kidney Int. 75:617–625. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Roh J, Chang CL, Bhalla A, Klein C and Hsu SY: Intermedin is a calcitonin/calcitonin gene-related peptide family peptide acting through the calcitonin receptor-like receptor/receptor activity-modifying protein receptor complexes. J Biol Chem. 279:7264–7274. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Takei Y, Inoue K, Ogoshi M, Kawahara T, Bannai H and Miyano S: Identification of novel adrenomedullin in mammals: A potent cardiovascular and renal regulator. FEBS Lett. 556:53–58. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Hong Y, Hay DL, Quirion R and Poyner DR: The pharmacology of adrenomedullin 2/intermedin. Br J Pharmacol. 166:110–120. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Zhao L, Peng DQ, Zhang J, Song JQ, Teng X, Yu YR, Tang CS and Qi YF: Extracellular signal-regulated kinase 1/2 activation is involved in intermedin1-53 attenuating myocardial oxidative stress injury induced by ischemia/reperfusion. Peptides. 33:329–335. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Chang JR, Duan XH, Zhang BH, Teng X, Zhou YB, Liu Y, Yu YR, Zhu Y, Tang CS and Qi YF: Intermedin1-53 attenuates vascular smooth muscle cell calcification by inhibiting endoplasmic reticulum stress via cyclic adenosine monophosphate/protein kinase A pathway. Exp Biol Med (Maywood). 238:1136–1146. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Chang JR, Guo J, Wang Y, Hou YL, Lu WW, Zhang JS, Yu YR, Xu MJ, Liu XY, Wang XJ, et al: Intermedin1-53 attenuates vascular calcification in rats with chronic kidney disease by upregulation of α-Klotho. Kidney Int. 89:586–600. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Grossini E, Molinari C, Mary DA, Uberti F, Caimmi PP and Vacca G: Intracoronary intermedin 1–47 augments cardiac perfusion and function in anesthetized pigs: Role of calcitonin receptors and beta-adrenoreceptor-mediated nitric oxide release. J Appl Physiol (1985). 107:1037–1050. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Golovina VA and Blaustein MP: Preparation of primary cultured mesenteric artery smooth muscle cells for fluorescent imaging and physiological studies. Nat Protoc. 1:2681–2687. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Willems BA, Furmanik M, Caron MM, Chatrou ML, Kusters DH, Welting TJ, Stock M, Rafael MS, Viegas CS, Simes DC, et al: Ucma/GRP inhibits phosphate-induced vascular smooth muscle cell calcification via SMAD-dependent BMP signalling. Sci Rep. 8:49612018. View Article : Google Scholar : PubMed/NCBI

19 

Wang J, Li J, Liu J, Xu M, Tong X and Wang J: Chlorogenic acid prevents isoproterenol-induced DNA damage in vascular smooth muscle cells. Mol Med Rep. 14:4063–4068. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Zhu D, Mackenzie NC, Millán JL, Farquharson C and MacRae VE: The appearance and modulation of osteocyte marker expression during calcification of vascular smooth muscle cells. PLoS One. 6:e195952011. View Article : Google Scholar : PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Hu H, Zhang W, Qiao Y, Jiang X, Liu X and Ding C: Antibacterial activity and increased bone marrow stem cell functions of Zn-incorporated TiO2 coatings on titanium. Acta Biomater. 8:904–915. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Leopold JA: Vascular calcification: Mechanisms of vascular smooth muscle cell calcification. Trends Cardiovasc Med. 25:267–274. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Moe SM, O'Neill KD, Duan D, Ahmed S, Chen NX, Leapman SB, Fineberg N and Kopecky K: Medial artery calcification in ESRD patients is associated with deposition of bone matrix proteins. Kidney Int. 61:638–647. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Wallin R, Wajih N, Greenwood GT and Sane DC: Arterial calcification: A review of mechanisms, animal models, and the prospects for therapy. Med Res Rev. 21:274–301. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Nakagawa Y, Ikeda K, Akakabe Y, Koide M, Uraoka M, Yutaka KT, Kurimoto-Nakano R, Takahashi T, Matoba S, Yamada H, et al: Paracrine osteogenic signals via bone morphogenetic protein-2 accelerate the atherosclerotic intimal calcification in vivo. Arterioscler Thromb Vasc Biol. 30:1908–1915. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Fakhry M, Roszkowska M, Briolay A, Bougault C, Guignandon A, Diaz-Hernandez JI, Diaz-Hernandez M, Pikula S, Buchet R, Hamade E, et al: TNAP stimulates vascular smooth muscle cell trans-differentiation into chondrocytes through calcium deposition and BMP-2 activation: Possible implication in atherosclerotic plaque stability. Biochim Biophys Acta Mol Basis Dis. 1863:643–653. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Giachelli CM: Vascular calcification mechanisms. J Am Soc Nephrol. 15:2959–2964. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Engelse MA, Neele JM, Bronckers AL, Pannekoek H and de Vries CJ: Vascular calcification: Expression patterns of the osteoblast-specific gene core binding factor alpha-1 and the protective factor matrix gla protein in human atherogenesis. Cardiovasc Res. 52:281–289. 2001. View Article : Google Scholar : PubMed/NCBI

