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Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression

  • Authors:
    • Boshi Yu
    • Mengmeng Li
    • Shu Ping Han
    • Zhangbin Yu
    • Jingai Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Women's Hospital of Nanjing Medical University Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu 210004, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 861
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    Published online on: October 19, 2021
       https://doi.org/10.3892/mmr.2021.12501
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Abstract

Previously it was found that hsa_circ_105039 was underexpressed in the heart tissue of patients with congenital heart disease (CHD). However, the function and mechanism of hsa_circ_105039 in CHD are unclear. In the present study, induced pluripotent stem (iPS) cells were differentiated into cardiomyocytes using 1% dimethyl sulfoxide (DMSO). Cell differentiation, viability, migration and apoptosis were measured before and following hsa_circ_105039 knockdown or overexpression. The results indicated that hsa_circ_105039 overexpression promoted cell differentiation, viability and migration; whereas apoptosis was simultaneously repressed. A luciferase reporter assay verified that hsa_circ_105039 acted as a sponge for microRNA (miR)‑17 and that cyclinD2 was a direct target of miR‑17. Furthermore, differentiation‑related genes and proteins were analyzed by reverse transcription‑quantitative PCR and western blotting, respectively. The results showed that hsa_circ_105039 could also upregulate the expression of differentiation‑related genes and proteins, including natriuretic peptide A, cardiac troponin I, GATA‑binding protein 4 and homobox transcription factor, in iPS cells. The results suggested that hsa_circ_105039 exerted a protective effect by promoting miR‑17/cyclinD2 in DMSO‑induced iPS cardiomyocytes, which indicated that hsa_circ_105039 is a potential key molecule for the diagnosis of CHD.
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Copy and paste a formatted citation
Spandidos Publications style
Yu B, Li M, Han SP, Yu Z and Zhu J: Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression. Mol Med Rep 24: 861, 2021.
APA
Yu, B., Li, M., Han, S.P., Yu, Z., & Zhu, J. (2021). Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression. Molecular Medicine Reports, 24, 861. https://doi.org/10.3892/mmr.2021.12501
MLA
Yu, B., Li, M., Han, S. P., Yu, Z., Zhu, J."Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression". Molecular Medicine Reports 24.6 (2021): 861.
Chicago
Yu, B., Li, M., Han, S. P., Yu, Z., Zhu, J."Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression". Molecular Medicine Reports 24, no. 6 (2021): 861. https://doi.org/10.3892/mmr.2021.12501
Copy and paste a formatted citation
x
Spandidos Publications style
Yu B, Li M, Han SP, Yu Z and Zhu J: Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression. Mol Med Rep 24: 861, 2021.
APA
Yu, B., Li, M., Han, S.P., Yu, Z., & Zhu, J. (2021). Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression. Molecular Medicine Reports, 24, 861. https://doi.org/10.3892/mmr.2021.12501
MLA
Yu, B., Li, M., Han, S. P., Yu, Z., Zhu, J."Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression". Molecular Medicine Reports 24.6 (2021): 861.
Chicago
Yu, B., Li, M., Han, S. P., Yu, Z., Zhu, J."Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression". Molecular Medicine Reports 24, no. 6 (2021): 861. https://doi.org/10.3892/mmr.2021.12501
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