Open Access

ADAM10 attenuates the development of abdominal aortic aneurysms in a mouse model

  • Authors:
    • Renfeng Qiu
    • Shuxiao Chen
    • Peixian Gao
    • Kun Luo
    • Xuedong Feng
    • Hai Yuan
    • Xuejun Wu
    • Gang Li
  • View Affiliations

  • Published online on: September 6, 2021     https://doi.org/10.3892/mmr.2021.12414
  • Article Number: 774
  • Copyright: © Qiu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

An abdominal aortic aneurysm (AAA) is a life‑threatening disease associated with a high mortality rate. At present, surgery or minimally invasive interventions are used in clinical treatment, especially for small aneurysms. However, the benefits of surgical repair are not obvious, and AAA ruptures can be prevented by aneurysm therapy to inhibit the growth of small aneurysms. Therefore, evaluating effective drugs to treat small AAAs is urgently required. Chronic inflammation is the main pathological feature of aneurysmal tissues. The aim of the present study was to investigate the protective role and underlying mechanism of ADAM metallopeptidase domain 10 (ADAM10). In the present study, a mouse model of AAA was established via porcine pancreatic elastase perfusion for 5 min per day for 14 days. ADAM10 (6 mg/kg) was injected intraperitoneally following 3 days of porcine pancreatic elastase perfusion in the ADAM10 group and the treatment continued for 10 days. The maximum inner luminal diameters of the infrarenal abdominal aortas were measured using an animal ultrasound system. The levels of high mobility group box 1 (HMGB1) and soluble receptor for advanced glycosylation end products in serum samples were measured by ELISA. Hematoxylin and eosin and elastin van Gieson staining were performed to observe morphology, integrity of the elastin layers and elastin degradation. CD68 expression was detected by immunohistochemical staining. Reverse transcription‑quantitative PCR and western blotting were used for detection of mRNA and protein levels. The gelatinolytic activities of MMP‑2 and MMP‑9 were quantified via gelatin zymography analysis. These results showed that ADAM10 inhibited HMGB1/RAGE/NF‑κB signaling and MMP activity in the pathogenesis of pancreatic elastase‑induced AAA, which provide insight into the molecular mechanism of AAA and suggested that ADAM10 may be a potential therapeutic target for AAA.
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November-2021
Volume 24 Issue 5

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Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Qiu R, Chen S, Gao P, Luo K, Feng X, Yuan H, Wu X and Li G: ADAM10 attenuates the development of abdominal aortic aneurysms in a mouse model. Mol Med Rep 24: 774, 2021
APA
Qiu, R., Chen, S., Gao, P., Luo, K., Feng, X., Yuan, H. ... Li, G. (2021). ADAM10 attenuates the development of abdominal aortic aneurysms in a mouse model. Molecular Medicine Reports, 24, 774. https://doi.org/10.3892/mmr.2021.12414
MLA
Qiu, R., Chen, S., Gao, P., Luo, K., Feng, X., Yuan, H., Wu, X., Li, G."ADAM10 attenuates the development of abdominal aortic aneurysms in a mouse model". Molecular Medicine Reports 24.5 (2021): 774.
Chicago
Qiu, R., Chen, S., Gao, P., Luo, K., Feng, X., Yuan, H., Wu, X., Li, G."ADAM10 attenuates the development of abdominal aortic aneurysms in a mouse model". Molecular Medicine Reports 24, no. 5 (2021): 774. https://doi.org/10.3892/mmr.2021.12414