30 

Sun Y, Byon CH, Yuan K, Chen J, Mao X, Heath JM, Javed A, Zhang K, Anderson PG and Chen Y: Smooth muscle cell-specific runx2 deficiency inhibits vascular calcification. Circ Res. 111:543–552. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Tyson KL, Reynolds JL, McNair R, Zhang Q, Weissberg PL and Shanahan CM: Osteo/chondrocytic transcription factors and their target genes exhibit distinct patterns of expression in human arterial calcification. Arterioscler Thromb Vasc Biol. 23:489–494. 2003. View Article : Google Scholar : PubMed/NCBI

32 

Chen Y, Hu Y, Yang L, Zhou J, Tang Y, Zheng L and Qin P: Runx2 alleviates high glucose-suppressed osteogenic differentiation via PI3K/AKT/GSK3β/β-catenin pathway. Cell Biol Int. 41:822–832. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Endres M, Hutmacher DW, Salgado AJ, Kaps C, Ringe J, Reis RL, Sittinger M, Brandwood A and Schantz JT: Osteogenic induction of human bone marrow-derived mesenchymal progenitor cells in novel synthetic polymer-hydrogel matrices. Tissue Eng. 9:689–702. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Hoshi K, Ejiri S and Ozawa H: Localizational alterations of calcium, phosphorus, and calcification-related organics such as proteoglycans and alkaline phosphatase during bone calcification. J Bone Miner Res. 16:289–298. 2001. View Article : Google Scholar : PubMed/NCBI

35 

Shioi A, Nishizawa Y, Jono S, Koyama H, Hosoi M and Morii H: Beta-glycerophosphate accelerates calcification in cultured bovine vascular smooth muscle cells. Arterioscler Thromb Vasc Biol. 15:2003–2009. 1995. View Article : Google Scholar : PubMed/NCBI

36 

Rong S, Zhao X, Jin X, Zhang Z, Chen L, Zhu Y and Yuan W: Vascular calcification in chronic kidney disease is induced by bone morphogenetic protein-2 via a mechanism involving the Wnt/β-catenin pathway. Cell Physiol Biochem. 34:2049–2060. 2014. View Article : Google Scholar : PubMed/NCBI

37 

Kramer I, Halleux C, Keller H, Pegurri M, Gooi JH, Weber PB, Feng JQ, Bonewald LF and Kneissel M: Osteocyte Wnt/beta-catenin signaling is required for normal bone homeostasis. Mol Cell Biol. 30:3071–3085. 2010. View Article : Google Scholar : PubMed/NCBI

38 

Meng J, Ma X, Wang N, Jia M, Bi L, Wang Y, Li M, Zhang H, Xue X, Hou Z, et al: Activation of GLP-1 receptor promotes bone marrow stromal cell osteogenic differentiation through β-catenin. Stem Cell Reports. 6:579–591. 2016. View Article : Google Scholar : PubMed/NCBI

