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Long non‑coding RNA HHIP‑AS1 inhibits lung cancer epithelial‑mesenchymal transition and stemness by regulating PCDHGA9

  • Authors:
    • Zhuanzhuan Zhou
    • Yanping Lai
    • Shan Cao
    • Qifang Zhuo
    • Huiqin Tang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory and Critical Care Medicine, The Second Hospital of Tianjin Medical University, Tianjin 300211, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 845
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    Published online on: October 11, 2021
       https://doi.org/10.3892/mmr.2021.12485
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Abstract

The aim of the present study was to investigate the effect of hedgehog‑interacting protein antisense RNA 1 (HHIP‑AS1) on epithelial‑mesenchymal transition (EMT) and cellular stemness of human lung cancer cells by regulating the microRNA (miR)‑153‑3p/PCDHGA9 axis. Reverse transcription‑quantitative PCR was used to compare the expression of HHIP‑AS1 in lung cancer and adjacent normal lung tissues. In addition, the correlation of HHIP‑AS1 with E‑cadherin, Vimentin, N‑cadherin and Twist1 was analyzed. HHIP‑AS1 overexpression vector was transfected into lung cancer A549 and NCI‑H1299 cell lines. Cell Counting Kit‑8 and Transwell and clonogenic assays were used to detect the proliferation, invasion and clonogenesis of the lung cancer cells, respectively. The associations among HHIP‑AS1, miR‑153‑3p and PCDHGA9 were predicted by bioinformatics analysis and verified by a dual‑luciferase reporter system. The results showed that the expression of HHIP‑AS1 in lung cancer tissues was significantly lower than that in normal tissues (P<0.001). HHIP‑AS1 was positively correlated with E‑cadherin and negatively correlated with Vimentin, N‑cadherin and Twist1. HHIP‑AS1 overexpression inhibited the proliferation, invasion and clonal formation of the A549 and NCI‑H1299 cells. The luciferase reporter system verified that HHIP‑AS1 could adsorb miR‑153‑3p and that PCDHGA9 was the target gene of miR‑153‑3p. A549 cells were transfected with HHIP‑AS1 overexpression vector and miR‑153‑3p mimic, and the miR‑153‑3p mimic had a mitigating effect on HHIP‑AS1 inhibition (P<0.001). In conclusion, HHIP‑AS1 inhibits the EMT and stemness of lung cancer cells by regulating the miR‑153‑3p/PCDHGA9 axis. Thus, HHIP‑AS1 may be a new potential target for lung cancer treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou Z, Lai Y, Cao S, Zhuo Q and Tang H: Long non‑coding RNA HHIP‑AS1 inhibits lung cancer epithelial‑mesenchymal transition and stemness by regulating PCDHGA9. Mol Med Rep 24: 845, 2021.
APA
Zhou, Z., Lai, Y., Cao, S., Zhuo, Q., & Tang, H. (2021). Long non‑coding RNA HHIP‑AS1 inhibits lung cancer epithelial‑mesenchymal transition and stemness by regulating PCDHGA9. Molecular Medicine Reports, 24, 845. https://doi.org/10.3892/mmr.2021.12485
MLA
Zhou, Z., Lai, Y., Cao, S., Zhuo, Q., Tang, H."Long non‑coding RNA HHIP‑AS1 inhibits lung cancer epithelial‑mesenchymal transition and stemness by regulating PCDHGA9". Molecular Medicine Reports 24.6 (2021): 845.
Chicago
Zhou, Z., Lai, Y., Cao, S., Zhuo, Q., Tang, H."Long non‑coding RNA HHIP‑AS1 inhibits lung cancer epithelial‑mesenchymal transition and stemness by regulating PCDHGA9". Molecular Medicine Reports 24, no. 6 (2021): 845. https://doi.org/10.3892/mmr.2021.12485
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Z, Lai Y, Cao S, Zhuo Q and Tang H: Long non‑coding RNA HHIP‑AS1 inhibits lung cancer epithelial‑mesenchymal transition and stemness by regulating PCDHGA9. Mol Med Rep 24: 845, 2021.
APA
Zhou, Z., Lai, Y., Cao, S., Zhuo, Q., & Tang, H. (2021). Long non‑coding RNA HHIP‑AS1 inhibits lung cancer epithelial‑mesenchymal transition and stemness by regulating PCDHGA9. Molecular Medicine Reports, 24, 845. https://doi.org/10.3892/mmr.2021.12485
MLA
Zhou, Z., Lai, Y., Cao, S., Zhuo, Q., Tang, H."Long non‑coding RNA HHIP‑AS1 inhibits lung cancer epithelial‑mesenchymal transition and stemness by regulating PCDHGA9". Molecular Medicine Reports 24.6 (2021): 845.
Chicago
Zhou, Z., Lai, Y., Cao, S., Zhuo, Q., Tang, H."Long non‑coding RNA HHIP‑AS1 inhibits lung cancer epithelial‑mesenchymal transition and stemness by regulating PCDHGA9". Molecular Medicine Reports 24, no. 6 (2021): 845. https://doi.org/10.3892/mmr.2021.12485
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