39 

Xavier JR, Thakur T, Desai P, Jaiswal MK, Sears N, Cosgriff-Hernandez E, Kaunas R and Gaharwar AK: Bioactive nanoengineered hydrogels for bone tissue engineering: A growth-factor-free approach. ACS Nano. 9:3109–3118. 2015. View Article : Google Scholar : PubMed/NCBI

40 

Rajamannan NM, Subramaniam M, Caira F, Stock SR and Spelsberg TC: Atorvastatin inhibits hypercholesterolemia- induced calcification in the aortic valves via the Lrp5 receptor pathway. Circulation. 112:5243062005. View Article : Google Scholar : PubMed/NCBI

41 

Shao JS, Cheng SL, Pingsterhaus JM, Charlton-Kachigian N, Loewy AP and Towler DA: Msx2 promotes cardiovascular calcification by activating paracrine Wnt signals. J Clin Invest. 115:1210–1220. 2005. View Article : Google Scholar : PubMed/NCBI

42 

Cheng SL, Shao JS, Halstead LR, Distelhorst K, Sierra O and Towler DA: Activation of vascular smooth muscle parathyroid hormone receptor inhibits Wnt/beta-catenin signaling and aortic fibrosis in diabetic arteriosclerosis. Circ Res. 107:271–282. 2010. View Article : Google Scholar : PubMed/NCBI

43 

Al-Aly Z, Shao JS, Lai CF, Huang E, Cai J, Behrmann A, Cheng SL and Towler DA: Aortic Msx2-Wnt calcification cascade is regulated by TNF-alpha-dependent signals in diabetic Ldlr-/-mice. Arterioscler Thromb Vasc Biol. 27:2589–2596. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Tang N and Zhou J: Intermedin1‑47 inhibits high phosphate‑induced vascular smooth muscle cell calcification by regulating Wnt/β‑catenin signaling. Mol Med Rep 24: 733, 2021.
APA
Zhang, Y., Tang, N., & Zhou, J. (2021). Intermedin1‑47 inhibits high phosphate‑induced vascular smooth muscle cell calcification by regulating Wnt/β‑catenin signaling. Molecular Medicine Reports, 24, 733. https://doi.org/10.3892/mmr.2021.12373
MLA
Zhang, Y., Tang, N., Zhou, J."Intermedin1‑47 inhibits high phosphate‑induced vascular smooth muscle cell calcification by regulating Wnt/β‑catenin signaling". Molecular Medicine Reports 24.4 (2021): 733.
Chicago
Zhang, Y., Tang, N., Zhou, J."Intermedin1‑47 inhibits high phosphate‑induced vascular smooth muscle cell calcification by regulating Wnt/β‑catenin signaling". Molecular Medicine Reports 24, no. 4 (2021): 733. https://doi.org/10.3892/mmr.2021.12373
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Tang N and Zhou J: Intermedin1‑47 inhibits high phosphate‑induced vascular smooth muscle cell calcification by regulating Wnt/β‑catenin signaling. Mol Med Rep 24: 733, 2021.
APA
Zhang, Y., Tang, N., & Zhou, J. (2021). Intermedin1‑47 inhibits high phosphate‑induced vascular smooth muscle cell calcification by regulating Wnt/β‑catenin signaling. Molecular Medicine Reports, 24, 733. https://doi.org/10.3892/mmr.2021.12373
MLA
Zhang, Y., Tang, N., Zhou, J."Intermedin1‑47 inhibits high phosphate‑induced vascular smooth muscle cell calcification by regulating Wnt/β‑catenin signaling". Molecular Medicine Reports 24.4 (2021): 733.
Chicago
Zhang, Y., Tang, N., Zhou, J."Intermedin1‑47 inhibits high phosphate‑induced vascular smooth muscle cell calcification by regulating Wnt/β‑catenin signaling". Molecular Medicine Reports 24, no. 4 (2021): 733. https://doi.org/10.3892/mmr.2021.12373
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